Experiment: Fusidic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS21770 and ABZR86_RS21775 are separated by 23 nucleotides ABZR86_RS21775 and ABZR86_RS21780 are separated by 199 nucleotides ABZR86_RS21780 and ABZR86_RS21785 are separated by 210 nucleotides
ABZR86_RS21770: ABZR86_RS21770 - class II 3-deoxy-7-phosphoheptulonate synthase, at 328,735 to 330,108
_RS21770
ABZR86_RS21775: ABZR86_RS21775 - disulfide bond formation protein B, at 330,132 to 330,635
_RS21775
ABZR86_RS21780: ABZR86_RS21780 - 50S ribosomal protein L17, at 330,835 to 331,218
_RS21780
ABZR86_RS21785: ABZR86_RS21785 - DNA-directed RNA polymerase subunit alpha, at 331,429 to 332,427
_RS21785
Position (kb) 330
331
332 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 330.710 kb on - strand at 330.812 kb on + strand at 330.812 kb on + strand at 330.836 kb on + strand at 330.836 kb on + strand at 330.836 kb on + strand at 330.837 kb on - strand at 331.233 kb on - strand at 331.245 kb on - strand at 331.301 kb on - strand at 331.301 kb on - strand at 331.301 kb on - strand at 331.301 kb on - strand at 331.301 kb on - strand at 331.315 kb on - strand at 331.315 kb on - strand at 331.317 kb on - strand at 331.317 kb on - strand at 331.317 kb on - strand at 331.355 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.4 mg/ml remove 330,710 - +0.2 330,812 + -0.3 330,812 + +0.3 330,836 + +0.2 330,836 + -0.4 330,836 + +0.7 330,837 - +4.2 331,233 - +0.3 331,245 - +0.5 331,301 - +0.5 331,301 - +0.6 331,301 - +0.5 331,301 - -0.6 331,301 - +0.2 331,315 - +1.0 331,315 - +0.9 331,317 - +1.3 331,317 - +1.4 331,317 - +1.2 331,355 - +0.5
Or see this region's nucleotide sequence