Experiment: Tetracycline 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4813 and Pf6N2E2_4814 are separated by 125 nucleotides Pf6N2E2_4814 and Pf6N2E2_4815 are separated by 348 nucleotides
Pf6N2E2_4813 - Probable two-component sensor, near polyamine transporter, at 5,564,329 to 5,566,725
_4813
Pf6N2E2_4814 - DNA-binding response regulator, LuxR family, near polyamine transporter, at 5,566,851 to 5,567,480
_4814
Pf6N2E2_4815 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 5,567,829 to 5,568,953
_4815
Position (kb) 5566
5567
5568 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5566.006 kb on + strand, within Pf6N2E2_4813 at 5566.006 kb on + strand, within Pf6N2E2_4813 at 5566.007 kb on - strand, within Pf6N2E2_4813 at 5566.007 kb on - strand, within Pf6N2E2_4813 at 5566.007 kb on - strand, within Pf6N2E2_4813 at 5566.007 kb on - strand, within Pf6N2E2_4813 at 5566.007 kb on - strand, within Pf6N2E2_4813 at 5566.007 kb on - strand, within Pf6N2E2_4813 at 5566.007 kb on - strand, within Pf6N2E2_4813 at 5566.191 kb on - strand, within Pf6N2E2_4813 at 5566.191 kb on - strand, within Pf6N2E2_4813 at 5566.191 kb on - strand, within Pf6N2E2_4813 at 5566.191 kb on - strand, within Pf6N2E2_4813 at 5566.191 kb on - strand, within Pf6N2E2_4813 at 5566.251 kb on + strand, within Pf6N2E2_4813 at 5566.251 kb on + strand, within Pf6N2E2_4813 at 5566.251 kb on + strand, within Pf6N2E2_4813 at 5566.251 kb on + strand, within Pf6N2E2_4813 at 5566.252 kb on - strand, within Pf6N2E2_4813 at 5566.252 kb on - strand, within Pf6N2E2_4813 at 5566.254 kb on + strand, within Pf6N2E2_4813 at 5566.254 kb on + strand, within Pf6N2E2_4813 at 5566.254 kb on + strand, within Pf6N2E2_4813 at 5566.255 kb on - strand, within Pf6N2E2_4813 at 5566.255 kb on - strand, within Pf6N2E2_4813 at 5566.255 kb on - strand, within Pf6N2E2_4813 at 5566.255 kb on - strand, within Pf6N2E2_4813 at 5566.255 kb on - strand, within Pf6N2E2_4813 at 5566.255 kb on - strand, within Pf6N2E2_4813 at 5566.383 kb on + strand, within Pf6N2E2_4813 at 5566.509 kb on + strand at 5566.509 kb on + strand at 5566.510 kb on - strand at 5566.510 kb on - strand at 5566.514 kb on + strand at 5566.514 kb on + strand at 5566.515 kb on - strand at 5566.515 kb on - strand at 5566.515 kb on - strand at 5566.574 kb on + strand at 5566.575 kb on - strand at 5566.582 kb on - strand at 5566.590 kb on + strand at 5566.788 kb on + strand at 5566.788 kb on + strand at 5566.788 kb on + strand at 5566.788 kb on + strand at 5566.788 kb on + strand at 5566.788 kb on + strand at 5566.788 kb on + strand at 5566.788 kb on + strand at 5566.788 kb on + strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.861 kb on + strand at 5566.861 kb on + strand at 5566.861 kb on + strand at 5566.861 kb on + strand at 5566.939 kb on + strand, within Pf6N2E2_4814 at 5567.220 kb on + strand, within Pf6N2E2_4814 at 5567.220 kb on + strand, within Pf6N2E2_4814 at 5567.220 kb on + strand, within Pf6N2E2_4814 at 5567.220 kb on + strand, within Pf6N2E2_4814 at 5567.220 kb on + strand, within Pf6N2E2_4814 at 5567.221 kb on - strand, within Pf6N2E2_4814 at 5567.221 kb on - strand, within Pf6N2E2_4814 at 5567.221 kb on - strand, within Pf6N2E2_4814 at 5567.221 kb on - strand, within Pf6N2E2_4814 at 5567.221 kb on - strand, within Pf6N2E2_4814 at 5567.399 kb on + strand, within Pf6N2E2_4814 at 5567.399 kb on + strand, within Pf6N2E2_4814 at 5567.400 kb on - strand, within Pf6N2E2_4814 at 5567.454 kb on + strand at 5567.454 kb on + strand at 5567.455 kb on - strand at 5567.455 kb on - strand at 5567.455 kb on - strand at 5567.455 kb on - strand at 5567.455 kb on - strand at 5567.455 kb on - strand at 5567.478 kb on + strand at 5567.479 kb on - strand at 5567.491 kb on + strand at 5567.492 kb on - strand at 5567.541 kb on + strand at 5567.541 kb on + strand at 5567.558 kb on - strand at 5567.606 kb on + strand at 5567.682 kb on + strand at 5567.682 kb on + strand at 5567.683 kb on - strand at 5567.704 kb on - strand at 5567.786 kb on + strand at 5567.786 kb on + strand at 5567.787 kb on - strand at 5567.813 kb on + strand at 5567.813 kb on + strand at 5567.813 kb on + strand at 5567.829 kb on - strand at 5567.898 kb on + strand at 5567.899 kb on - strand at 5567.899 kb on - strand at 5567.899 kb on - strand at 5568.114 kb on + strand, within Pf6N2E2_4815 at 5568.114 kb on + strand, within Pf6N2E2_4815 at 5568.114 kb on + strand, within Pf6N2E2_4815 at 5568.115 kb on - strand, within Pf6N2E2_4815 at 5568.296 kb on - strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.430 kb on - strand, within Pf6N2E2_4815 at 5568.430 kb on - strand, within Pf6N2E2_4815 at 5568.430 kb on - strand, within Pf6N2E2_4815
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0001 mg/ml remove 5,566,006 + Pf6N2E2_4813 0.70 +0.6 5,566,006 + Pf6N2E2_4813 0.70 -0.1 5,566,007 - Pf6N2E2_4813 0.70 +0.7 5,566,007 - Pf6N2E2_4813 0.70 -0.2 5,566,007 - Pf6N2E2_4813 0.70 +0.2 5,566,007 - Pf6N2E2_4813 0.70 +0.6 5,566,007 - Pf6N2E2_4813 0.70 +0.1 5,566,007 - Pf6N2E2_4813 0.70 -0.0 5,566,007 - Pf6N2E2_4813 0.70 -0.7 5,566,191 - Pf6N2E2_4813 0.78 +0.5 5,566,191 - Pf6N2E2_4813 0.78 -0.1 5,566,191 - Pf6N2E2_4813 0.78 +0.2 5,566,191 - Pf6N2E2_4813 0.78 +0.4 5,566,191 - Pf6N2E2_4813 0.78 +0.4 5,566,251 + Pf6N2E2_4813 0.80 +0.2 5,566,251 + Pf6N2E2_4813 0.80 +0.0 5,566,251 + Pf6N2E2_4813 0.80 +0.5 5,566,251 + Pf6N2E2_4813 0.80 -0.2 5,566,252 - Pf6N2E2_4813 0.80 -0.6 5,566,252 - Pf6N2E2_4813 0.80 -0.2 5,566,254 + Pf6N2E2_4813 0.80 +0.5 5,566,254 + Pf6N2E2_4813 0.80 +0.6 5,566,254 + Pf6N2E2_4813 0.80 -0.3 5,566,255 - Pf6N2E2_4813 0.80 +0.5 5,566,255 - Pf6N2E2_4813 0.80 +0.0 5,566,255 - Pf6N2E2_4813 0.80 -0.3 5,566,255 - Pf6N2E2_4813 0.80 -0.7 5,566,255 - Pf6N2E2_4813 0.80 +0.3 5,566,255 - Pf6N2E2_4813 0.80 +0.3 5,566,383 + Pf6N2E2_4813 0.86 -0.3 5,566,509 + +0.3 5,566,509 + +0.1 5,566,510 - +0.2 5,566,510 - +0.1 5,566,514 + -0.7 5,566,514 + +0.6 5,566,515 - -0.3 5,566,515 - -0.0 5,566,515 - +1.2 5,566,574 + -0.2 5,566,575 - +0.2 5,566,582 - +0.7 5,566,590 + +0.1 5,566,788 + +0.6 5,566,788 + +0.1 5,566,788 + -0.2 5,566,788 + -0.1 5,566,788 + +0.5 5,566,788 + -0.4 5,566,788 + +1.0 5,566,788 + +0.8 5,566,788 + -0.0 5,566,789 - +1.2 5,566,789 - +0.5 5,566,789 - +0.8 5,566,789 - -0.3 5,566,789 - +0.0 5,566,789 - -0.3 5,566,789 - +0.8 5,566,789 - +0.6 5,566,789 - -0.2 5,566,789 - -0.3 5,566,789 - +0.1 5,566,789 - +0.5 5,566,789 - -0.5 5,566,789 - -1.9 5,566,789 - -0.3 5,566,794 + -0.5 5,566,794 + +0.7 5,566,794 + +0.3 5,566,794 + +2.9 5,566,794 + -0.5 5,566,794 + +0.7 5,566,794 + +0.4 5,566,794 + +0.5 5,566,794 + +0.1 5,566,794 + -1.4 5,566,794 + +0.9 5,566,794 + -0.1 5,566,794 + -0.8 5,566,794 + +0.4 5,566,794 + -0.4 5,566,795 - +0.5 5,566,795 - -0.7 5,566,795 - +0.3 5,566,795 - -0.3 5,566,795 - -0.2 5,566,795 - +0.3 5,566,795 - -0.9 5,566,795 - -0.7 5,566,795 - +0.2 5,566,795 - +1.1 5,566,795 - -0.2 5,566,861 + +0.8 5,566,861 + +0.4 5,566,861 + +0.2 5,566,861 + -0.4 5,566,939 + Pf6N2E2_4814 0.14 +1.6 5,567,220 + Pf6N2E2_4814 0.59 +1.2 5,567,220 + Pf6N2E2_4814 0.59 +0.1 5,567,220 + Pf6N2E2_4814 0.59 +0.4 5,567,220 + Pf6N2E2_4814 0.59 +0.3 5,567,220 + Pf6N2E2_4814 0.59 +0.7 5,567,221 - Pf6N2E2_4814 0.59 +0.6 5,567,221 - Pf6N2E2_4814 0.59 -2.0 5,567,221 - Pf6N2E2_4814 0.59 +0.1 5,567,221 - Pf6N2E2_4814 0.59 +0.5 5,567,221 - Pf6N2E2_4814 0.59 -0.0 5,567,399 + Pf6N2E2_4814 0.87 +0.2 5,567,399 + Pf6N2E2_4814 0.87 +0.8 5,567,400 - Pf6N2E2_4814 0.87 +0.0 5,567,454 + +0.6 5,567,454 + -0.6 5,567,455 - +0.2 5,567,455 - +0.5 5,567,455 - +0.3 5,567,455 - -0.6 5,567,455 - +0.2 5,567,455 - +0.4 5,567,478 + -0.0 5,567,479 - +0.1 5,567,491 + +0.6 5,567,492 - -0.0 5,567,541 + -0.1 5,567,541 + +0.3 5,567,558 - +0.8 5,567,606 + +0.8 5,567,682 + -0.5 5,567,682 + -0.5 5,567,683 - -0.0 5,567,704 - +0.1 5,567,786 + -0.0 5,567,786 + -0.1 5,567,787 - -1.0 5,567,813 + +0.4 5,567,813 + -0.4 5,567,813 + +0.1 5,567,829 - -0.3 5,567,898 + -0.2 5,567,899 - -0.1 5,567,899 - -0.3 5,567,899 - -0.0 5,568,114 + Pf6N2E2_4815 0.25 +0.3 5,568,114 + Pf6N2E2_4815 0.25 +0.4 5,568,114 + Pf6N2E2_4815 0.25 -0.2 5,568,115 - Pf6N2E2_4815 0.25 +0.3 5,568,296 - Pf6N2E2_4815 0.42 +0.5 5,568,429 + Pf6N2E2_4815 0.53 -0.3 5,568,429 + Pf6N2E2_4815 0.53 +0.4 5,568,429 + Pf6N2E2_4815 0.53 +0.3 5,568,429 + Pf6N2E2_4815 0.53 +0.6 5,568,429 + Pf6N2E2_4815 0.53 -0.6 5,568,429 + Pf6N2E2_4815 0.53 -0.3 5,568,429 + Pf6N2E2_4815 0.53 +0.2 5,568,429 + Pf6N2E2_4815 0.53 +1.3 5,568,430 - Pf6N2E2_4815 0.53 +0.4 5,568,430 - Pf6N2E2_4815 0.53 +0.1 5,568,430 - Pf6N2E2_4815 0.53 +0.1
Or see this region's nucleotide sequence