Experiment: Chloramphenicol 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5925 and Pf6N2E2_5926 are separated by 45 nucleotides Pf6N2E2_5926 and Pf6N2E2_5927 are separated by 136 nucleotides Pf6N2E2_5927 and Pf6N2E2_5928 overlap by 4 nucleotides
Pf6N2E2_5925 - Transglutaminase-like enzymes, putative cysteine proteases, at 6,745,784 to 6,746,536
_5925
Pf6N2E2_5926 - Uncharacterized glutathione S-transferase-like protein, at 6,746,582 to 6,747,205
_5926
Pf6N2E2_5927 - ABC-type multidrug transport system, ATPase component, at 6,747,342 to 6,748,274
_5927
Pf6N2E2_5928 - ABC-type multidrug transport system, permease component, at 6,748,271 to 6,749,056
_5928
Position (kb)
6747
6748
6749 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6746.396 kb on + strand, within Pf6N2E2_5925 at 6746.396 kb on + strand, within Pf6N2E2_5925 at 6746.397 kb on - strand, within Pf6N2E2_5925 at 6746.397 kb on - strand, within Pf6N2E2_5925 at 6746.397 kb on - strand, within Pf6N2E2_5925 at 6746.466 kb on - strand at 6746.544 kb on + strand at 6746.544 kb on + strand at 6746.544 kb on + strand at 6746.544 kb on + strand at 6746.545 kb on - strand at 6746.561 kb on + strand at 6746.561 kb on + strand at 6746.562 kb on - strand at 6746.660 kb on + strand, within Pf6N2E2_5926 at 6746.844 kb on + strand, within Pf6N2E2_5926 at 6747.035 kb on + strand, within Pf6N2E2_5926 at 6747.036 kb on - strand, within Pf6N2E2_5926 at 6747.036 kb on - strand, within Pf6N2E2_5926 at 6747.089 kb on + strand, within Pf6N2E2_5926 at 6747.090 kb on - strand, within Pf6N2E2_5926 at 6747.090 kb on - strand, within Pf6N2E2_5926 at 6747.090 kb on - strand, within Pf6N2E2_5926 at 6747.090 kb on - strand, within Pf6N2E2_5926 at 6747.127 kb on - strand, within Pf6N2E2_5926 at 6747.127 kb on - strand, within Pf6N2E2_5926 at 6747.147 kb on - strand at 6747.147 kb on - strand at 6747.203 kb on + strand at 6747.203 kb on + strand at 6747.203 kb on + strand at 6747.203 kb on + strand at 6747.203 kb on + strand at 6747.204 kb on - strand at 6747.204 kb on - strand at 6747.206 kb on + strand at 6747.206 kb on + strand at 6747.206 kb on + strand at 6747.206 kb on + strand at 6747.206 kb on + strand at 6747.207 kb on - strand at 6747.207 kb on - strand at 6747.207 kb on - strand at 6747.207 kb on - strand at 6747.207 kb on - strand at 6747.210 kb on + strand at 6747.210 kb on + strand at 6747.210 kb on + strand at 6747.210 kb on + strand at 6747.210 kb on + strand at 6747.210 kb on + strand at 6747.211 kb on - strand at 6747.211 kb on - strand at 6747.211 kb on - strand at 6747.214 kb on - strand at 6747.231 kb on + strand at 6747.231 kb on + strand at 6747.231 kb on + strand at 6747.231 kb on + strand at 6747.232 kb on - strand at 6747.232 kb on - strand at 6747.232 kb on - strand at 6747.232 kb on - strand at 6747.299 kb on + strand at 6747.300 kb on - strand at 6747.300 kb on - strand at 6747.300 kb on - strand at 6747.300 kb on - strand at 6747.303 kb on + strand at 6747.334 kb on - strand at 6747.657 kb on + strand, within Pf6N2E2_5927 at 6747.658 kb on - strand, within Pf6N2E2_5927 at 6747.658 kb on - strand, within Pf6N2E2_5927 at 6747.658 kb on - strand, within Pf6N2E2_5927 at 6747.700 kb on - strand, within Pf6N2E2_5927 at 6747.700 kb on - strand, within Pf6N2E2_5927 at 6747.925 kb on - strand, within Pf6N2E2_5927 at 6748.130 kb on - strand, within Pf6N2E2_5927 at 6748.146 kb on - strand, within Pf6N2E2_5927 at 6748.271 kb on - strand at 6748.315 kb on + strand at 6748.316 kb on - strand at 6748.397 kb on + strand, within Pf6N2E2_5928 at 6748.397 kb on + strand, within Pf6N2E2_5928 at 6748.397 kb on + strand, within Pf6N2E2_5928 at 6748.398 kb on - strand, within Pf6N2E2_5928 at 6748.457 kb on + strand, within Pf6N2E2_5928 at 6748.457 kb on + strand, within Pf6N2E2_5928 at 6748.457 kb on + strand, within Pf6N2E2_5928 at 6748.457 kb on + strand, within Pf6N2E2_5928 at 6748.458 kb on - strand, within Pf6N2E2_5928 at 6748.458 kb on - strand, within Pf6N2E2_5928 at 6748.458 kb on - strand, within Pf6N2E2_5928 at 6748.466 kb on + strand, within Pf6N2E2_5928 at 6748.466 kb on + strand, within Pf6N2E2_5928 at 6748.466 kb on + strand, within Pf6N2E2_5928 at 6748.466 kb on + strand, within Pf6N2E2_5928 at 6748.466 kb on + strand, within Pf6N2E2_5928 at 6748.467 kb on - strand, within Pf6N2E2_5928 at 6748.467 kb on - strand, within Pf6N2E2_5928 at 6748.467 kb on - strand, within Pf6N2E2_5928 at 6748.467 kb on - strand, within Pf6N2E2_5928 at 6748.467 kb on - strand, within Pf6N2E2_5928 at 6748.467 kb on - strand, within Pf6N2E2_5928 at 6748.467 kb on - strand, within Pf6N2E2_5928 at 6748.594 kb on + strand, within Pf6N2E2_5928 at 6748.595 kb on - strand, within Pf6N2E2_5928 at 6748.610 kb on + strand, within Pf6N2E2_5928 at 6748.832 kb on + strand, within Pf6N2E2_5928 at 6748.833 kb on - strand, within Pf6N2E2_5928 at 6748.833 kb on - strand, within Pf6N2E2_5928 at 6748.836 kb on + strand, within Pf6N2E2_5928 at 6748.850 kb on + strand, within Pf6N2E2_5928 at 6748.850 kb on + strand, within Pf6N2E2_5928 at 6748.850 kb on + strand, within Pf6N2E2_5928 at 6748.932 kb on - strand, within Pf6N2E2_5928 at 6749.006 kb on + strand at 6749.006 kb on + strand at 6749.006 kb on + strand at 6749.006 kb on + strand at 6749.007 kb on - strand at 6749.055 kb on - strand at 6749.106 kb on + strand at 6749.107 kb on - strand at 6749.107 kb on - strand at 6749.228 kb on + strand at 6749.228 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.008 mg/ml remove 6,746,396 + Pf6N2E2_5925 0.81 +0.7 6,746,396 + Pf6N2E2_5925 0.81 +0.0 6,746,397 - Pf6N2E2_5925 0.81 +0.1 6,746,397 - Pf6N2E2_5925 0.81 +0.1 6,746,397 - Pf6N2E2_5925 0.81 +0.8 6,746,466 - -0.6 6,746,544 + -0.3 6,746,544 + +0.2 6,746,544 + +0.5 6,746,544 + +0.3 6,746,545 - -0.1 6,746,561 + -0.1 6,746,561 + -0.5 6,746,562 - -0.0 6,746,660 + Pf6N2E2_5926 0.12 -0.4 6,746,844 + Pf6N2E2_5926 0.42 -0.1 6,747,035 + Pf6N2E2_5926 0.73 -0.7 6,747,036 - Pf6N2E2_5926 0.73 +1.2 6,747,036 - Pf6N2E2_5926 0.73 +0.8 6,747,089 + Pf6N2E2_5926 0.81 +0.5 6,747,090 - Pf6N2E2_5926 0.81 -0.3 6,747,090 - Pf6N2E2_5926 0.81 -0.6 6,747,090 - Pf6N2E2_5926 0.81 -0.4 6,747,090 - Pf6N2E2_5926 0.81 +0.1 6,747,127 - Pf6N2E2_5926 0.87 -0.1 6,747,127 - Pf6N2E2_5926 0.87 -2.3 6,747,147 - +0.7 6,747,147 - -0.1 6,747,203 + +0.9 6,747,203 + -1.3 6,747,203 + -1.3 6,747,203 + -1.8 6,747,203 + +0.7 6,747,204 - +1.1 6,747,204 - +0.7 6,747,206 + -1.6 6,747,206 + +0.1 6,747,206 + +1.1 6,747,206 + +1.0 6,747,206 + -0.7 6,747,207 - +0.4 6,747,207 - +0.0 6,747,207 - +0.3 6,747,207 - +0.2 6,747,207 - +1.2 6,747,210 + +0.0 6,747,210 + +0.3 6,747,210 + -0.1 6,747,210 + +0.5 6,747,210 + -0.7 6,747,210 + -0.1 6,747,211 - -0.4 6,747,211 - +0.4 6,747,211 - +0.1 6,747,214 - +0.9 6,747,231 + -1.0 6,747,231 + +0.4 6,747,231 + -0.2 6,747,231 + +0.4 6,747,232 - +0.4 6,747,232 - +0.1 6,747,232 - +0.9 6,747,232 - +0.3 6,747,299 + -2.6 6,747,300 - +1.5 6,747,300 - +1.8 6,747,300 - +1.1 6,747,300 - +0.4 6,747,303 + +0.3 6,747,334 - +1.8 6,747,657 + Pf6N2E2_5927 0.34 +1.5 6,747,658 - Pf6N2E2_5927 0.34 +1.4 6,747,658 - Pf6N2E2_5927 0.34 +2.0 6,747,658 - Pf6N2E2_5927 0.34 +2.4 6,747,700 - Pf6N2E2_5927 0.38 +1.6 6,747,700 - Pf6N2E2_5927 0.38 +1.2 6,747,925 - Pf6N2E2_5927 0.62 +1.9 6,748,130 - Pf6N2E2_5927 0.84 +2.0 6,748,146 - Pf6N2E2_5927 0.86 +1.0 6,748,271 - +1.7 6,748,315 + +0.4 6,748,316 - +2.3 6,748,397 + Pf6N2E2_5928 0.16 +0.4 6,748,397 + Pf6N2E2_5928 0.16 +0.4 6,748,397 + Pf6N2E2_5928 0.16 +1.3 6,748,398 - Pf6N2E2_5928 0.16 +1.5 6,748,457 + Pf6N2E2_5928 0.24 +1.7 6,748,457 + Pf6N2E2_5928 0.24 +1.6 6,748,457 + Pf6N2E2_5928 0.24 +2.0 6,748,457 + Pf6N2E2_5928 0.24 +1.6 6,748,458 - Pf6N2E2_5928 0.24 +1.7 6,748,458 - Pf6N2E2_5928 0.24 +1.5 6,748,458 - Pf6N2E2_5928 0.24 +1.5 6,748,466 + Pf6N2E2_5928 0.25 +1.5 6,748,466 + Pf6N2E2_5928 0.25 +0.9 6,748,466 + Pf6N2E2_5928 0.25 +2.5 6,748,466 + Pf6N2E2_5928 0.25 +1.5 6,748,466 + Pf6N2E2_5928 0.25 +1.8 6,748,467 - Pf6N2E2_5928 0.25 +2.6 6,748,467 - Pf6N2E2_5928 0.25 +1.5 6,748,467 - Pf6N2E2_5928 0.25 +1.3 6,748,467 - Pf6N2E2_5928 0.25 +2.0 6,748,467 - Pf6N2E2_5928 0.25 +1.5 6,748,467 - Pf6N2E2_5928 0.25 +1.8 6,748,467 - Pf6N2E2_5928 0.25 +1.1 6,748,594 + Pf6N2E2_5928 0.41 +2.0 6,748,595 - Pf6N2E2_5928 0.41 +1.9 6,748,610 + Pf6N2E2_5928 0.43 +1.7 6,748,832 + Pf6N2E2_5928 0.71 +2.0 6,748,833 - Pf6N2E2_5928 0.72 +1.6 6,748,833 - Pf6N2E2_5928 0.72 +1.8 6,748,836 + Pf6N2E2_5928 0.72 +1.4 6,748,850 + Pf6N2E2_5928 0.74 +1.8 6,748,850 + Pf6N2E2_5928 0.74 +2.0 6,748,850 + Pf6N2E2_5928 0.74 +2.6 6,748,932 - Pf6N2E2_5928 0.84 +1.5 6,749,006 + +2.8 6,749,006 + +2.1 6,749,006 + +1.1 6,749,006 + +2.6 6,749,007 - +2.1 6,749,055 - +2.8 6,749,106 + +0.1 6,749,107 - -0.8 6,749,107 - +0.2 6,749,228 + +0.9 6,749,228 + -1.8
Or see this region's nucleotide sequence