Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02780 and BPHYT_RS02785 are separated by 42 nucleotides BPHYT_RS02785 and BPHYT_RS02790 are separated by 192 nucleotides BPHYT_RS02790 and BPHYT_RS02795 are separated by 508 nucleotides
BPHYT_RS02780: BPHYT_RS02780 - deacylase, at 624,538 to 625,101
_RS02780
BPHYT_RS02785: BPHYT_RS02785 - hypothetical protein, at 625,144 to 625,527
_RS02785
BPHYT_RS02790: BPHYT_RS02790 - hypothetical protein, at 625,720 to 626,763
_RS02790
BPHYT_RS02795: BPHYT_RS02795 - glycosyl transferase, at 627,272 to 628,243
_RS02795
Position (kb) 625
626
627 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 625.001 kb on - strand, within BPHYT_RS02780 at 625.065 kb on - strand at 625.079 kb on + strand at 625.079 kb on + strand at 625.079 kb on + strand at 625.079 kb on + strand at 625.079 kb on + strand at 625.080 kb on - strand at 625.081 kb on + strand at 625.081 kb on + strand at 625.091 kb on - strand at 625.255 kb on + strand, within BPHYT_RS02785 at 625.256 kb on - strand, within BPHYT_RS02785 at 625.256 kb on - strand, within BPHYT_RS02785 at 625.263 kb on + strand, within BPHYT_RS02785 at 625.264 kb on - strand, within BPHYT_RS02785 at 625.450 kb on + strand, within BPHYT_RS02785 at 625.464 kb on + strand, within BPHYT_RS02785 at 625.464 kb on + strand, within BPHYT_RS02785 at 625.790 kb on + strand at 625.831 kb on + strand, within BPHYT_RS02790 at 625.831 kb on + strand, within BPHYT_RS02790 at 625.832 kb on - strand, within BPHYT_RS02790 at 626.244 kb on - strand, within BPHYT_RS02790 at 626.381 kb on + strand, within BPHYT_RS02790 at 626.381 kb on + strand, within BPHYT_RS02790 at 626.382 kb on - strand, within BPHYT_RS02790 at 626.988 kb on - strand at 627.005 kb on - strand at 627.005 kb on - strand at 627.106 kb on - strand at 627.110 kb on + strand at 627.111 kb on - strand at 627.117 kb on - strand at 627.117 kb on - strand at 627.184 kb on + strand at 627.184 kb on + strand at 627.184 kb on + strand at 627.184 kb on + strand at 627.185 kb on - strand at 627.185 kb on - strand at 627.185 kb on - strand at 627.189 kb on + strand at 627.190 kb on - strand at 627.190 kb on - strand at 627.190 kb on - strand at 627.499 kb on + strand, within BPHYT_RS02795 at 627.611 kb on - strand, within BPHYT_RS02795 at 627.611 kb on - strand, within BPHYT_RS02795 at 627.612 kb on + strand, within BPHYT_RS02795 at 627.612 kb on + strand, within BPHYT_RS02795 at 627.612 kb on + strand, within BPHYT_RS02795 at 627.613 kb on - strand, within BPHYT_RS02795 at 627.613 kb on - strand, within BPHYT_RS02795 at 627.613 kb on - strand, within BPHYT_RS02795
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 625,001 - BPHYT_RS02780 0.82 +0.9 625,065 - +0.4 625,079 + +0.4 625,079 + +0.3 625,079 + -0.1 625,079 + +1.0 625,079 + +1.8 625,080 - +0.4 625,081 + +0.6 625,081 + -1.9 625,091 - +0.3 625,255 + BPHYT_RS02785 0.29 -0.5 625,256 - BPHYT_RS02785 0.29 +1.0 625,256 - BPHYT_RS02785 0.29 +0.6 625,263 + BPHYT_RS02785 0.31 -0.3 625,264 - BPHYT_RS02785 0.31 -0.9 625,450 + BPHYT_RS02785 0.80 +0.4 625,464 + BPHYT_RS02785 0.83 +0.5 625,464 + BPHYT_RS02785 0.83 -1.4 625,790 + -0.9 625,831 + BPHYT_RS02790 0.11 -2.8 625,831 + BPHYT_RS02790 0.11 -1.0 625,832 - BPHYT_RS02790 0.11 +0.2 626,244 - BPHYT_RS02790 0.50 -2.5 626,381 + BPHYT_RS02790 0.63 -1.7 626,381 + BPHYT_RS02790 0.63 -0.1 626,382 - BPHYT_RS02790 0.63 -0.8 626,988 - +0.2 627,005 - -0.2 627,005 - +0.1 627,106 - +2.2 627,110 + +0.0 627,111 - +0.1 627,117 - -0.6 627,117 - +0.5 627,184 + -0.1 627,184 + -2.1 627,184 + -1.5 627,184 + -0.3 627,185 - -0.5 627,185 - -0.2 627,185 - +0.0 627,189 + -0.3 627,190 - -0.1 627,190 - +0.0 627,190 - -0.7 627,499 + BPHYT_RS02795 0.23 +1.1 627,611 - BPHYT_RS02795 0.35 -2.4 627,611 - BPHYT_RS02795 0.35 +0.4 627,612 + BPHYT_RS02795 0.35 +0.1 627,612 + BPHYT_RS02795 0.35 -1.3 627,612 + BPHYT_RS02795 0.35 -1.2 627,613 - BPHYT_RS02795 0.35 +0.1 627,613 - BPHYT_RS02795 0.35 -2.1 627,613 - BPHYT_RS02795 0.35 -0.4
Or see this region's nucleotide sequence