Experiment: Chloramphenicol 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4437 and Pf6N2E2_4438 overlap by 4 nucleotides Pf6N2E2_4438 and Pf6N2E2_4439 are separated by 50 nucleotides Pf6N2E2_4439 and Pf6N2E2_4440 overlap by 1 nucleotides Pf6N2E2_4440 and Pf6N2E2_4441 are separated by 143 nucleotides
Pf6N2E2_4437 - Twin-arginine translocation protein TatB, at 5,168,942 to 5,170,117
_4437
Pf6N2E2_4438 - hypothetical protein, at 5,170,114 to 5,170,578
_4438
Pf6N2E2_4439 - Glutathione-regulated potassium-efflux system ATP-binding protein, at 5,170,629 to 5,172,539
_4439
Pf6N2E2_4440 - Small-conductance mechanosensitive channel, at 5,172,539 to 5,173,120
_4440
Pf6N2E2_4441 - Homoserine/homoserine lactone efflux protein, at 5,173,264 to 5,173,896
_4441
Position (kb)
5170
5171
5172
5173 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5169.647 kb on - strand, within Pf6N2E2_4437 at 5169.685 kb on - strand, within Pf6N2E2_4437 at 5170.115 kb on + strand at 5170.115 kb on + strand at 5170.116 kb on - strand at 5170.405 kb on + strand, within Pf6N2E2_4438 at 5170.405 kb on + strand, within Pf6N2E2_4438 at 5170.405 kb on + strand, within Pf6N2E2_4438 at 5170.406 kb on - strand, within Pf6N2E2_4438 at 5170.406 kb on - strand, within Pf6N2E2_4438 at 5170.525 kb on + strand, within Pf6N2E2_4438 at 5170.526 kb on - strand, within Pf6N2E2_4438 at 5170.541 kb on + strand at 5170.541 kb on + strand at 5170.541 kb on + strand at 5170.541 kb on + strand at 5170.584 kb on + strand at 5170.612 kb on + strand at 5170.612 kb on + strand at 5170.628 kb on + strand at 5170.629 kb on - strand at 5170.629 kb on - strand at 5170.654 kb on + strand at 5170.654 kb on + strand at 5170.655 kb on - strand at 5170.678 kb on + strand at 5170.678 kb on + strand at 5170.679 kb on - strand at 5170.679 kb on - strand at 5170.875 kb on + strand, within Pf6N2E2_4439 at 5170.875 kb on + strand, within Pf6N2E2_4439 at 5170.875 kb on + strand, within Pf6N2E2_4439 at 5170.875 kb on + strand, within Pf6N2E2_4439 at 5170.876 kb on - strand, within Pf6N2E2_4439 at 5170.876 kb on - strand, within Pf6N2E2_4439 at 5170.995 kb on + strand, within Pf6N2E2_4439 at 5170.996 kb on - strand, within Pf6N2E2_4439 at 5171.042 kb on + strand, within Pf6N2E2_4439 at 5171.307 kb on + strand, within Pf6N2E2_4439 at 5171.307 kb on + strand, within Pf6N2E2_4439 at 5171.308 kb on - strand, within Pf6N2E2_4439 at 5171.321 kb on + strand, within Pf6N2E2_4439 at 5171.373 kb on + strand, within Pf6N2E2_4439 at 5171.412 kb on + strand, within Pf6N2E2_4439 at 5171.412 kb on + strand, within Pf6N2E2_4439 at 5171.412 kb on + strand, within Pf6N2E2_4439 at 5171.412 kb on + strand, within Pf6N2E2_4439 at 5171.412 kb on + strand, within Pf6N2E2_4439 at 5171.413 kb on - strand, within Pf6N2E2_4439 at 5171.413 kb on - strand, within Pf6N2E2_4439 at 5171.678 kb on + strand, within Pf6N2E2_4439 at 5171.678 kb on + strand, within Pf6N2E2_4439 at 5172.171 kb on + strand, within Pf6N2E2_4439 at 5172.171 kb on + strand, within Pf6N2E2_4439 at 5172.172 kb on - strand, within Pf6N2E2_4439 at 5172.394 kb on - strand at 5172.559 kb on + strand at 5172.559 kb on + strand at 5172.559 kb on + strand at 5172.559 kb on + strand at 5172.559 kb on + strand at 5172.559 kb on + strand at 5172.560 kb on - strand at 5172.789 kb on + strand, within Pf6N2E2_4440 at 5172.790 kb on - strand, within Pf6N2E2_4440 at 5172.853 kb on - strand, within Pf6N2E2_4440 at 5172.884 kb on + strand, within Pf6N2E2_4440 at 5172.884 kb on + strand, within Pf6N2E2_4440 at 5172.884 kb on + strand, within Pf6N2E2_4440 at 5172.885 kb on - strand, within Pf6N2E2_4440 at 5172.885 kb on - strand, within Pf6N2E2_4440 at 5172.885 kb on - strand, within Pf6N2E2_4440 at 5172.885 kb on - strand, within Pf6N2E2_4440 at 5172.885 kb on - strand, within Pf6N2E2_4440 at 5172.983 kb on + strand, within Pf6N2E2_4440 at 5172.983 kb on + strand, within Pf6N2E2_4440 at 5172.983 kb on + strand, within Pf6N2E2_4440 at 5172.984 kb on - strand, within Pf6N2E2_4440 at 5172.984 kb on - strand, within Pf6N2E2_4440 at 5172.984 kb on - strand, within Pf6N2E2_4440 at 5173.038 kb on + strand, within Pf6N2E2_4440 at 5173.038 kb on + strand, within Pf6N2E2_4440 at 5173.118 kb on + strand at 5173.118 kb on + strand at 5173.130 kb on - strand at 5173.180 kb on - strand at 5173.224 kb on + strand at 5173.224 kb on + strand at 5173.224 kb on + strand at 5173.224 kb on + strand at 5173.224 kb on + strand at 5173.225 kb on - strand at 5173.225 kb on - strand at 5173.225 kb on - strand at 5173.225 kb on - strand at 5173.225 kb on - strand at 5173.225 kb on - strand at 5173.229 kb on + strand at 5173.230 kb on - strand at 5173.480 kb on + strand, within Pf6N2E2_4441 at 5173.480 kb on + strand, within Pf6N2E2_4441 at 5173.481 kb on - strand, within Pf6N2E2_4441 at 5173.481 kb on - strand, within Pf6N2E2_4441 at 5173.481 kb on - strand, within Pf6N2E2_4441 at 5173.481 kb on - strand, within Pf6N2E2_4441 at 5173.481 kb on - strand, within Pf6N2E2_4441 at 5173.507 kb on + strand, within Pf6N2E2_4441 at 5173.507 kb on + strand, within Pf6N2E2_4441 at 5173.516 kb on + strand, within Pf6N2E2_4441
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.008 mg/ml remove 5,169,647 - Pf6N2E2_4437 0.60 -1.1 5,169,685 - Pf6N2E2_4437 0.63 +0.9 5,170,115 + -0.2 5,170,115 + +0.6 5,170,116 - +0.1 5,170,405 + Pf6N2E2_4438 0.63 -1.9 5,170,405 + Pf6N2E2_4438 0.63 +0.9 5,170,405 + Pf6N2E2_4438 0.63 -1.3 5,170,406 - Pf6N2E2_4438 0.63 -0.3 5,170,406 - Pf6N2E2_4438 0.63 -0.7 5,170,525 + Pf6N2E2_4438 0.88 -0.1 5,170,526 - Pf6N2E2_4438 0.89 +0.8 5,170,541 + +0.5 5,170,541 + -1.0 5,170,541 + +0.2 5,170,541 + +0.4 5,170,584 + +1.3 5,170,612 + -1.7 5,170,612 + +0.5 5,170,628 + -0.3 5,170,629 - -1.5 5,170,629 - -2.0 5,170,654 + -1.3 5,170,654 + -0.8 5,170,655 - -2.4 5,170,678 + -0.7 5,170,678 + -0.7 5,170,679 - -1.5 5,170,679 - -1.8 5,170,875 + Pf6N2E2_4439 0.13 -1.7 5,170,875 + Pf6N2E2_4439 0.13 -0.7 5,170,875 + Pf6N2E2_4439 0.13 -0.7 5,170,875 + Pf6N2E2_4439 0.13 -1.4 5,170,876 - Pf6N2E2_4439 0.13 -2.1 5,170,876 - Pf6N2E2_4439 0.13 -0.3 5,170,995 + Pf6N2E2_4439 0.19 -2.2 5,170,996 - Pf6N2E2_4439 0.19 -1.6 5,171,042 + Pf6N2E2_4439 0.22 -2.6 5,171,307 + Pf6N2E2_4439 0.35 -2.3 5,171,307 + Pf6N2E2_4439 0.35 -0.9 5,171,308 - Pf6N2E2_4439 0.36 -0.3 5,171,321 + Pf6N2E2_4439 0.36 -1.3 5,171,373 + Pf6N2E2_4439 0.39 -0.5 5,171,412 + Pf6N2E2_4439 0.41 -2.9 5,171,412 + Pf6N2E2_4439 0.41 -1.3 5,171,412 + Pf6N2E2_4439 0.41 -0.1 5,171,412 + Pf6N2E2_4439 0.41 -2.2 5,171,412 + Pf6N2E2_4439 0.41 -3.5 5,171,413 - Pf6N2E2_4439 0.41 -1.3 5,171,413 - Pf6N2E2_4439 0.41 -1.0 5,171,678 + Pf6N2E2_4439 0.55 -2.2 5,171,678 + Pf6N2E2_4439 0.55 -0.8 5,172,171 + Pf6N2E2_4439 0.81 -1.0 5,172,171 + Pf6N2E2_4439 0.81 -2.0 5,172,172 - Pf6N2E2_4439 0.81 -3.4 5,172,394 - -2.8 5,172,559 + +0.2 5,172,559 + +0.9 5,172,559 + -0.4 5,172,559 + +1.4 5,172,559 + +1.2 5,172,559 + +0.1 5,172,560 - -0.0 5,172,789 + Pf6N2E2_4440 0.43 +0.0 5,172,790 - Pf6N2E2_4440 0.43 -0.8 5,172,853 - Pf6N2E2_4440 0.54 -0.2 5,172,884 + Pf6N2E2_4440 0.59 +2.4 5,172,884 + Pf6N2E2_4440 0.59 +0.4 5,172,884 + Pf6N2E2_4440 0.59 +0.2 5,172,885 - Pf6N2E2_4440 0.59 +0.2 5,172,885 - Pf6N2E2_4440 0.59 -0.2 5,172,885 - Pf6N2E2_4440 0.59 -0.6 5,172,885 - Pf6N2E2_4440 0.59 -1.0 5,172,885 - Pf6N2E2_4440 0.59 +1.1 5,172,983 + Pf6N2E2_4440 0.76 -0.8 5,172,983 + Pf6N2E2_4440 0.76 -0.1 5,172,983 + Pf6N2E2_4440 0.76 +0.2 5,172,984 - Pf6N2E2_4440 0.76 -0.9 5,172,984 - Pf6N2E2_4440 0.76 -0.5 5,172,984 - Pf6N2E2_4440 0.76 +1.4 5,173,038 + Pf6N2E2_4440 0.86 +0.5 5,173,038 + Pf6N2E2_4440 0.86 +0.4 5,173,118 + -0.7 5,173,118 + +1.0 5,173,130 - -0.3 5,173,180 - -0.5 5,173,224 + +0.3 5,173,224 + -0.1 5,173,224 + +0.7 5,173,224 + +0.4 5,173,224 + -0.3 5,173,225 - -0.5 5,173,225 - -0.1 5,173,225 - -0.1 5,173,225 - +0.2 5,173,225 - -0.0 5,173,225 - -0.7 5,173,229 + -0.2 5,173,230 - -1.5 5,173,480 + Pf6N2E2_4441 0.34 -0.4 5,173,480 + Pf6N2E2_4441 0.34 -0.3 5,173,481 - Pf6N2E2_4441 0.34 +0.6 5,173,481 - Pf6N2E2_4441 0.34 +0.1 5,173,481 - Pf6N2E2_4441 0.34 +0.9 5,173,481 - Pf6N2E2_4441 0.34 +0.4 5,173,481 - Pf6N2E2_4441 0.34 +1.0 5,173,507 + Pf6N2E2_4441 0.38 -0.5 5,173,507 + Pf6N2E2_4441 0.38 -0.0 5,173,516 + Pf6N2E2_4441 0.40 -0.7
Or see this region's nucleotide sequence