Experiment: Tetracycline 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2602 and Pf6N2E2_2603 are separated by 249 nucleotides Pf6N2E2_2603 and Pf6N2E2_2604 are separated by 83 nucleotides
Pf6N2E2_2602 - Flagellin protein FlaA, at 3,059,480 to 3,060,331
_2602
Pf6N2E2_2603 - hypothetical protein, at 3,060,581 to 3,060,763
_2603
Pf6N2E2_2604 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 3,060,847 to 3,062,139
_2604
Position (kb)
3060
3061 Strain fitness (log2 ratio)
-1
0
1
2 at 3059.606 kb on + strand, within Pf6N2E2_2602 at 3059.607 kb on - strand, within Pf6N2E2_2602 at 3059.607 kb on - strand, within Pf6N2E2_2602 at 3059.607 kb on - strand, within Pf6N2E2_2602 at 3059.666 kb on - strand, within Pf6N2E2_2602 at 3059.666 kb on - strand, within Pf6N2E2_2602 at 3059.899 kb on + strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3060.059 kb on - strand, within Pf6N2E2_2602 at 3060.059 kb on - strand, within Pf6N2E2_2602 at 3060.059 kb on - strand, within Pf6N2E2_2602 at 3060.118 kb on + strand, within Pf6N2E2_2602 at 3060.118 kb on + strand, within Pf6N2E2_2602 at 3060.119 kb on - strand, within Pf6N2E2_2602 at 3060.119 kb on - strand, within Pf6N2E2_2602 at 3060.119 kb on - strand, within Pf6N2E2_2602 at 3060.172 kb on + strand, within Pf6N2E2_2602 at 3060.173 kb on - strand, within Pf6N2E2_2602 at 3060.317 kb on + strand at 3060.323 kb on + strand at 3060.393 kb on + strand at 3060.394 kb on - strand at 3060.407 kb on - strand at 3060.409 kb on + strand at 3060.409 kb on + strand at 3060.409 kb on + strand at 3060.410 kb on - strand at 3060.410 kb on - strand at 3060.410 kb on - strand at 3060.413 kb on + strand at 3060.414 kb on - strand at 3060.414 kb on - strand at 3060.437 kb on + strand at 3060.437 kb on + strand at 3060.505 kb on - strand at 3060.622 kb on - strand, within Pf6N2E2_2603 at 3060.622 kb on - strand, within Pf6N2E2_2603 at 3060.622 kb on - strand, within Pf6N2E2_2603 at 3060.631 kb on - strand, within Pf6N2E2_2603 at 3060.671 kb on + strand, within Pf6N2E2_2603 at 3060.671 kb on + strand, within Pf6N2E2_2603 at 3060.672 kb on - strand, within Pf6N2E2_2603 at 3060.672 kb on - strand, within Pf6N2E2_2603 at 3060.672 kb on - strand, within Pf6N2E2_2603 at 3060.672 kb on - strand, within Pf6N2E2_2603 at 3060.672 kb on - strand, within Pf6N2E2_2603 at 3060.686 kb on + strand, within Pf6N2E2_2603 at 3060.686 kb on + strand, within Pf6N2E2_2603 at 3060.687 kb on - strand, within Pf6N2E2_2603 at 3060.687 kb on - strand, within Pf6N2E2_2603 at 3060.687 kb on - strand, within Pf6N2E2_2603 at 3060.687 kb on - strand, within Pf6N2E2_2603 at 3060.687 kb on - strand, within Pf6N2E2_2603 at 3060.858 kb on - strand at 3060.858 kb on - strand at 3060.962 kb on + strand at 3060.962 kb on + strand at 3060.963 kb on - strand at 3061.174 kb on + strand, within Pf6N2E2_2604 at 3061.174 kb on + strand, within Pf6N2E2_2604 at 3061.175 kb on - strand, within Pf6N2E2_2604 at 3061.175 kb on - strand, within Pf6N2E2_2604 at 3061.217 kb on + strand, within Pf6N2E2_2604 at 3061.217 kb on + strand, within Pf6N2E2_2604 at 3061.218 kb on - strand, within Pf6N2E2_2604 at 3061.218 kb on - strand, within Pf6N2E2_2604 at 3061.218 kb on - strand, within Pf6N2E2_2604 at 3061.296 kb on - strand, within Pf6N2E2_2604 at 3061.347 kb on + strand, within Pf6N2E2_2604 at 3061.348 kb on - strand, within Pf6N2E2_2604 at 3061.348 kb on - strand, within Pf6N2E2_2604 at 3061.348 kb on - strand, within Pf6N2E2_2604 at 3061.348 kb on - strand, within Pf6N2E2_2604 at 3061.414 kb on - strand, within Pf6N2E2_2604 at 3061.498 kb on - strand, within Pf6N2E2_2604 at 3061.682 kb on + strand, within Pf6N2E2_2604 at 3061.682 kb on + strand, within Pf6N2E2_2604 at 3061.683 kb on - strand, within Pf6N2E2_2604 at 3061.754 kb on + strand, within Pf6N2E2_2604 at 3061.754 kb on + strand, within Pf6N2E2_2604 at 3061.754 kb on + strand, within Pf6N2E2_2604
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0001 mg/ml remove 3,059,606 + Pf6N2E2_2602 0.15 -1.2 3,059,607 - Pf6N2E2_2602 0.15 +0.2 3,059,607 - Pf6N2E2_2602 0.15 -0.3 3,059,607 - Pf6N2E2_2602 0.15 +0.4 3,059,666 - Pf6N2E2_2602 0.22 +0.0 3,059,666 - Pf6N2E2_2602 0.22 -0.6 3,059,899 + Pf6N2E2_2602 0.49 +0.2 3,059,900 - Pf6N2E2_2602 0.49 +0.8 3,059,900 - Pf6N2E2_2602 0.49 -0.3 3,059,900 - Pf6N2E2_2602 0.49 +0.4 3,059,900 - Pf6N2E2_2602 0.49 +0.5 3,059,900 - Pf6N2E2_2602 0.49 +0.4 3,059,900 - Pf6N2E2_2602 0.49 -0.1 3,060,059 - Pf6N2E2_2602 0.68 +0.5 3,060,059 - Pf6N2E2_2602 0.68 +0.5 3,060,059 - Pf6N2E2_2602 0.68 -0.8 3,060,118 + Pf6N2E2_2602 0.75 -0.4 3,060,118 + Pf6N2E2_2602 0.75 +0.0 3,060,119 - Pf6N2E2_2602 0.75 +0.8 3,060,119 - Pf6N2E2_2602 0.75 +0.7 3,060,119 - Pf6N2E2_2602 0.75 +0.5 3,060,172 + Pf6N2E2_2602 0.81 +1.0 3,060,173 - Pf6N2E2_2602 0.81 +0.7 3,060,317 + +1.6 3,060,323 + +1.9 3,060,393 + +0.3 3,060,394 - +0.4 3,060,407 - +0.7 3,060,409 + +0.2 3,060,409 + +0.3 3,060,409 + +0.2 3,060,410 - -1.0 3,060,410 - +0.3 3,060,410 - +0.8 3,060,413 + +0.5 3,060,414 - +0.0 3,060,414 - +0.0 3,060,437 + +0.0 3,060,437 + +0.9 3,060,505 - +0.6 3,060,622 - Pf6N2E2_2603 0.22 +1.5 3,060,622 - Pf6N2E2_2603 0.22 +0.3 3,060,622 - Pf6N2E2_2603 0.22 -0.2 3,060,631 - Pf6N2E2_2603 0.27 +0.1 3,060,671 + Pf6N2E2_2603 0.49 +1.0 3,060,671 + Pf6N2E2_2603 0.49 -0.1 3,060,672 - Pf6N2E2_2603 0.50 -0.1 3,060,672 - Pf6N2E2_2603 0.50 +0.2 3,060,672 - Pf6N2E2_2603 0.50 +0.3 3,060,672 - Pf6N2E2_2603 0.50 +0.5 3,060,672 - Pf6N2E2_2603 0.50 -0.0 3,060,686 + Pf6N2E2_2603 0.57 +0.8 3,060,686 + Pf6N2E2_2603 0.57 +0.6 3,060,687 - Pf6N2E2_2603 0.58 +0.8 3,060,687 - Pf6N2E2_2603 0.58 +1.0 3,060,687 - Pf6N2E2_2603 0.58 +0.4 3,060,687 - Pf6N2E2_2603 0.58 -0.7 3,060,687 - Pf6N2E2_2603 0.58 +0.2 3,060,858 - +0.1 3,060,858 - +0.7 3,060,962 + -0.1 3,060,962 + -1.0 3,060,963 - -0.0 3,061,174 + Pf6N2E2_2604 0.25 -0.2 3,061,174 + Pf6N2E2_2604 0.25 -0.4 3,061,175 - Pf6N2E2_2604 0.25 -0.5 3,061,175 - Pf6N2E2_2604 0.25 +0.4 3,061,217 + Pf6N2E2_2604 0.29 -0.0 3,061,217 + Pf6N2E2_2604 0.29 +0.1 3,061,218 - Pf6N2E2_2604 0.29 -0.8 3,061,218 - Pf6N2E2_2604 0.29 -0.2 3,061,218 - Pf6N2E2_2604 0.29 +0.0 3,061,296 - Pf6N2E2_2604 0.35 -0.6 3,061,347 + Pf6N2E2_2604 0.39 +1.3 3,061,348 - Pf6N2E2_2604 0.39 -0.0 3,061,348 - Pf6N2E2_2604 0.39 +0.5 3,061,348 - Pf6N2E2_2604 0.39 -0.2 3,061,348 - Pf6N2E2_2604 0.39 -0.3 3,061,414 - Pf6N2E2_2604 0.44 +0.4 3,061,498 - Pf6N2E2_2604 0.50 +0.3 3,061,682 + Pf6N2E2_2604 0.65 -0.9 3,061,682 + Pf6N2E2_2604 0.65 +0.1 3,061,683 - Pf6N2E2_2604 0.65 -0.4 3,061,754 + Pf6N2E2_2604 0.70 +0.6 3,061,754 + Pf6N2E2_2604 0.70 -0.8 3,061,754 + Pf6N2E2_2604 0.70 +0.5
Or see this region's nucleotide sequence