Experiment: Chloramphenicol 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_178 and Pf6N2E2_179 are separated by 3 nucleotides Pf6N2E2_179 and Pf6N2E2_180 overlap by 20 nucleotides Pf6N2E2_180 and Pf6N2E2_6104 are separated by 90 nucleotides Pf6N2E2_6104 and Pf6N2E2_181 are separated by 193 nucleotides
Pf6N2E2_178 - Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20), at 227,923 to 230,301
_178
Pf6N2E2_179 - Integration host factor alpha subunit, at 230,305 to 230,607
_179
Pf6N2E2_180 - Transcriptional regulator, MerR family, at 230,588 to 230,944
_180
Pf6N2E2_6104 - tRNA-Pro-GGG, at 231,035 to 231,108
_6104
Pf6N2E2_181 - Two-component response regulator, at 231,302 to 231,994
_181
Position (kb) 230
231 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 230.304 kb on + strand at 230.304 kb on + strand at 230.304 kb on + strand at 230.305 kb on - strand at 230.587 kb on + strand at 230.587 kb on + strand at 230.587 kb on + strand at 230.587 kb on + strand at 230.587 kb on + strand at 230.588 kb on - strand at 230.588 kb on - strand at 230.605 kb on + strand at 230.756 kb on + strand, within Pf6N2E2_180 at 230.756 kb on + strand, within Pf6N2E2_180 at 230.756 kb on + strand, within Pf6N2E2_180 at 230.804 kb on + strand, within Pf6N2E2_180 at 230.869 kb on + strand, within Pf6N2E2_180 at 230.870 kb on - strand, within Pf6N2E2_180 at 231.128 kb on - strand at 231.218 kb on - strand at 231.283 kb on + strand at 231.283 kb on + strand at 231.283 kb on + strand at 231.283 kb on + strand at 231.283 kb on + strand at 231.283 kb on + strand at 231.284 kb on - strand at 231.374 kb on + strand, within Pf6N2E2_181 at 231.374 kb on + strand, within Pf6N2E2_181 at 231.375 kb on - strand, within Pf6N2E2_181 at 231.375 kb on - strand, within Pf6N2E2_181 at 231.375 kb on - strand, within Pf6N2E2_181 at 231.380 kb on + strand, within Pf6N2E2_181 at 231.380 kb on + strand, within Pf6N2E2_181 at 231.380 kb on + strand, within Pf6N2E2_181 at 231.381 kb on - strand, within Pf6N2E2_181 at 231.381 kb on - strand, within Pf6N2E2_181 at 231.390 kb on + strand, within Pf6N2E2_181 at 231.390 kb on + strand, within Pf6N2E2_181 at 231.390 kb on + strand, within Pf6N2E2_181 at 231.390 kb on + strand, within Pf6N2E2_181 at 231.390 kb on + strand, within Pf6N2E2_181 at 231.390 kb on + strand, within Pf6N2E2_181 at 231.390 kb on + strand, within Pf6N2E2_181 at 231.390 kb on + strand, within Pf6N2E2_181 at 231.390 kb on + strand, within Pf6N2E2_181 at 231.390 kb on + strand, within Pf6N2E2_181 at 231.391 kb on - strand, within Pf6N2E2_181 at 231.391 kb on - strand, within Pf6N2E2_181 at 231.391 kb on - strand, within Pf6N2E2_181 at 231.391 kb on - strand, within Pf6N2E2_181 at 231.391 kb on - strand, within Pf6N2E2_181 at 231.391 kb on - strand, within Pf6N2E2_181 at 231.391 kb on - strand, within Pf6N2E2_181 at 231.391 kb on - strand, within Pf6N2E2_181 at 231.391 kb on - strand, within Pf6N2E2_181 at 231.391 kb on - strand, within Pf6N2E2_181 at 231.667 kb on - strand, within Pf6N2E2_181 at 231.667 kb on - strand, within Pf6N2E2_181 at 231.891 kb on + strand, within Pf6N2E2_181 at 231.892 kb on - strand, within Pf6N2E2_181 at 231.892 kb on - strand, within Pf6N2E2_181
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.008 mg/ml remove 230,304 + -1.2 230,304 + -0.9 230,304 + -2.8 230,305 - -1.2 230,587 + -2.7 230,587 + -1.3 230,587 + -2.5 230,587 + -1.2 230,587 + +0.1 230,588 - +0.0 230,588 - -1.1 230,605 + -0.8 230,756 + Pf6N2E2_180 0.47 -1.0 230,756 + Pf6N2E2_180 0.47 -0.6 230,756 + Pf6N2E2_180 0.47 -0.6 230,804 + Pf6N2E2_180 0.61 -0.4 230,869 + Pf6N2E2_180 0.79 -0.2 230,870 - Pf6N2E2_180 0.79 -0.7 231,128 - +0.2 231,218 - -0.6 231,283 + -0.1 231,283 + -0.7 231,283 + +0.3 231,283 + -0.4 231,283 + -0.7 231,283 + +1.1 231,284 - +0.7 231,374 + Pf6N2E2_181 0.10 +0.8 231,374 + Pf6N2E2_181 0.10 +0.4 231,375 - Pf6N2E2_181 0.11 -0.2 231,375 - Pf6N2E2_181 0.11 -0.6 231,375 - Pf6N2E2_181 0.11 -0.5 231,380 + Pf6N2E2_181 0.11 +0.1 231,380 + Pf6N2E2_181 0.11 +0.7 231,380 + Pf6N2E2_181 0.11 -0.3 231,381 - Pf6N2E2_181 0.11 -0.2 231,381 - Pf6N2E2_181 0.11 -0.0 231,390 + Pf6N2E2_181 0.13 +0.6 231,390 + Pf6N2E2_181 0.13 +0.9 231,390 + Pf6N2E2_181 0.13 +0.0 231,390 + Pf6N2E2_181 0.13 -0.1 231,390 + Pf6N2E2_181 0.13 +0.6 231,390 + Pf6N2E2_181 0.13 -0.7 231,390 + Pf6N2E2_181 0.13 -0.1 231,390 + Pf6N2E2_181 0.13 +0.8 231,390 + Pf6N2E2_181 0.13 -0.0 231,390 + Pf6N2E2_181 0.13 -1.6 231,391 - Pf6N2E2_181 0.13 +0.5 231,391 - Pf6N2E2_181 0.13 +0.8 231,391 - Pf6N2E2_181 0.13 -1.6 231,391 - Pf6N2E2_181 0.13 +0.4 231,391 - Pf6N2E2_181 0.13 -0.2 231,391 - Pf6N2E2_181 0.13 -0.4 231,391 - Pf6N2E2_181 0.13 +0.0 231,391 - Pf6N2E2_181 0.13 -0.6 231,391 - Pf6N2E2_181 0.13 -1.6 231,391 - Pf6N2E2_181 0.13 +1.1 231,667 - Pf6N2E2_181 0.53 -0.4 231,667 - Pf6N2E2_181 0.53 +0.6 231,891 + Pf6N2E2_181 0.85 +0.3 231,892 - Pf6N2E2_181 0.85 +0.6 231,892 - Pf6N2E2_181 0.85 -0.7
Or see this region's nucleotide sequence