Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20885 and BPHYT_RS20890 are separated by 94 nucleotides BPHYT_RS20890 and BPHYT_RS20895 are separated by 345 nucleotides BPHYT_RS20895 and BPHYT_RS20900 are separated by 319 nucleotides
BPHYT_RS20885: BPHYT_RS20885 - nuclear protein SET, at 246,679 to 247,119
_RS20885
BPHYT_RS20890: BPHYT_RS20890 - transglycosylase, at 247,214 to 247,465
_RS20890
BPHYT_RS20895: BPHYT_RS20895 - ABC transporter, at 247,811 to 249,604
_RS20895
BPHYT_RS20900: BPHYT_RS20900 - phytanoyl-CoA dioxygenase, at 249,924 to 251,138
_RS20900
Position (kb) 247
248
249
250 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 247.005 kb on + strand, within BPHYT_RS20885 at 247.013 kb on - strand, within BPHYT_RS20885 at 247.060 kb on + strand, within BPHYT_RS20885 at 247.122 kb on + strand at 247.271 kb on - strand, within BPHYT_RS20890 at 247.372 kb on + strand, within BPHYT_RS20890 at 247.575 kb on + strand at 247.575 kb on + strand at 247.575 kb on + strand at 247.576 kb on - strand at 247.664 kb on - strand at 247.787 kb on + strand at 247.970 kb on + strand at 248.035 kb on - strand, within BPHYT_RS20895 at 248.193 kb on + strand, within BPHYT_RS20895 at 248.194 kb on - strand, within BPHYT_RS20895 at 248.365 kb on + strand, within BPHYT_RS20895 at 248.366 kb on - strand, within BPHYT_RS20895 at 248.482 kb on + strand, within BPHYT_RS20895 at 248.733 kb on + strand, within BPHYT_RS20895 at 248.733 kb on + strand, within BPHYT_RS20895 at 248.880 kb on - strand, within BPHYT_RS20895 at 249.690 kb on + strand at 249.819 kb on + strand at 249.897 kb on + strand at 249.945 kb on - strand at 250.486 kb on - strand, within BPHYT_RS20900
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 247,005 + BPHYT_RS20885 0.74 -0.8 247,013 - BPHYT_RS20885 0.76 -0.5 247,060 + BPHYT_RS20885 0.86 -2.8 247,122 + +0.4 247,271 - BPHYT_RS20890 0.23 -0.4 247,372 + BPHYT_RS20890 0.63 -0.5 247,575 + +1.5 247,575 + -0.2 247,575 + -1.3 247,576 - +0.1 247,664 - +0.2 247,787 + -1.4 247,970 + -1.1 248,035 - BPHYT_RS20895 0.12 +0.8 248,193 + BPHYT_RS20895 0.21 -1.3 248,194 - BPHYT_RS20895 0.21 -0.0 248,365 + BPHYT_RS20895 0.31 -0.2 248,366 - BPHYT_RS20895 0.31 -4.0 248,482 + BPHYT_RS20895 0.37 +0.1 248,733 + BPHYT_RS20895 0.51 -0.6 248,733 + BPHYT_RS20895 0.51 +0.1 248,880 - BPHYT_RS20895 0.60 +0.5 249,690 + -0.7 249,819 + +0.6 249,897 + +2.3 249,945 - -0.4 250,486 - BPHYT_RS20900 0.46 +1.2
Or see this region's nucleotide sequence