Experiment: MoYLS4 with Dimethyl Sulfoxide 7.5 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvC and DvMF_3203 are separated by 331 nucleotides DvMF_3203 and DvMF_3204 are separated by 241 nucleotides DvMF_3204 and ruvB overlap by 1 nucleotides
DvMF_3202: ruvC - Holliday junction resolvase (RefSeq), at 4,034,295 to 4,034,888
ruvC
DvMF_3203: DvMF_3203 - diguanylate cyclase (RefSeq), at 4,035,220 to 4,036,929
_3203
DvMF_3204: DvMF_3204 - Holliday junction DNA helicase RuvA (RefSeq), at 4,037,171 to 4,037,779
_3204
DvMF_3205: ruvB - Holliday junction DNA helicase RuvB (RefSeq), at 4,037,779 to 4,038,783
ruvB
Position (kb)
4035
4036
4037 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4034.484 kb on - strand, within ruvC at 4034.509 kb on - strand, within ruvC at 4034.691 kb on - strand, within ruvC at 4034.772 kb on - strand, within ruvC at 4034.921 kb on + strand at 4034.929 kb on - strand at 4034.995 kb on + strand at 4035.246 kb on + strand at 4035.246 kb on + strand at 4035.246 kb on + strand at 4035.327 kb on - strand at 4035.338 kb on + strand at 4035.501 kb on - strand, within DvMF_3203 at 4035.661 kb on - strand, within DvMF_3203 at 4035.671 kb on - strand, within DvMF_3203 at 4035.710 kb on + strand, within DvMF_3203 at 4035.869 kb on + strand, within DvMF_3203 at 4035.869 kb on + strand, within DvMF_3203 at 4035.875 kb on - strand, within DvMF_3203 at 4035.959 kb on - strand, within DvMF_3203 at 4036.154 kb on + strand, within DvMF_3203 at 4036.232 kb on - strand, within DvMF_3203 at 4036.318 kb on - strand, within DvMF_3203 at 4036.351 kb on + strand, within DvMF_3203 at 4036.531 kb on + strand, within DvMF_3203 at 4036.728 kb on + strand, within DvMF_3203 at 4036.809 kb on - strand at 4036.834 kb on - strand at 4036.860 kb on + strand at 4037.002 kb on + strand at 4037.047 kb on - strand at 4037.083 kb on + strand at 4037.098 kb on + strand at 4037.343 kb on + strand, within DvMF_3204 at 4037.351 kb on - strand, within DvMF_3204 at 4037.385 kb on + strand, within DvMF_3204 at 4037.388 kb on + strand, within DvMF_3204 at 4037.401 kb on - strand, within DvMF_3204 at 4037.729 kb on - strand at 4037.790 kb on + strand at 4037.811 kb on + strand at 4037.872 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Dimethyl Sulfoxide 7.5 % remove 4,034,484 - ruvC DvMF_3202 0.32 -1.9 4,034,509 - ruvC DvMF_3202 0.36 -1.7 4,034,691 - ruvC DvMF_3202 0.67 -2.1 4,034,772 - ruvC DvMF_3202 0.80 -1.7 4,034,921 + -0.5 4,034,929 - -0.4 4,034,995 + -0.2 4,035,246 + -0.5 4,035,246 + -0.9 4,035,246 + -0.5 4,035,327 - -0.6 4,035,338 + -0.1 4,035,501 - DvMF_3203 0.16 -0.1 4,035,661 - DvMF_3203 0.26 -0.3 4,035,671 - DvMF_3203 0.26 -0.1 4,035,710 + DvMF_3203 0.29 +0.4 4,035,869 + DvMF_3203 0.38 -0.3 4,035,869 + DvMF_3203 0.38 -0.2 4,035,875 - DvMF_3203 0.38 -2.3 4,035,959 - DvMF_3203 0.43 -0.7 4,036,154 + DvMF_3203 0.55 -0.3 4,036,232 - DvMF_3203 0.59 -0.1 4,036,318 - DvMF_3203 0.64 -0.4 4,036,351 + DvMF_3203 0.66 -0.5 4,036,531 + DvMF_3203 0.77 -0.1 4,036,728 + DvMF_3203 0.88 -1.1 4,036,809 - -0.8 4,036,834 - -0.8 4,036,860 + -1.5 4,037,002 + -0.2 4,037,047 - +0.1 4,037,083 + +0.2 4,037,098 + -0.3 4,037,343 + DvMF_3204 0.28 -1.6 4,037,351 - DvMF_3204 0.30 -1.3 4,037,385 + DvMF_3204 0.35 -1.4 4,037,388 + DvMF_3204 0.36 -1.7 4,037,401 - DvMF_3204 0.38 -2.8 4,037,729 - -2.6 4,037,790 + +0.7 4,037,811 + -2.4 4,037,872 - -1.8
Or see this region's nucleotide sequence