Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS06390 and BPHYT_RS06395 are separated by 5 nucleotides BPHYT_RS06395 and BPHYT_RS06400 are separated by 89 nucleotides BPHYT_RS06400 and BPHYT_RS06405 are separated by 327 nucleotides
BPHYT_RS06390: BPHYT_RS06390 - ribosome-associated protein IOJAP, at 1,455,004 to 1,455,468
_RS06390
BPHYT_RS06395: BPHYT_RS06395 - nicotinic acid mononucleotide adenylyltransferase, at 1,455,474 to 1,456,145
_RS06395
BPHYT_RS06400: BPHYT_RS06400 - oxygen-dependent coproporphyrinogen-III oxidase, at 1,456,235 to 1,457,158
_RS06400
BPHYT_RS06405: BPHYT_RS06405 - phosphoribosylamine--glycine ligase, at 1,457,486 to 1,458,784
_RS06405
Position (kb)
1456
1457
1458 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1456.630 kb on - strand, within BPHYT_RS06400 at 1456.674 kb on + strand, within BPHYT_RS06400 at 1456.819 kb on + strand, within BPHYT_RS06400 at 1456.820 kb on - strand, within BPHYT_RS06400 at 1456.820 kb on - strand, within BPHYT_RS06400 at 1456.820 kb on - strand, within BPHYT_RS06400 at 1457.005 kb on - strand, within BPHYT_RS06400 at 1457.260 kb on + strand at 1457.261 kb on - strand at 1457.397 kb on + strand at 1457.398 kb on - strand at 1457.398 kb on - strand at 1457.770 kb on - strand, within BPHYT_RS06405 at 1457.775 kb on + strand, within BPHYT_RS06405 at 1457.775 kb on + strand, within BPHYT_RS06405 at 1457.872 kb on - strand, within BPHYT_RS06405 at 1457.929 kb on + strand, within BPHYT_RS06405 at 1457.930 kb on - strand, within BPHYT_RS06405 at 1458.024 kb on - strand, within BPHYT_RS06405 at 1458.029 kb on - strand, within BPHYT_RS06405
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 1,456,630 - BPHYT_RS06400 0.43 -1.2 1,456,674 + BPHYT_RS06400 0.48 -0.6 1,456,819 + BPHYT_RS06400 0.63 -1.5 1,456,820 - BPHYT_RS06400 0.63 -1.2 1,456,820 - BPHYT_RS06400 0.63 -3.2 1,456,820 - BPHYT_RS06400 0.63 -1.1 1,457,005 - BPHYT_RS06400 0.83 +0.3 1,457,260 + -0.2 1,457,261 - +0.1 1,457,397 + -1.3 1,457,398 - +0.1 1,457,398 - +1.3 1,457,770 - BPHYT_RS06405 0.22 -3.2 1,457,775 + BPHYT_RS06405 0.22 -6.0 1,457,775 + BPHYT_RS06405 0.22 -5.2 1,457,872 - BPHYT_RS06405 0.30 -3.5 1,457,929 + BPHYT_RS06405 0.34 -4.2 1,457,930 - BPHYT_RS06405 0.34 -4.0 1,458,024 - BPHYT_RS06405 0.41 -4.2 1,458,029 - BPHYT_RS06405 0.42 -3.4
Or see this region's nucleotide sequence