Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28625 and BPHYT_RS28630 are separated by 13 nucleotides BPHYT_RS28630 and BPHYT_RS28635 are separated by 87 nucleotides BPHYT_RS28635 and BPHYT_RS28640 are separated by 463 nucleotides
BPHYT_RS28625: BPHYT_RS28625 - major facilitator transporter, at 1,976,474 to 1,977,805
_RS28625
BPHYT_RS28630: BPHYT_RS28630 - IclR family transcriptional regulator, at 1,977,819 to 1,978,610
_RS28630
BPHYT_RS28635: BPHYT_RS28635 - hypothetical protein, at 1,978,698 to 1,978,919
_RS28635
BPHYT_RS28640: BPHYT_RS28640 - ATPase, at 1,979,383 to 1,981,047
_RS28640
Position (kb) 1978
1979 Strain fitness (log2 ratio) -2
-1
0
1 at 1977.725 kb on + strand at 1978.388 kb on - strand, within BPHYT_RS28630 at 1978.417 kb on + strand, within BPHYT_RS28630 at 1978.589 kb on + strand at 1978.699 kb on + strand at 1978.852 kb on - strand, within BPHYT_RS28635 at 1978.852 kb on - strand, within BPHYT_RS28635 at 1979.035 kb on + strand at 1979.335 kb on - strand at 1979.776 kb on - strand, within BPHYT_RS28640 at 1979.803 kb on + strand, within BPHYT_RS28640
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,977,725 + -1.2 1,978,388 - BPHYT_RS28630 0.72 +0.7 1,978,417 + BPHYT_RS28630 0.76 -0.6 1,978,589 + -0.0 1,978,699 + -0.1 1,978,852 - BPHYT_RS28635 0.69 +1.7 1,978,852 - BPHYT_RS28635 0.69 +0.1 1,979,035 + +1.0 1,979,335 - +0.1 1,979,776 - BPHYT_RS28640 0.24 +0.6 1,979,803 + BPHYT_RS28640 0.25 -2.6
Or see this region's nucleotide sequence