Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31960

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31955 and BPHYT_RS31960 are separated by 206 nucleotidesBPHYT_RS31960 and BPHYT_RS31965 are separated by 98 nucleotides BPHYT_RS31955: BPHYT_RS31955 - 3-hydroxyisobutyrate dehydrogenase, at 2,729,015 to 2,729,917 _RS31955 BPHYT_RS31960: BPHYT_RS31960 - hypothetical protein, at 2,730,124 to 2,731,071 _RS31960 BPHYT_RS31965: BPHYT_RS31965 - mandelate racemase, at 2,731,170 to 2,732,303 _RS31965 Position (kb) 2730 2731 2732Strain fitness (log2 ratio) -2 -1 0 1at 2729.165 kb on - strand, within BPHYT_RS31955at 2729.165 kb on - strand, within BPHYT_RS31955at 2729.174 kb on - strand, within BPHYT_RS31955at 2729.243 kb on - strand, within BPHYT_RS31955at 2729.255 kb on - strand, within BPHYT_RS31955at 2729.298 kb on - strand, within BPHYT_RS31955at 2729.414 kb on + strand, within BPHYT_RS31955at 2729.415 kb on - strand, within BPHYT_RS31955at 2729.441 kb on + strand, within BPHYT_RS31955at 2729.442 kb on - strand, within BPHYT_RS31955at 2729.442 kb on - strand, within BPHYT_RS31955at 2729.442 kb on - strand, within BPHYT_RS31955at 2729.455 kb on - strand, within BPHYT_RS31955at 2729.455 kb on - strand, within BPHYT_RS31955at 2729.510 kb on - strand, within BPHYT_RS31955at 2729.735 kb on + strand, within BPHYT_RS31955at 2729.827 kb on - strandat 2730.222 kb on + strand, within BPHYT_RS31960at 2730.667 kb on + strand, within BPHYT_RS31960at 2730.758 kb on + strand, within BPHYT_RS31960at 2730.759 kb on - strand, within BPHYT_RS31960at 2731.081 kb on + strandat 2731.120 kb on + strandat 2731.145 kb on + strandat 2731.145 kb on + strandat 2731.277 kb on - strandat 2731.494 kb on - strand, within BPHYT_RS31965at 2731.657 kb on - strand, within BPHYT_RS31965at 2731.760 kb on + strand, within BPHYT_RS31965at 2731.764 kb on - strand, within BPHYT_RS31965at 2731.764 kb on - strand, within BPHYT_RS31965at 2731.771 kb on - strand, within BPHYT_RS31965at 2731.777 kb on + strand, within BPHYT_RS31965at 2731.796 kb on - strand, within BPHYT_RS31965at 2731.945 kb on + strand, within BPHYT_RS31965at 2732.038 kb on + strand, within BPHYT_RS31965

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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2,729,165 - BPHYT_RS31955 0.17 -0.2
2,729,165 - BPHYT_RS31955 0.17 -0.3
2,729,174 - BPHYT_RS31955 0.18 -0.8
2,729,243 - BPHYT_RS31955 0.25 +0.6
2,729,255 - BPHYT_RS31955 0.27 +0.8
2,729,298 - BPHYT_RS31955 0.31 +0.3
2,729,414 + BPHYT_RS31955 0.44 -1.5
2,729,415 - BPHYT_RS31955 0.44 +0.1
2,729,441 + BPHYT_RS31955 0.47 -1.9
2,729,442 - BPHYT_RS31955 0.47 +0.9
2,729,442 - BPHYT_RS31955 0.47 -1.1
2,729,442 - BPHYT_RS31955 0.47 +1.3
2,729,455 - BPHYT_RS31955 0.49 +0.1
2,729,455 - BPHYT_RS31955 0.49 +0.5
2,729,510 - BPHYT_RS31955 0.55 +0.2
2,729,735 + BPHYT_RS31955 0.80 +1.5
2,729,827 - -0.4
2,730,222 + BPHYT_RS31960 0.10 +0.1
2,730,667 + BPHYT_RS31960 0.57 -1.1
2,730,758 + BPHYT_RS31960 0.67 -1.3
2,730,759 - BPHYT_RS31960 0.67 -2.0
2,731,081 + +1.7
2,731,120 + +0.2
2,731,145 + +0.1
2,731,145 + -0.1
2,731,277 - -0.0
2,731,494 - BPHYT_RS31965 0.29 -1.3
2,731,657 - BPHYT_RS31965 0.43 +0.3
2,731,760 + BPHYT_RS31965 0.52 +0.1
2,731,764 - BPHYT_RS31965 0.52 -0.5
2,731,764 - BPHYT_RS31965 0.52 +0.2
2,731,771 - BPHYT_RS31965 0.53 -0.3
2,731,777 + BPHYT_RS31965 0.54 +0.2
2,731,796 - BPHYT_RS31965 0.55 +0.0
2,731,945 + BPHYT_RS31965 0.68 -0.1
2,732,038 + BPHYT_RS31965 0.77 +0.1

Or see this region's nucleotide sequence