Experiment: Chloramphenicol 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_555 and Pf6N2E2_556 are separated by 83 nucleotides Pf6N2E2_556 and Pf6N2E2_557 are separated by 24 nucleotides Pf6N2E2_557 and Pf6N2E2_558 are separated by 63 nucleotides Pf6N2E2_558 and Pf6N2E2_559 are separated by 18 nucleotides Pf6N2E2_559 and Pf6N2E2_560 overlap by 4 nucleotides
Pf6N2E2_555 - Uncharacterized protein ImpB, at 627,315 to 627,875
_555
Pf6N2E2_556 - hypothetical protein, at 627,959 to 628,075
_556
Pf6N2E2_557 - Uncharacterized protein homologous to Paeru. PA2364, at 628,100 to 628,549
_557
Pf6N2E2_558 - Uncharacterized protein ImpJ/VasE, at 628,613 to 629,944
_558
Pf6N2E2_559 - Inner membrane protein DotU, at 629,963 to 630,727
_559
Pf6N2E2_560 - IcmF-related protein, at 630,724 to 634,548
_560
Position (kb) 628
629
630 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 627.814 kb on + strand, within Pf6N2E2_555 at 627.814 kb on + strand at 627.814 kb on + strand, within Pf6N2E2_555 at 627.814 kb on + strand, within Pf6N2E2_555 at 627.815 kb on - strand, within Pf6N2E2_555 at 627.815 kb on - strand, within Pf6N2E2_555 at 627.864 kb on - strand at 628.023 kb on + strand, within Pf6N2E2_556 at 628.036 kb on - strand, within Pf6N2E2_556 at 628.445 kb on + strand, within Pf6N2E2_557 at 628.445 kb on + strand, within Pf6N2E2_557 at 628.445 kb on + strand, within Pf6N2E2_557 at 628.445 kb on + strand, within Pf6N2E2_557 at 628.445 kb on + strand, within Pf6N2E2_557 at 628.445 kb on + strand, within Pf6N2E2_557 at 628.445 kb on + strand, within Pf6N2E2_557 at 628.445 kb on + strand, within Pf6N2E2_557 at 628.446 kb on - strand, within Pf6N2E2_557 at 628.446 kb on - strand, within Pf6N2E2_557 at 628.446 kb on - strand, within Pf6N2E2_557 at 628.446 kb on - strand, within Pf6N2E2_557 at 628.448 kb on - strand, within Pf6N2E2_557 at 628.448 kb on - strand, within Pf6N2E2_557 at 628.448 kb on - strand, within Pf6N2E2_557 at 628.612 kb on + strand at 628.613 kb on - strand at 628.925 kb on + strand, within Pf6N2E2_558 at 628.925 kb on + strand, within Pf6N2E2_558 at 628.925 kb on + strand, within Pf6N2E2_558 at 628.926 kb on - strand, within Pf6N2E2_558 at 629.156 kb on + strand, within Pf6N2E2_558 at 629.393 kb on + strand, within Pf6N2E2_558 at 629.394 kb on - strand, within Pf6N2E2_558 at 629.786 kb on + strand, within Pf6N2E2_558 at 629.786 kb on + strand, within Pf6N2E2_558 at 629.787 kb on - strand, within Pf6N2E2_558 at 629.962 kb on + strand at 629.962 kb on + strand at 629.962 kb on + strand at 629.962 kb on + strand at 629.962 kb on + strand at 629.962 kb on + strand at 629.962 kb on + strand at 629.962 kb on + strand at 629.963 kb on - strand at 630.090 kb on + strand, within Pf6N2E2_559 at 630.201 kb on - strand, within Pf6N2E2_559 at 630.201 kb on - strand, within Pf6N2E2_559 at 630.293 kb on + strand, within Pf6N2E2_559 at 630.293 kb on + strand, within Pf6N2E2_559 at 630.293 kb on + strand, within Pf6N2E2_559 at 630.293 kb on + strand, within Pf6N2E2_559 at 630.293 kb on + strand, within Pf6N2E2_559 at 630.294 kb on - strand, within Pf6N2E2_559 at 630.294 kb on - strand, within Pf6N2E2_559 at 630.294 kb on - strand, within Pf6N2E2_559 at 630.294 kb on - strand, within Pf6N2E2_559 at 630.294 kb on - strand, within Pf6N2E2_559 at 630.294 kb on - strand, within Pf6N2E2_559 at 630.294 kb on - strand, within Pf6N2E2_559 at 630.294 kb on - strand, within Pf6N2E2_559 at 630.343 kb on - strand, within Pf6N2E2_559 at 630.387 kb on - strand, within Pf6N2E2_559 at 630.503 kb on + strand, within Pf6N2E2_559 at 630.503 kb on + strand, within Pf6N2E2_559 at 630.503 kb on + strand, within Pf6N2E2_559 at 630.839 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.008 mg/ml remove 627,814 + Pf6N2E2_555 0.89 -1.0 627,814 + +2.9 627,814 + Pf6N2E2_555 0.89 +0.5 627,814 + Pf6N2E2_555 0.89 +0.4 627,815 - Pf6N2E2_555 0.89 +1.6 627,815 - Pf6N2E2_555 0.89 -0.0 627,864 - -0.1 628,023 + Pf6N2E2_556 0.55 +0.4 628,036 - Pf6N2E2_556 0.66 +0.5 628,445 + Pf6N2E2_557 0.77 +0.5 628,445 + Pf6N2E2_557 0.77 -0.3 628,445 + Pf6N2E2_557 0.77 -1.3 628,445 + Pf6N2E2_557 0.77 +0.3 628,445 + Pf6N2E2_557 0.77 -0.7 628,445 + Pf6N2E2_557 0.77 +0.5 628,445 + Pf6N2E2_557 0.77 -0.9 628,445 + Pf6N2E2_557 0.77 -1.6 628,446 - Pf6N2E2_557 0.77 +0.6 628,446 - Pf6N2E2_557 0.77 -0.9 628,446 - Pf6N2E2_557 0.77 -1.2 628,446 - Pf6N2E2_557 0.77 +0.2 628,448 - Pf6N2E2_557 0.77 +0.5 628,448 - Pf6N2E2_557 0.77 -0.8 628,448 - Pf6N2E2_557 0.77 -0.1 628,612 + -0.1 628,613 - +0.7 628,925 + Pf6N2E2_558 0.23 -0.0 628,925 + Pf6N2E2_558 0.23 +0.3 628,925 + Pf6N2E2_558 0.23 -1.9 628,926 - Pf6N2E2_558 0.23 -0.0 629,156 + Pf6N2E2_558 0.41 +0.6 629,393 + Pf6N2E2_558 0.59 -0.4 629,394 - Pf6N2E2_558 0.59 -0.6 629,786 + Pf6N2E2_558 0.88 -0.3 629,786 + Pf6N2E2_558 0.88 -0.8 629,787 - Pf6N2E2_558 0.88 -1.6 629,962 + +1.2 629,962 + +0.4 629,962 + +0.7 629,962 + -0.7 629,962 + +0.6 629,962 + -0.7 629,962 + -0.6 629,962 + -1.3 629,963 - +0.1 630,090 + Pf6N2E2_559 0.17 -0.1 630,201 - Pf6N2E2_559 0.31 -0.7 630,201 - Pf6N2E2_559 0.31 -0.1 630,293 + Pf6N2E2_559 0.43 -0.4 630,293 + Pf6N2E2_559 0.43 +0.3 630,293 + Pf6N2E2_559 0.43 -0.3 630,293 + Pf6N2E2_559 0.43 -0.3 630,293 + Pf6N2E2_559 0.43 +0.7 630,294 - Pf6N2E2_559 0.43 -0.4 630,294 - Pf6N2E2_559 0.43 +0.0 630,294 - Pf6N2E2_559 0.43 -0.5 630,294 - Pf6N2E2_559 0.43 -0.5 630,294 - Pf6N2E2_559 0.43 +0.3 630,294 - Pf6N2E2_559 0.43 -0.1 630,294 - Pf6N2E2_559 0.43 -0.7 630,294 - Pf6N2E2_559 0.43 -0.9 630,343 - Pf6N2E2_559 0.50 +1.3 630,387 - Pf6N2E2_559 0.55 -1.6 630,503 + Pf6N2E2_559 0.71 +1.1 630,503 + Pf6N2E2_559 0.71 -0.2 630,503 + Pf6N2E2_559 0.71 -0.8 630,839 - +1.0
Or see this region's nucleotide sequence