Experiment: Chloramphenicol 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4763 and Pf6N2E2_4764 overlap by 4 nucleotides Pf6N2E2_4764 and Pf6N2E2_4765 are separated by 118 nucleotides Pf6N2E2_4765 and Pf6N2E2_4766 are separated by 276 nucleotides
Pf6N2E2_4763 - Biotin synthesis protein BioC, at 5,501,269 to 5,502,081
_4763
Pf6N2E2_4764 - Dethiobiotin synthetase (EC 6.3.3.3), at 5,502,078 to 5,502,758
_4764
Pf6N2E2_4765 - FIG00954771: hypothetical protein, at 5,502,877 to 5,503,173
_4765
Pf6N2E2_4766 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 5,503,450 to 5,505,255
_4766
Position (kb)
5502
5503
5504 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5501.888 kb on - strand, within Pf6N2E2_4763 at 5502.004 kb on + strand at 5502.004 kb on + strand at 5502.004 kb on + strand at 5502.004 kb on + strand at 5502.005 kb on - strand at 5502.005 kb on - strand at 5502.043 kb on + strand at 5502.043 kb on + strand at 5502.043 kb on + strand at 5502.043 kb on + strand at 5502.043 kb on + strand at 5502.044 kb on - strand at 5502.044 kb on - strand at 5502.078 kb on - strand at 5502.090 kb on + strand at 5502.091 kb on - strand at 5502.282 kb on + strand, within Pf6N2E2_4764 at 5502.282 kb on + strand, within Pf6N2E2_4764 at 5502.282 kb on + strand, within Pf6N2E2_4764 at 5502.282 kb on + strand, within Pf6N2E2_4764 at 5502.282 kb on + strand, within Pf6N2E2_4764 at 5502.282 kb on + strand, within Pf6N2E2_4764 at 5502.282 kb on + strand, within Pf6N2E2_4764 at 5502.282 kb on + strand, within Pf6N2E2_4764 at 5502.283 kb on - strand, within Pf6N2E2_4764 at 5502.283 kb on - strand, within Pf6N2E2_4764 at 5502.283 kb on - strand, within Pf6N2E2_4764 at 5502.283 kb on - strand, within Pf6N2E2_4764 at 5502.283 kb on - strand, within Pf6N2E2_4764 at 5502.894 kb on + strand at 5502.904 kb on + strand at 5502.904 kb on + strand at 5502.904 kb on + strand at 5502.904 kb on + strand at 5502.905 kb on - strand at 5502.905 kb on - strand at 5502.905 kb on - strand at 5502.905 kb on - strand at 5502.905 kb on - strand at 5502.975 kb on + strand, within Pf6N2E2_4765 at 5502.976 kb on - strand, within Pf6N2E2_4765 at 5503.116 kb on + strand, within Pf6N2E2_4765 at 5503.116 kb on + strand, within Pf6N2E2_4765 at 5503.116 kb on + strand, within Pf6N2E2_4765 at 5503.117 kb on - strand, within Pf6N2E2_4765 at 5503.117 kb on - strand, within Pf6N2E2_4765 at 5503.117 kb on - strand, within Pf6N2E2_4765 at 5503.149 kb on + strand at 5503.150 kb on - strand at 5503.161 kb on + strand at 5503.161 kb on + strand at 5503.162 kb on - strand at 5503.236 kb on + strand at 5503.236 kb on + strand at 5503.237 kb on - strand at 5503.237 kb on - strand at 5503.237 kb on - strand at 5503.237 kb on - strand at 5503.237 kb on - strand at 5503.286 kb on + strand at 5503.286 kb on + strand at 5503.286 kb on + strand at 5503.368 kb on + strand at 5503.368 kb on + strand at 5503.368 kb on + strand at 5503.368 kb on + strand at 5503.369 kb on - strand at 5503.369 kb on - strand at 5503.394 kb on + strand at 5503.395 kb on - strand at 5503.427 kb on + strand at 5503.428 kb on - strand at 5503.428 kb on - strand at 5503.444 kb on - strand at 5503.449 kb on + strand at 5503.460 kb on - strand at 5503.460 kb on - strand at 5503.460 kb on - strand at 5503.460 kb on - strand at 5503.460 kb on - strand at 5503.460 kb on - strand at 5503.522 kb on + strand at 5503.523 kb on - strand at 5503.696 kb on + strand, within Pf6N2E2_4766 at 5503.696 kb on + strand, within Pf6N2E2_4766 at 5503.696 kb on + strand, within Pf6N2E2_4766 at 5503.696 kb on + strand, within Pf6N2E2_4766 at 5503.697 kb on - strand, within Pf6N2E2_4766 at 5503.697 kb on - strand, within Pf6N2E2_4766 at 5503.697 kb on - strand, within Pf6N2E2_4766 at 5503.697 kb on - strand, within Pf6N2E2_4766 at 5503.828 kb on + strand, within Pf6N2E2_4766 at 5503.829 kb on - strand, within Pf6N2E2_4766 at 5503.897 kb on + strand, within Pf6N2E2_4766 at 5503.897 kb on + strand, within Pf6N2E2_4766 at 5503.897 kb on + strand, within Pf6N2E2_4766 at 5503.897 kb on + strand, within Pf6N2E2_4766 at 5503.897 kb on + strand, within Pf6N2E2_4766 at 5504.014 kb on + strand, within Pf6N2E2_4766 at 5504.014 kb on + strand, within Pf6N2E2_4766 at 5504.014 kb on + strand, within Pf6N2E2_4766 at 5504.014 kb on + strand, within Pf6N2E2_4766 at 5504.014 kb on + strand, within Pf6N2E2_4766 at 5504.014 kb on + strand, within Pf6N2E2_4766 at 5504.014 kb on + strand, within Pf6N2E2_4766 at 5504.015 kb on - strand, within Pf6N2E2_4766 at 5504.015 kb on - strand, within Pf6N2E2_4766 at 5504.015 kb on - strand, within Pf6N2E2_4766 at 5504.015 kb on - strand, within Pf6N2E2_4766 at 5504.119 kb on - strand, within Pf6N2E2_4766
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.008 mg/ml remove 5,501,888 - Pf6N2E2_4763 0.76 +0.4 5,502,004 + -0.7 5,502,004 + -0.1 5,502,004 + -0.9 5,502,004 + -0.1 5,502,005 - +0.8 5,502,005 - -0.5 5,502,043 + +0.7 5,502,043 + -0.2 5,502,043 + -0.4 5,502,043 + -0.9 5,502,043 + +0.4 5,502,044 - -0.3 5,502,044 - +1.1 5,502,078 - -0.4 5,502,090 + +0.3 5,502,091 - +0.9 5,502,282 + Pf6N2E2_4764 0.30 -1.4 5,502,282 + Pf6N2E2_4764 0.30 +0.3 5,502,282 + Pf6N2E2_4764 0.30 -1.5 5,502,282 + Pf6N2E2_4764 0.30 -0.3 5,502,282 + Pf6N2E2_4764 0.30 -1.4 5,502,282 + Pf6N2E2_4764 0.30 +0.3 5,502,282 + Pf6N2E2_4764 0.30 +0.7 5,502,282 + Pf6N2E2_4764 0.30 -1.9 5,502,283 - Pf6N2E2_4764 0.30 -0.4 5,502,283 - Pf6N2E2_4764 0.30 -0.3 5,502,283 - Pf6N2E2_4764 0.30 +0.1 5,502,283 - Pf6N2E2_4764 0.30 +0.4 5,502,283 - Pf6N2E2_4764 0.30 +0.4 5,502,894 + +1.0 5,502,904 + +0.0 5,502,904 + -0.3 5,502,904 + +0.1 5,502,904 + +0.4 5,502,905 - -0.5 5,502,905 - +0.1 5,502,905 - +0.2 5,502,905 - -0.4 5,502,905 - +0.5 5,502,975 + Pf6N2E2_4765 0.33 +0.9 5,502,976 - Pf6N2E2_4765 0.33 +0.9 5,503,116 + Pf6N2E2_4765 0.80 +1.3 5,503,116 + Pf6N2E2_4765 0.80 +1.0 5,503,116 + Pf6N2E2_4765 0.80 +0.9 5,503,117 - Pf6N2E2_4765 0.81 -0.0 5,503,117 - Pf6N2E2_4765 0.81 +0.4 5,503,117 - Pf6N2E2_4765 0.81 -0.1 5,503,149 + +0.1 5,503,150 - +0.8 5,503,161 + -0.1 5,503,161 + -0.1 5,503,162 - -1.5 5,503,236 + +1.1 5,503,236 + +0.1 5,503,237 - -3.0 5,503,237 - -0.7 5,503,237 - -1.9 5,503,237 - +0.5 5,503,237 - +0.3 5,503,286 + -0.5 5,503,286 + -0.9 5,503,286 + -0.1 5,503,368 + +0.7 5,503,368 + +0.2 5,503,368 + +0.0 5,503,368 + +0.3 5,503,369 - +0.0 5,503,369 - -0.6 5,503,394 + -0.1 5,503,395 - -1.6 5,503,427 + +1.0 5,503,428 - -0.3 5,503,428 - -0.6 5,503,444 - +0.0 5,503,449 + -0.2 5,503,460 - -1.4 5,503,460 - -0.4 5,503,460 - -1.3 5,503,460 - -0.5 5,503,460 - +0.0 5,503,460 - -1.0 5,503,522 + +0.6 5,503,523 - -2.3 5,503,696 + Pf6N2E2_4766 0.14 -0.8 5,503,696 + Pf6N2E2_4766 0.14 -0.4 5,503,696 + Pf6N2E2_4766 0.14 -1.2 5,503,696 + Pf6N2E2_4766 0.14 +0.7 5,503,697 - Pf6N2E2_4766 0.14 +0.3 5,503,697 - Pf6N2E2_4766 0.14 +0.0 5,503,697 - Pf6N2E2_4766 0.14 -0.9 5,503,697 - Pf6N2E2_4766 0.14 -0.2 5,503,828 + Pf6N2E2_4766 0.21 +0.1 5,503,829 - Pf6N2E2_4766 0.21 -0.2 5,503,897 + Pf6N2E2_4766 0.25 -1.9 5,503,897 + Pf6N2E2_4766 0.25 -0.5 5,503,897 + Pf6N2E2_4766 0.25 -0.7 5,503,897 + Pf6N2E2_4766 0.25 -0.6 5,503,897 + Pf6N2E2_4766 0.25 -0.5 5,504,014 + Pf6N2E2_4766 0.31 +0.2 5,504,014 + Pf6N2E2_4766 0.31 +0.2 5,504,014 + Pf6N2E2_4766 0.31 -1.0 5,504,014 + Pf6N2E2_4766 0.31 +0.5 5,504,014 + Pf6N2E2_4766 0.31 -1.1 5,504,014 + Pf6N2E2_4766 0.31 +0.9 5,504,014 + Pf6N2E2_4766 0.31 +0.3 5,504,015 - Pf6N2E2_4766 0.31 +0.1 5,504,015 - Pf6N2E2_4766 0.31 -0.5 5,504,015 - Pf6N2E2_4766 0.31 +0.8 5,504,015 - Pf6N2E2_4766 0.31 +0.6 5,504,119 - Pf6N2E2_4766 0.37 -0.5
Or see this region's nucleotide sequence