Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28020 and BPHYT_RS28025 overlap by 4 nucleotides BPHYT_RS28025 and BPHYT_RS28030 are separated by 24 nucleotides BPHYT_RS28030 and BPHYT_RS28035 are separated by 72 nucleotides
BPHYT_RS28020: BPHYT_RS28020 - enoyl-CoA hydratase, at 1,849,636 to 1,850,454
_RS28020
BPHYT_RS28025: BPHYT_RS28025 - 3-hydroxyisobutyrate dehydrogenase, at 1,850,451 to 1,851,365
_RS28025
BPHYT_RS28030: BPHYT_RS28030 - methylmalonate-semialdehyde dehydrogenase, at 1,851,390 to 1,852,901
_RS28030
BPHYT_RS28035: BPHYT_RS28035 - AMP-dependent synthetase, at 1,852,974 to 1,854,665
_RS28035
Position (kb) 1851
1852
1853 Strain fitness (log2 ratio) -2
-1
0
1 at 1850.438 kb on + strand at 1850.713 kb on + strand, within BPHYT_RS28025 at 1850.938 kb on + strand, within BPHYT_RS28025 at 1851.282 kb on + strand at 1851.500 kb on - strand at 1852.483 kb on + strand, within BPHYT_RS28030 at 1852.483 kb on + strand, within BPHYT_RS28030 at 1852.484 kb on - strand, within BPHYT_RS28030 at 1852.484 kb on - strand, within BPHYT_RS28030 at 1853.098 kb on - strand at 1853.660 kb on + strand, within BPHYT_RS28035
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 1,850,438 + -0.4 1,850,713 + BPHYT_RS28025 0.29 -0.7 1,850,938 + BPHYT_RS28025 0.53 -1.4 1,851,282 + -0.2 1,851,500 - -0.1 1,852,483 + BPHYT_RS28030 0.72 -1.0 1,852,483 + BPHYT_RS28030 0.72 -0.8 1,852,484 - BPHYT_RS28030 0.72 -1.3 1,852,484 - BPHYT_RS28030 0.72 -0.8 1,853,098 - -0.0 1,853,660 + BPHYT_RS28035 0.41 -2.2
Or see this region's nucleotide sequence