Experiment: Chloramphenicol 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1816 and Pf6N2E2_1817 overlap by 4 nucleotides Pf6N2E2_1817 and Pf6N2E2_1818 are separated by 46 nucleotides Pf6N2E2_1818 and Pf6N2E2_1819 are separated by 137 nucleotides
Pf6N2E2_1816 - Pyruvate dehydrogenase E1 component (EC 1.2.4.1), at 2,148,793 to 2,151,489
_1816
Pf6N2E2_1817 - hypothetical protein, at 2,151,486 to 2,151,908
_1817
Pf6N2E2_1818 - hypothetical protein, at 2,151,955 to 2,152,413
_1818
Pf6N2E2_1819 - 1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7), at 2,152,551 to 2,153,567
_1819
Position (kb)
2151
2152 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2150.850 kb on - strand, within Pf6N2E2_1816 at 2150.850 kb on - strand, within Pf6N2E2_1816 at 2151.030 kb on - strand, within Pf6N2E2_1816 at 2151.030 kb on - strand, within Pf6N2E2_1816 at 2151.056 kb on + strand, within Pf6N2E2_1816 at 2151.057 kb on - strand, within Pf6N2E2_1816 at 2151.057 kb on - strand, within Pf6N2E2_1816 at 2151.143 kb on + strand at 2151.144 kb on - strand, within Pf6N2E2_1816 at 2151.144 kb on - strand, within Pf6N2E2_1816 at 2151.288 kb on + strand at 2151.297 kb on - strand at 2151.297 kb on - strand at 2151.489 kb on + strand at 2151.490 kb on - strand at 2151.559 kb on - strand, within Pf6N2E2_1817 at 2151.559 kb on - strand, within Pf6N2E2_1817 at 2151.559 kb on - strand, within Pf6N2E2_1817 at 2151.568 kb on - strand, within Pf6N2E2_1817 at 2151.845 kb on + strand, within Pf6N2E2_1817 at 2151.909 kb on - strand at 2152.181 kb on + strand, within Pf6N2E2_1818 at 2152.182 kb on - strand, within Pf6N2E2_1818 at 2152.271 kb on + strand, within Pf6N2E2_1818 at 2152.294 kb on + strand, within Pf6N2E2_1818 at 2152.295 kb on - strand, within Pf6N2E2_1818 at 2152.322 kb on + strand, within Pf6N2E2_1818 at 2152.322 kb on + strand, within Pf6N2E2_1818 at 2152.322 kb on + strand, within Pf6N2E2_1818 at 2152.323 kb on - strand, within Pf6N2E2_1818 at 2152.577 kb on - strand at 2152.687 kb on + strand, within Pf6N2E2_1819 at 2152.845 kb on - strand, within Pf6N2E2_1819
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.008 mg/ml remove 2,150,850 - Pf6N2E2_1816 0.76 -2.3 2,150,850 - Pf6N2E2_1816 0.76 -0.0 2,151,030 - Pf6N2E2_1816 0.83 -0.8 2,151,030 - Pf6N2E2_1816 0.83 -0.1 2,151,056 + Pf6N2E2_1816 0.84 +0.6 2,151,057 - Pf6N2E2_1816 0.84 +0.3 2,151,057 - Pf6N2E2_1816 0.84 -0.5 2,151,143 + +2.0 2,151,144 - Pf6N2E2_1816 0.87 -0.2 2,151,144 - Pf6N2E2_1816 0.87 -0.1 2,151,288 + +0.8 2,151,297 - +0.6 2,151,297 - -0.6 2,151,489 + +0.9 2,151,490 - -1.1 2,151,559 - Pf6N2E2_1817 0.17 -0.7 2,151,559 - Pf6N2E2_1817 0.17 -1.3 2,151,559 - Pf6N2E2_1817 0.17 +0.3 2,151,568 - Pf6N2E2_1817 0.19 -0.7 2,151,845 + Pf6N2E2_1817 0.85 -0.6 2,151,909 - -0.2 2,152,181 + Pf6N2E2_1818 0.49 +0.2 2,152,182 - Pf6N2E2_1818 0.49 -0.2 2,152,271 + Pf6N2E2_1818 0.69 -0.5 2,152,294 + Pf6N2E2_1818 0.74 +0.8 2,152,295 - Pf6N2E2_1818 0.74 -0.2 2,152,322 + Pf6N2E2_1818 0.80 +0.0 2,152,322 + Pf6N2E2_1818 0.80 +0.5 2,152,322 + Pf6N2E2_1818 0.80 +0.8 2,152,323 - Pf6N2E2_1818 0.80 +0.5 2,152,577 - +1.4 2,152,687 + Pf6N2E2_1819 0.13 -0.7 2,152,845 - Pf6N2E2_1819 0.29 +0.7
Or see this region's nucleotide sequence