Experiment: Tetracycline 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1505 and Pf6N2E2_1506 are separated by 215 nucleotides Pf6N2E2_1506 and Pf6N2E2_1507 are separated by 89 nucleotides
Pf6N2E2_1505 - Transporter, MFS superfamily, at 1,759,095 to 1,760,291
_1505
Pf6N2E2_1506 - Putative alkanesulfonate metabolism utilization regulator, at 1,760,507 to 1,761,238
_1506
Pf6N2E2_1507 - Sensor protein basS/pmrB (EC 2.7.3.-), at 1,761,328 to 1,762,686
_1507
Position (kb)
1760
1761
1762 Strain fitness (log2 ratio)
-1
0
1 at 1759.588 kb on + strand, within Pf6N2E2_1505 at 1759.901 kb on - strand, within Pf6N2E2_1505 at 1759.957 kb on - strand, within Pf6N2E2_1505 at 1759.957 kb on - strand, within Pf6N2E2_1505 at 1760.158 kb on + strand, within Pf6N2E2_1505 at 1760.295 kb on + strand at 1760.295 kb on + strand at 1760.296 kb on - strand at 1760.296 kb on - strand at 1760.296 kb on - strand at 1760.296 kb on - strand at 1760.650 kb on - strand, within Pf6N2E2_1506 at 1760.721 kb on + strand, within Pf6N2E2_1506 at 1760.722 kb on - strand, within Pf6N2E2_1506 at 1760.849 kb on - strand, within Pf6N2E2_1506 at 1761.105 kb on + strand, within Pf6N2E2_1506 at 1761.106 kb on - strand, within Pf6N2E2_1506 at 1761.201 kb on + strand at 1761.202 kb on - strand at 1761.246 kb on - strand at 1761.269 kb on + strand at 1761.272 kb on - strand at 1761.289 kb on + strand at 1761.289 kb on + strand at 1761.418 kb on + strand at 1761.419 kb on - strand at 1761.626 kb on + strand, within Pf6N2E2_1507 at 1761.627 kb on - strand, within Pf6N2E2_1507 at 1761.627 kb on - strand, within Pf6N2E2_1507 at 1761.693 kb on - strand, within Pf6N2E2_1507 at 1761.926 kb on + strand, within Pf6N2E2_1507 at 1761.966 kb on + strand, within Pf6N2E2_1507 at 1761.966 kb on + strand, within Pf6N2E2_1507 at 1761.966 kb on + strand, within Pf6N2E2_1507 at 1761.967 kb on - strand, within Pf6N2E2_1507 at 1761.967 kb on - strand, within Pf6N2E2_1507 at 1761.967 kb on - strand, within Pf6N2E2_1507 at 1762.139 kb on + strand, within Pf6N2E2_1507 at 1762.230 kb on + strand, within Pf6N2E2_1507 at 1762.231 kb on - strand, within Pf6N2E2_1507 at 1762.231 kb on - strand, within Pf6N2E2_1507
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0001 mg/ml remove 1,759,588 + Pf6N2E2_1505 0.41 +0.5 1,759,901 - Pf6N2E2_1505 0.67 +1.2 1,759,957 - Pf6N2E2_1505 0.72 -0.1 1,759,957 - Pf6N2E2_1505 0.72 +0.2 1,760,158 + Pf6N2E2_1505 0.89 +0.2 1,760,295 + +0.1 1,760,295 + -0.2 1,760,296 - +0.6 1,760,296 - +0.4 1,760,296 - -0.2 1,760,296 - +0.1 1,760,650 - Pf6N2E2_1506 0.20 -0.7 1,760,721 + Pf6N2E2_1506 0.29 +0.6 1,760,722 - Pf6N2E2_1506 0.29 +0.1 1,760,849 - Pf6N2E2_1506 0.47 -0.6 1,761,105 + Pf6N2E2_1506 0.82 -1.1 1,761,106 - Pf6N2E2_1506 0.82 -1.3 1,761,201 + +0.7 1,761,202 - +0.2 1,761,246 - -0.3 1,761,269 + +0.5 1,761,272 - +0.4 1,761,289 + +0.9 1,761,289 + +0.0 1,761,418 + +0.1 1,761,419 - +0.7 1,761,626 + Pf6N2E2_1507 0.22 +1.2 1,761,627 - Pf6N2E2_1507 0.22 +0.3 1,761,627 - Pf6N2E2_1507 0.22 -0.5 1,761,693 - Pf6N2E2_1507 0.27 -0.6 1,761,926 + Pf6N2E2_1507 0.44 +0.4 1,761,966 + Pf6N2E2_1507 0.47 +0.2 1,761,966 + Pf6N2E2_1507 0.47 +0.7 1,761,966 + Pf6N2E2_1507 0.47 +0.8 1,761,967 - Pf6N2E2_1507 0.47 -0.5 1,761,967 - Pf6N2E2_1507 0.47 +0.5 1,761,967 - Pf6N2E2_1507 0.47 +0.3 1,762,139 + Pf6N2E2_1507 0.60 +0.4 1,762,230 + Pf6N2E2_1507 0.66 +0.3 1,762,231 - Pf6N2E2_1507 0.66 -0.2 1,762,231 - Pf6N2E2_1507 0.66 -0.7
Or see this region's nucleotide sequence