Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04875 and BPHYT_RS04880 are separated by 29 nucleotides BPHYT_RS04880 and BPHYT_RS04885 are separated by 62 nucleotides BPHYT_RS04885 and BPHYT_RS04890 are separated by 17 nucleotides BPHYT_RS04890 and BPHYT_RS04895 overlap by 1 nucleotides
BPHYT_RS04875: BPHYT_RS04875 - alpha-ribazole phosphatase, at 1,122,320 to 1,122,910
_RS04875
BPHYT_RS04880: BPHYT_RS04880 - cobalamin-binding protein, at 1,122,940 to 1,123,842
_RS04880
BPHYT_RS04885: BPHYT_RS04885 - threonine-phosphate decarboxylase, at 1,123,905 to 1,124,954
_RS04885
BPHYT_RS04890: BPHYT_RS04890 - cobalamin biosynthesis protein CobD, at 1,124,972 to 1,125,907
_RS04890
BPHYT_RS04895: BPHYT_RS04895 - adenosylcobinamide kinase, at 1,125,907 to 1,126,461
_RS04895
Position (kb) 1123
1124
1125 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1122.922 kb on + strand at 1122.922 kb on + strand at 1122.922 kb on + strand at 1122.923 kb on - strand at 1122.923 kb on - strand at 1122.928 kb on - strand at 1122.928 kb on - strand at 1123.316 kb on + strand, within BPHYT_RS04880 at 1123.379 kb on + strand, within BPHYT_RS04880 at 1123.688 kb on + strand, within BPHYT_RS04880 at 1123.688 kb on + strand, within BPHYT_RS04880 at 1123.688 kb on + strand, within BPHYT_RS04880 at 1123.689 kb on - strand, within BPHYT_RS04880 at 1123.865 kb on - strand at 1123.865 kb on - strand at 1124.620 kb on + strand, within BPHYT_RS04885 at 1124.624 kb on + strand, within BPHYT_RS04885 at 1124.831 kb on + strand, within BPHYT_RS04885 at 1125.038 kb on - strand at 1125.268 kb on - strand, within BPHYT_RS04890 at 1125.268 kb on - strand, within BPHYT_RS04890
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,122,922 + +1.1 1,122,922 + +0.6 1,122,922 + +0.4 1,122,923 - -1.2 1,122,923 - +0.6 1,122,928 - -2.2 1,122,928 - -0.3 1,123,316 + BPHYT_RS04880 0.42 -0.2 1,123,379 + BPHYT_RS04880 0.49 +0.3 1,123,688 + BPHYT_RS04880 0.83 -0.9 1,123,688 + BPHYT_RS04880 0.83 -0.4 1,123,688 + BPHYT_RS04880 0.83 -0.5 1,123,689 - BPHYT_RS04880 0.83 +0.0 1,123,865 - +0.4 1,123,865 - -3.2 1,124,620 + BPHYT_RS04885 0.68 -1.5 1,124,624 + BPHYT_RS04885 0.68 +0.7 1,124,831 + BPHYT_RS04885 0.88 +0.6 1,125,038 - +0.4 1,125,268 - BPHYT_RS04890 0.32 -2.1 1,125,268 - BPHYT_RS04890 0.32 -3.0
Or see this region's nucleotide sequence