Experiment: Tetracycline 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_4461 and Ac3H11_4462 are separated by 22 nucleotides Ac3H11_4462 and Ac3H11_4463 overlap by 4 nucleotides Ac3H11_4463 and Ac3H11_4464 are separated by 45 nucleotides Ac3H11_4464 and Ac3H11_4693 are separated by 52 nucleotides
Ac3H11_4461 - Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-), at 43,412 to 44,710
_4461
Ac3H11_4462 - Probable proline rich signal peptide protein, at 44,733 to 45,344
_4462
Ac3H11_4463 - Nitrogen regulation protein NtrY (EC 2.7.3.-), at 45,341 to 47,632
_4463
Ac3H11_4464 - Probable two component response regulator transcription regulator protein, at 47,678 to 48,361
_4464
Ac3H11_4693 - tRNA-Phe-GAA, at 48,414 to 48,486
_4693
Position (kb) 45
46
47
48 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 44.513 kb on + strand, within Ac3H11_4461 at 44.513 kb on + strand, within Ac3H11_4461 at 44.513 kb on + strand, within Ac3H11_4461 at 44.513 kb on + strand, within Ac3H11_4461 at 44.513 kb on + strand, within Ac3H11_4461 at 44.513 kb on + strand, within Ac3H11_4461 at 44.513 kb on + strand, within Ac3H11_4461 at 44.514 kb on - strand, within Ac3H11_4461 at 44.514 kb on - strand, within Ac3H11_4461 at 44.514 kb on - strand, within Ac3H11_4461 at 44.514 kb on - strand, within Ac3H11_4461 at 44.514 kb on - strand, within Ac3H11_4461 at 44.514 kb on - strand, within Ac3H11_4461 at 44.613 kb on + strand at 44.613 kb on + strand at 44.613 kb on + strand at 44.708 kb on + strand at 44.708 kb on + strand at 45.518 kb on + strand at 45.518 kb on + strand at 47.616 kb on + strand at 47.633 kb on + strand at 47.633 kb on + strand at 47.633 kb on + strand at 48.359 kb on + strand at 48.359 kb on + strand at 48.359 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0001 mg/ml remove 44,513 + Ac3H11_4461 0.85 -3.2 44,513 + Ac3H11_4461 0.85 -2.4 44,513 + Ac3H11_4461 0.85 -3.6 44,513 + Ac3H11_4461 0.85 +0.9 44,513 + Ac3H11_4461 0.85 -3.7 44,513 + Ac3H11_4461 0.85 -0.2 44,513 + Ac3H11_4461 0.85 -3.9 44,514 - Ac3H11_4461 0.85 -3.1 44,514 - Ac3H11_4461 0.85 -2.6 44,514 - Ac3H11_4461 0.85 -3.3 44,514 - Ac3H11_4461 0.85 -2.7 44,514 - Ac3H11_4461 0.85 -1.1 44,514 - Ac3H11_4461 0.85 -1.9 44,613 + -3.6 44,613 + -2.5 44,613 + -1.3 44,708 + +0.2 44,708 + +1.7 45,518 + -1.1 45,518 + +0.9 47,616 + -0.6 47,633 + -2.2 47,633 + -3.0 47,633 + -4.5 48,359 + -0.6 48,359 + -0.0 48,359 + -0.1
Or see this region's nucleotide sequence