Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25715 and BPHYT_RS25720 are separated by 354 nucleotides BPHYT_RS25720 and BPHYT_RS25725 are separated by 98 nucleotides BPHYT_RS25725 and BPHYT_RS25730 overlap by 8 nucleotides
BPHYT_RS25715: BPHYT_RS25715 - transposase, at 1,341,829 to 1,343,262
_RS25715
BPHYT_RS25720: BPHYT_RS25720 - hypothetical protein, at 1,343,617 to 1,344,117
_RS25720
BPHYT_RS25725: BPHYT_RS25725 - hypothetical protein, at 1,344,216 to 1,344,713
_RS25725
BPHYT_RS25730: BPHYT_RS25730 - hypothetical protein, at 1,344,706 to 1,347,030
_RS25730
Position (kb) 1343
1344
1345 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1343.111 kb on + strand, within BPHYT_RS25715 at 1343.327 kb on - strand at 1343.331 kb on - strand at 1343.347 kb on + strand at 1343.390 kb on - strand at 1343.444 kb on + strand at 1343.676 kb on - strand, within BPHYT_RS25720 at 1343.680 kb on - strand, within BPHYT_RS25720 at 1343.879 kb on + strand, within BPHYT_RS25720 at 1343.879 kb on + strand, within BPHYT_RS25720 at 1343.879 kb on + strand, within BPHYT_RS25720 at 1343.920 kb on - strand, within BPHYT_RS25720 at 1344.123 kb on - strand at 1344.123 kb on - strand at 1344.408 kb on + strand, within BPHYT_RS25725 at 1344.535 kb on + strand at 1344.800 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,343,111 + BPHYT_RS25715 0.89 -0.9 1,343,327 - -2.7 1,343,331 - -1.3 1,343,347 + -0.2 1,343,390 - -0.1 1,343,444 + +0.3 1,343,676 - BPHYT_RS25720 0.12 -2.8 1,343,680 - BPHYT_RS25720 0.13 -0.0 1,343,879 + BPHYT_RS25720 0.52 -2.4 1,343,879 + BPHYT_RS25720 0.52 +0.0 1,343,879 + BPHYT_RS25720 0.52 -0.9 1,343,920 - BPHYT_RS25720 0.60 -0.1 1,344,123 - +0.9 1,344,123 - +0.3 1,344,408 + BPHYT_RS25725 0.39 -2.6 1,344,535 + +1.3 1,344,800 - +1.0
Or see this region's nucleotide sequence