Experiment: Fusidic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS02980 and ABZR86_RS02985 overlap by 14 nucleotides ABZR86_RS02985 and ABZR86_RS02990 are separated by 27 nucleotides ABZR86_RS02990 and ABZR86_RS02995 are separated by 44 nucleotides
ABZR86_RS02980: ABZR86_RS02980 - DUF3182 family protein, at 609,594 to 610,724
_RS02980
ABZR86_RS02985: ABZR86_RS02985 - alpha/beta fold hydrolase, at 610,711 to 611,526
_RS02985
ABZR86_RS02990: ABZR86_RS02990 - enterotoxin A family protein, at 611,554 to 612,549
_RS02990
ABZR86_RS02995: ABZR86_RS02995 - hypothetical protein, at 612,594 to 613,526
_RS02995
Position (kb) 611
612
613 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 610.611 kb on + strand, within ABZR86_RS02980 at 610.612 kb on - strand at 610.692 kb on + strand at 610.692 kb on + strand at 610.693 kb on - strand at 610.693 kb on - strand at 610.693 kb on - strand at 610.880 kb on + strand, within ABZR86_RS02985 at 611.113 kb on + strand, within ABZR86_RS02985 at 611.302 kb on + strand, within ABZR86_RS02985 at 611.338 kb on + strand, within ABZR86_RS02985 at 611.339 kb on - strand, within ABZR86_RS02985 at 611.558 kb on - strand at 611.558 kb on - strand at 611.558 kb on - strand at 611.879 kb on + strand, within ABZR86_RS02990 at 611.879 kb on + strand, within ABZR86_RS02990 at 611.879 kb on + strand, within ABZR86_RS02990 at 611.915 kb on + strand, within ABZR86_RS02990 at 611.915 kb on + strand, within ABZR86_RS02990 at 611.915 kb on + strand, within ABZR86_RS02990 at 611.916 kb on - strand, within ABZR86_RS02990 at 611.984 kb on - strand, within ABZR86_RS02990 at 612.195 kb on + strand, within ABZR86_RS02990 at 612.203 kb on + strand, within ABZR86_RS02990 at 612.231 kb on - strand, within ABZR86_RS02990 at 612.236 kb on + strand, within ABZR86_RS02990 at 612.236 kb on + strand, within ABZR86_RS02990 at 612.236 kb on + strand, within ABZR86_RS02990 at 612.236 kb on + strand, within ABZR86_RS02990 at 612.237 kb on - strand, within ABZR86_RS02990 at 612.237 kb on - strand, within ABZR86_RS02990 at 612.239 kb on - strand, within ABZR86_RS02990 at 612.356 kb on - strand, within ABZR86_RS02990 at 612.371 kb on + strand, within ABZR86_RS02990 at 612.371 kb on + strand, within ABZR86_RS02990 at 612.525 kb on - strand at 612.528 kb on + strand at 612.528 kb on + strand at 612.684 kb on - strand at 612.768 kb on + strand, within ABZR86_RS02995 at 612.941 kb on - strand, within ABZR86_RS02995 at 613.034 kb on + strand, within ABZR86_RS02995 at 613.034 kb on + strand, within ABZR86_RS02995 at 613.035 kb on - strand, within ABZR86_RS02995 at 613.083 kb on + strand, within ABZR86_RS02995 at 613.087 kb on + strand, within ABZR86_RS02995 at 613.088 kb on - strand, within ABZR86_RS02995 at 613.088 kb on - strand, within ABZR86_RS02995 at 613.232 kb on - strand, within ABZR86_RS02995 at 613.237 kb on + strand, within ABZR86_RS02995 at 613.237 kb on + strand, within ABZR86_RS02995 at 613.246 kb on + strand, within ABZR86_RS02995 at 613.246 kb on + strand, within ABZR86_RS02995 at 613.247 kb on - strand, within ABZR86_RS02995 at 613.250 kb on + strand, within ABZR86_RS02995 at 613.251 kb on - strand, within ABZR86_RS02995 at 613.306 kb on - strand, within ABZR86_RS02995 at 613.407 kb on + strand, within ABZR86_RS02995 at 613.435 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.4 mg/ml remove 610,611 + ABZR86_RS02980 0.90 -0.1 610,612 - +1.0 610,692 + -0.7 610,692 + +0.4 610,693 - -0.2 610,693 - +1.4 610,693 - +1.3 610,880 + ABZR86_RS02985 0.21 +0.6 611,113 + ABZR86_RS02985 0.49 +1.1 611,302 + ABZR86_RS02985 0.72 +0.3 611,338 + ABZR86_RS02985 0.77 -0.9 611,339 - ABZR86_RS02985 0.77 +0.3 611,558 - +0.1 611,558 - -0.5 611,558 - -0.5 611,879 + ABZR86_RS02990 0.33 -0.5 611,879 + ABZR86_RS02990 0.33 +0.9 611,879 + ABZR86_RS02990 0.33 -0.2 611,915 + ABZR86_RS02990 0.36 +0.1 611,915 + ABZR86_RS02990 0.36 +0.1 611,915 + ABZR86_RS02990 0.36 +0.3 611,916 - ABZR86_RS02990 0.36 +0.7 611,984 - ABZR86_RS02990 0.43 +1.5 612,195 + ABZR86_RS02990 0.64 +0.5 612,203 + ABZR86_RS02990 0.65 -0.4 612,231 - ABZR86_RS02990 0.68 +0.4 612,236 + ABZR86_RS02990 0.68 +0.5 612,236 + ABZR86_RS02990 0.68 +0.1 612,236 + ABZR86_RS02990 0.68 -0.3 612,236 + ABZR86_RS02990 0.68 -1.1 612,237 - ABZR86_RS02990 0.69 -0.2 612,237 - ABZR86_RS02990 0.69 -1.4 612,239 - ABZR86_RS02990 0.69 -0.1 612,356 - ABZR86_RS02990 0.81 +0.8 612,371 + ABZR86_RS02990 0.82 +0.1 612,371 + ABZR86_RS02990 0.82 +0.0 612,525 - +0.6 612,528 + -0.2 612,528 + -1.2 612,684 - +0.1 612,768 + ABZR86_RS02995 0.19 +0.1 612,941 - ABZR86_RS02995 0.37 -1.2 613,034 + ABZR86_RS02995 0.47 +0.1 613,034 + ABZR86_RS02995 0.47 -0.3 613,035 - ABZR86_RS02995 0.47 -0.3 613,083 + ABZR86_RS02995 0.52 -3.3 613,087 + ABZR86_RS02995 0.53 +2.1 613,088 - ABZR86_RS02995 0.53 -0.2 613,088 - ABZR86_RS02995 0.53 -0.7 613,232 - ABZR86_RS02995 0.68 +0.4 613,237 + ABZR86_RS02995 0.69 -1.2 613,237 + ABZR86_RS02995 0.69 -0.0 613,246 + ABZR86_RS02995 0.70 -1.6 613,246 + ABZR86_RS02995 0.70 +0.4 613,247 - ABZR86_RS02995 0.70 -0.4 613,250 + ABZR86_RS02995 0.70 +0.8 613,251 - ABZR86_RS02995 0.70 -0.9 613,306 - ABZR86_RS02995 0.76 -0.1 613,407 + ABZR86_RS02995 0.87 +0.2 613,435 + +0.7
Or see this region's nucleotide sequence