Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28135 and BPHYT_RS28140 are separated by 337 nucleotides BPHYT_RS28140 and BPHYT_RS28145 are separated by 57 nucleotides BPHYT_RS28145 and BPHYT_RS28150 are separated by 199 nucleotides
BPHYT_RS28135: BPHYT_RS28135 - alcohol dehydrogenase, at 1,870,175 to 1,871,194
_RS28135
BPHYT_RS28140: BPHYT_RS28140 - translation initiation factor SUI1, at 1,871,532 to 1,871,894
_RS28140
BPHYT_RS28145: BPHYT_RS28145 - diguanylate cyclase, at 1,871,952 to 1,873,133
_RS28145
BPHYT_RS28150: BPHYT_RS28150 - methylmalonate-semialdehyde dehydrogenase, at 1,873,333 to 1,874,850
_RS28150
Position (kb)
1871
1872
1873
1874 Strain fitness (log2 ratio)
-2
-1
0
1 at 1870.985 kb on - strand, within BPHYT_RS28135 at 1871.362 kb on + strand at 1871.387 kb on + strand at 1871.388 kb on - strand at 1871.388 kb on - strand at 1871.445 kb on - strand at 1871.670 kb on + strand, within BPHYT_RS28140 at 1871.917 kb on + strand at 1871.917 kb on + strand at 1871.918 kb on - strand at 1871.918 kb on - strand at 1872.117 kb on + strand, within BPHYT_RS28145 at 1872.118 kb on - strand, within BPHYT_RS28145 at 1872.391 kb on - strand, within BPHYT_RS28145 at 1872.559 kb on + strand, within BPHYT_RS28145 at 1872.560 kb on - strand, within BPHYT_RS28145 at 1872.560 kb on - strand at 1872.643 kb on + strand, within BPHYT_RS28145 at 1872.781 kb on - strand, within BPHYT_RS28145 at 1873.023 kb on + strand at 1873.024 kb on - strand at 1873.101 kb on - strand at 1873.108 kb on + strand at 1873.109 kb on - strand at 1873.152 kb on - strand at 1873.305 kb on - strand at 1873.406 kb on + strand at 1873.407 kb on - strand at 1873.414 kb on - strand at 1873.581 kb on + strand, within BPHYT_RS28150 at 1874.007 kb on - strand, within BPHYT_RS28150 at 1874.133 kb on - strand, within BPHYT_RS28150
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,870,985 - BPHYT_RS28135 0.79 -0.7 1,871,362 + +0.7 1,871,387 + +0.8 1,871,388 - -2.4 1,871,388 - -0.7 1,871,445 - +0.1 1,871,670 + BPHYT_RS28140 0.38 +0.3 1,871,917 + -0.6 1,871,917 + -0.6 1,871,918 - -0.8 1,871,918 - -1.0 1,872,117 + BPHYT_RS28145 0.14 -1.2 1,872,118 - BPHYT_RS28145 0.14 +0.4 1,872,391 - BPHYT_RS28145 0.37 -0.2 1,872,559 + BPHYT_RS28145 0.51 -0.7 1,872,560 - BPHYT_RS28145 0.51 -2.2 1,872,560 - +1.3 1,872,643 + BPHYT_RS28145 0.58 -1.4 1,872,781 - BPHYT_RS28145 0.70 -1.3 1,873,023 + +0.4 1,873,024 - +0.5 1,873,101 - -0.1 1,873,108 + +0.8 1,873,109 - +0.3 1,873,152 - -2.1 1,873,305 - +0.1 1,873,406 + +1.5 1,873,407 - +0.2 1,873,414 - -1.6 1,873,581 + BPHYT_RS28150 0.16 -0.7 1,874,007 - BPHYT_RS28150 0.44 +0.0 1,874,133 - BPHYT_RS28150 0.53 +0.7
Or see this region's nucleotide sequence