Experiment: Fusidic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS10065 and ABZR86_RS10070 are separated by 139 nucleotides ABZR86_RS10070 and ABZR86_RS10075 are separated by 3 nucleotides ABZR86_RS10075 and ABZR86_RS10080 are separated by 264 nucleotides
ABZR86_RS10065: ABZR86_RS10065 - dihydrofolate reductase, at 2,234,398 to 2,234,889
_RS10065
ABZR86_RS10070: ABZR86_RS10070 - thymidylate synthase, at 2,235,029 to 2,235,823
_RS10070
ABZR86_RS10075: ABZR86_RS10075 - prolipoprotein diacylglyceryl transferase, at 2,235,827 to 2,236,657
_RS10075
ABZR86_RS10080: ABZR86_RS10080 - prolyl oligopeptidase family serine peptidase, at 2,236,922 to 2,238,892
_RS10080
Position (kb)
2235
2236
2237 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2234.968 kb on - strand at 2235.856 kb on - strand at 2236.692 kb on + strand at 2236.692 kb on + strand at 2236.692 kb on + strand at 2236.692 kb on + strand at 2236.693 kb on - strand at 2236.693 kb on - strand at 2236.731 kb on + strand at 2236.776 kb on + strand at 2236.777 kb on - strand at 2236.777 kb on - strand at 2236.777 kb on - strand at 2236.777 kb on - strand at 2236.777 kb on - strand at 2236.778 kb on + strand at 2236.778 kb on + strand at 2236.779 kb on - strand at 2236.779 kb on - strand at 2236.929 kb on - strand at 2237.066 kb on + strand at 2237.066 kb on + strand at 2237.066 kb on + strand at 2237.066 kb on + strand at 2237.066 kb on + strand at 2237.066 kb on + strand at 2237.066 kb on + strand at 2237.066 kb on + strand at 2237.067 kb on - strand at 2237.067 kb on - strand at 2237.067 kb on - strand at 2237.067 kb on - strand at 2237.067 kb on - strand at 2237.067 kb on - strand at 2237.120 kb on + strand, within ABZR86_RS10080 at 2237.120 kb on + strand, within ABZR86_RS10080 at 2237.121 kb on - strand, within ABZR86_RS10080 at 2237.121 kb on - strand, within ABZR86_RS10080 at 2237.121 kb on - strand, within ABZR86_RS10080 at 2237.171 kb on + strand, within ABZR86_RS10080 at 2237.171 kb on + strand, within ABZR86_RS10080 at 2237.171 kb on + strand, within ABZR86_RS10080 at 2237.171 kb on + strand, within ABZR86_RS10080 at 2237.172 kb on - strand, within ABZR86_RS10080 at 2237.172 kb on - strand, within ABZR86_RS10080 at 2237.172 kb on - strand, within ABZR86_RS10080 at 2237.172 kb on - strand, within ABZR86_RS10080 at 2237.172 kb on - strand, within ABZR86_RS10080 at 2237.172 kb on - strand, within ABZR86_RS10080 at 2237.309 kb on + strand, within ABZR86_RS10080 at 2237.309 kb on + strand, within ABZR86_RS10080 at 2237.315 kb on + strand, within ABZR86_RS10080 at 2237.316 kb on - strand, within ABZR86_RS10080 at 2237.316 kb on - strand, within ABZR86_RS10080 at 2237.417 kb on + strand, within ABZR86_RS10080 at 2237.417 kb on + strand, within ABZR86_RS10080 at 2237.417 kb on + strand, within ABZR86_RS10080 at 2237.417 kb on + strand, within ABZR86_RS10080 at 2237.418 kb on - strand, within ABZR86_RS10080 at 2237.462 kb on + strand, within ABZR86_RS10080 at 2237.463 kb on - strand, within ABZR86_RS10080 at 2237.463 kb on - strand, within ABZR86_RS10080 at 2237.582 kb on + strand, within ABZR86_RS10080 at 2237.582 kb on + strand, within ABZR86_RS10080 at 2237.582 kb on + strand, within ABZR86_RS10080 at 2237.582 kb on + strand, within ABZR86_RS10080 at 2237.582 kb on + strand, within ABZR86_RS10080 at 2237.583 kb on - strand, within ABZR86_RS10080 at 2237.583 kb on - strand, within ABZR86_RS10080
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.4 mg/ml remove 2,234,968 - -2.1 2,235,856 - +0.5 2,236,692 + -1.3 2,236,692 + +0.1 2,236,692 + -0.2 2,236,692 + -0.9 2,236,693 - -0.3 2,236,693 - +0.1 2,236,731 + -0.2 2,236,776 + -0.3 2,236,777 - -0.9 2,236,777 - +0.4 2,236,777 - -0.3 2,236,777 - -0.0 2,236,777 - +0.5 2,236,778 + +0.0 2,236,778 + -0.2 2,236,779 - +0.4 2,236,779 - +0.1 2,236,929 - -0.2 2,237,066 + +0.1 2,237,066 + -0.0 2,237,066 + -0.3 2,237,066 + +0.3 2,237,066 + -0.3 2,237,066 + +0.9 2,237,066 + +0.1 2,237,066 + +0.3 2,237,067 - -0.3 2,237,067 - -0.6 2,237,067 - -0.2 2,237,067 - -0.5 2,237,067 - -0.3 2,237,067 - -0.1 2,237,120 + ABZR86_RS10080 0.10 -0.1 2,237,120 + ABZR86_RS10080 0.10 +0.0 2,237,121 - ABZR86_RS10080 0.10 +0.2 2,237,121 - ABZR86_RS10080 0.10 -0.3 2,237,121 - ABZR86_RS10080 0.10 +0.3 2,237,171 + ABZR86_RS10080 0.13 -0.2 2,237,171 + ABZR86_RS10080 0.13 -0.8 2,237,171 + ABZR86_RS10080 0.13 +0.4 2,237,171 + ABZR86_RS10080 0.13 -0.4 2,237,172 - ABZR86_RS10080 0.13 +0.2 2,237,172 - ABZR86_RS10080 0.13 -0.3 2,237,172 - ABZR86_RS10080 0.13 -0.1 2,237,172 - ABZR86_RS10080 0.13 -0.4 2,237,172 - ABZR86_RS10080 0.13 -0.3 2,237,172 - ABZR86_RS10080 0.13 -0.2 2,237,309 + ABZR86_RS10080 0.20 -0.0 2,237,309 + ABZR86_RS10080 0.20 +0.4 2,237,315 + ABZR86_RS10080 0.20 -0.0 2,237,316 - ABZR86_RS10080 0.20 -0.0 2,237,316 - ABZR86_RS10080 0.20 -0.5 2,237,417 + ABZR86_RS10080 0.25 +0.6 2,237,417 + ABZR86_RS10080 0.25 +0.1 2,237,417 + ABZR86_RS10080 0.25 -1.4 2,237,417 + ABZR86_RS10080 0.25 +1.1 2,237,418 - ABZR86_RS10080 0.25 -0.1 2,237,462 + ABZR86_RS10080 0.27 +0.1 2,237,463 - ABZR86_RS10080 0.27 -0.4 2,237,463 - ABZR86_RS10080 0.27 +0.2 2,237,582 + ABZR86_RS10080 0.33 -0.1 2,237,582 + ABZR86_RS10080 0.33 -0.8 2,237,582 + ABZR86_RS10080 0.33 -0.5 2,237,582 + ABZR86_RS10080 0.33 +0.4 2,237,582 + ABZR86_RS10080 0.33 -0.1 2,237,583 - ABZR86_RS10080 0.34 -0.4 2,237,583 - ABZR86_RS10080 0.34 +2.4
Or see this region's nucleotide sequence