Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS06480 and BPHYT_RS06485 are separated by 26 nucleotides BPHYT_RS06485 and BPHYT_RS06490 overlap by 8 nucleotides BPHYT_RS06490 and BPHYT_RS06495 are separated by 164 nucleotides BPHYT_RS06495 and BPHYT_RS06500 are separated by 32 nucleotides
BPHYT_RS06480: BPHYT_RS06480 - ABC transporter permease, at 1,473,691 to 1,476,180
_RS06480
BPHYT_RS06485: BPHYT_RS06485 - globin, at 1,476,207 to 1,476,620
_RS06485
BPHYT_RS06490: BPHYT_RS06490 - membrane protein, at 1,476,613 to 1,477,242
_RS06490
BPHYT_RS06495: BPHYT_RS06495 - glucose-1-dehydrogenase, at 1,477,407 to 1,478,153
_RS06495
BPHYT_RS06500: BPHYT_RS06500 - LOG family protein, at 1,478,186 to 1,478,770
_RS06500
Position (kb) 1476
1477
1478 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1475.854 kb on + strand, within BPHYT_RS06480 at 1475.887 kb on - strand, within BPHYT_RS06480 at 1475.887 kb on - strand, within BPHYT_RS06480 at 1476.019 kb on + strand at 1476.019 kb on + strand at 1476.049 kb on + strand at 1476.050 kb on - strand at 1476.207 kb on - strand at 1476.426 kb on + strand, within BPHYT_RS06485 at 1476.653 kb on - strand at 1476.906 kb on + strand, within BPHYT_RS06490 at 1476.907 kb on - strand, within BPHYT_RS06490 at 1477.015 kb on + strand, within BPHYT_RS06490 at 1477.015 kb on + strand, within BPHYT_RS06490 at 1477.082 kb on + strand, within BPHYT_RS06490 at 1477.108 kb on + strand, within BPHYT_RS06490 at 1477.261 kb on + strand at 1477.261 kb on + strand at 1477.408 kb on + strand at 1477.408 kb on + strand at 1477.447 kb on + strand at 1477.473 kb on + strand at 1477.473 kb on + strand at 1477.474 kb on - strand at 1477.474 kb on - strand at 1477.474 kb on - strand at 1477.658 kb on + strand, within BPHYT_RS06495 at 1477.693 kb on + strand, within BPHYT_RS06495 at 1477.693 kb on + strand, within BPHYT_RS06495 at 1477.804 kb on + strand, within BPHYT_RS06495 at 1477.805 kb on - strand, within BPHYT_RS06495
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,475,854 + BPHYT_RS06480 0.87 +0.0 1,475,887 - BPHYT_RS06480 0.88 -2.1 1,475,887 - BPHYT_RS06480 0.88 +1.0 1,476,019 + +1.2 1,476,019 + -0.1 1,476,049 + -0.2 1,476,050 - +1.4 1,476,207 - +1.3 1,476,426 + BPHYT_RS06485 0.53 -0.9 1,476,653 - -0.0 1,476,906 + BPHYT_RS06490 0.47 +1.4 1,476,907 - BPHYT_RS06490 0.47 +0.1 1,477,015 + BPHYT_RS06490 0.64 +1.3 1,477,015 + BPHYT_RS06490 0.64 -1.1 1,477,082 + BPHYT_RS06490 0.74 -0.7 1,477,108 + BPHYT_RS06490 0.79 -0.2 1,477,261 + -0.1 1,477,261 + +2.0 1,477,408 + +0.4 1,477,408 + +0.6 1,477,447 + -1.1 1,477,473 + +0.5 1,477,473 + -0.1 1,477,474 - +0.5 1,477,474 - -0.2 1,477,474 - -0.2 1,477,658 + BPHYT_RS06495 0.34 -0.9 1,477,693 + BPHYT_RS06495 0.38 -0.2 1,477,693 + BPHYT_RS06495 0.38 -3.6 1,477,804 + BPHYT_RS06495 0.53 +0.6 1,477,805 - BPHYT_RS06495 0.53 -1.4
Or see this region's nucleotide sequence