Experiment: Fusidic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS10440 and ABZR86_RS10445 are separated by 36 nucleotides ABZR86_RS10445 and ABZR86_RS10450 are separated by 39 nucleotides ABZR86_RS10450 and ABZR86_RS10455 are separated by 36 nucleotides ABZR86_RS10455 and ABZR86_RS10460 are separated by 370 nucleotides
ABZR86_RS10440: ABZR86_RS10440 - secondary thiamine-phosphate synthase enzyme YjbQ, at 2,324,630 to 2,325,070
_RS10440
ABZR86_RS10445: ABZR86_RS10445 - pyridoxamine 5'-phosphate oxidase, at 2,325,107 to 2,325,694
_RS10445
ABZR86_RS10450: ABZR86_RS10450 - kinase, at 2,325,734 to 2,326,564
_RS10450
ABZR86_RS10455: ABZR86_RS10455 - heavy metal-binding domain-containing protein, at 2,326,601 to 2,326,921
_RS10455
ABZR86_RS10460: ABZR86_RS10460 - hypothetical protein, at 2,327,292 to 2,328,014
_RS10460
Position (kb)
2325
2326
2327 Strain fitness (log2 ratio)
-2
-1
0
1 at 2324.917 kb on + strand, within ABZR86_RS10440 at 2324.917 kb on + strand, within ABZR86_RS10440 at 2325.016 kb on + strand, within ABZR86_RS10440 at 2325.017 kb on - strand, within ABZR86_RS10440 at 2325.748 kb on + strand at 2325.748 kb on + strand at 2325.748 kb on + strand at 2325.748 kb on + strand at 2325.748 kb on + strand at 2325.748 kb on + strand at 2325.791 kb on + strand at 2325.792 kb on - strand at 2325.825 kb on - strand, within ABZR86_RS10450 at 2325.825 kb on - strand, within ABZR86_RS10450 at 2325.941 kb on + strand, within ABZR86_RS10450 at 2325.941 kb on + strand, within ABZR86_RS10450 at 2325.942 kb on - strand, within ABZR86_RS10450 at 2325.945 kb on - strand, within ABZR86_RS10450 at 2325.945 kb on - strand, within ABZR86_RS10450 at 2326.088 kb on + strand, within ABZR86_RS10450 at 2326.089 kb on - strand, within ABZR86_RS10450 at 2326.636 kb on - strand, within ABZR86_RS10455 at 2326.680 kb on + strand, within ABZR86_RS10455 at 2326.776 kb on + strand, within ABZR86_RS10455 at 2326.776 kb on + strand, within ABZR86_RS10455 at 2326.777 kb on - strand, within ABZR86_RS10455 at 2326.777 kb on - strand, within ABZR86_RS10455 at 2326.777 kb on - strand, within ABZR86_RS10455 at 2326.786 kb on + strand, within ABZR86_RS10455 at 2326.786 kb on + strand, within ABZR86_RS10455 at 2326.964 kb on + strand at 2326.964 kb on + strand at 2326.965 kb on - strand at 2327.071 kb on + strand at 2327.101 kb on - strand at 2327.116 kb on + strand at 2327.117 kb on - strand at 2327.139 kb on - strand at 2327.148 kb on + strand at 2327.148 kb on + strand at 2327.149 kb on - strand at 2327.150 kb on + strand at 2327.150 kb on + strand at 2327.150 kb on + strand at 2327.150 kb on + strand at 2327.150 kb on + strand at 2327.150 kb on + strand at 2327.151 kb on - strand at 2327.151 kb on - strand at 2327.151 kb on - strand at 2327.151 kb on - strand at 2327.151 kb on - strand at 2327.356 kb on - strand at 2327.440 kb on - strand, within ABZR86_RS10460 at 2327.440 kb on - strand, within ABZR86_RS10460 at 2327.503 kb on + strand, within ABZR86_RS10460 at 2327.503 kb on + strand, within ABZR86_RS10460 at 2327.503 kb on + strand, within ABZR86_RS10460 at 2327.504 kb on - strand, within ABZR86_RS10460 at 2327.504 kb on - strand, within ABZR86_RS10460
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.4 mg/ml remove 2,324,917 + ABZR86_RS10440 0.65 -0.6 2,324,917 + ABZR86_RS10440 0.65 -0.3 2,325,016 + ABZR86_RS10440 0.88 -0.5 2,325,017 - ABZR86_RS10440 0.88 +0.4 2,325,748 + -0.1 2,325,748 + +0.1 2,325,748 + +0.7 2,325,748 + +0.3 2,325,748 + +0.6 2,325,748 + -1.0 2,325,791 + -0.1 2,325,792 - +0.6 2,325,825 - ABZR86_RS10450 0.11 +0.3 2,325,825 - ABZR86_RS10450 0.11 +0.2 2,325,941 + ABZR86_RS10450 0.25 -1.9 2,325,941 + ABZR86_RS10450 0.25 +0.3 2,325,942 - ABZR86_RS10450 0.25 -0.2 2,325,945 - ABZR86_RS10450 0.25 +0.6 2,325,945 - ABZR86_RS10450 0.25 -0.5 2,326,088 + ABZR86_RS10450 0.43 -0.7 2,326,089 - ABZR86_RS10450 0.43 -0.2 2,326,636 - ABZR86_RS10455 0.11 +0.6 2,326,680 + ABZR86_RS10455 0.25 -0.6 2,326,776 + ABZR86_RS10455 0.55 -0.5 2,326,776 + ABZR86_RS10455 0.55 +0.5 2,326,777 - ABZR86_RS10455 0.55 +0.0 2,326,777 - ABZR86_RS10455 0.55 +0.8 2,326,777 - ABZR86_RS10455 0.55 -0.1 2,326,786 + ABZR86_RS10455 0.58 -1.7 2,326,786 + ABZR86_RS10455 0.58 -0.8 2,326,964 + -1.0 2,326,964 + -0.5 2,326,965 - -0.3 2,327,071 + -1.1 2,327,101 - -0.6 2,327,116 + -1.0 2,327,117 - +0.8 2,327,139 - +0.7 2,327,148 + -1.1 2,327,148 + -1.4 2,327,149 - -0.2 2,327,150 + +0.1 2,327,150 + -0.7 2,327,150 + -2.4 2,327,150 + -0.8 2,327,150 + +0.4 2,327,150 + -1.7 2,327,151 - +0.0 2,327,151 - +0.1 2,327,151 - -1.2 2,327,151 - +0.7 2,327,151 - +0.4 2,327,356 - -0.1 2,327,440 - ABZR86_RS10460 0.20 -0.6 2,327,440 - ABZR86_RS10460 0.20 +0.5 2,327,503 + ABZR86_RS10460 0.29 -0.4 2,327,503 + ABZR86_RS10460 0.29 -0.2 2,327,503 + ABZR86_RS10460 0.29 +0.5 2,327,504 - ABZR86_RS10460 0.29 +0.3 2,327,504 - ABZR86_RS10460 0.29 +0.8
Or see this region's nucleotide sequence