Experiment: Tetracycline 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3450 and Ac3H11_3451 are separated by 162 nucleotides Ac3H11_3451 and Ac3H11_3452 are separated by 161 nucleotides Ac3H11_3452 and Ac3H11_3648 are separated by 50 nucleotides Ac3H11_3648 and Ac3H11_3453 are separated by 30 nucleotides
Ac3H11_3450 - Peptidyl-tRNA hydrolase (EC 3.1.1.29), at 243 to 851
_3450
Ac3H11_3451 - LSU ribosomal protein L25p, at 1,014 to 1,649
_3451
Ac3H11_3452 - Ribose-phosphate pyrophosphokinase (EC 2.7.6.1), at 1,811 to 2,785
_3452
Ac3H11_3648 - tRNA-Gln-TTG, at 2,836 to 2,909
_3648
Ac3H11_3453 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (EC 2.7.1.148), at 2,940 to 3,779
_3453
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-1
0
1 at 1.015 kb on + strand at 1.015 kb on + strand at 1.015 kb on + strand at 1.015 kb on + strand at 1.016 kb on - strand at 1.016 kb on - strand at 1.016 kb on - strand at 1.016 kb on - strand at 1.330 kb on + strand, within Ac3H11_3451 at 1.330 kb on + strand, within Ac3H11_3451 at 1.330 kb on + strand, within Ac3H11_3451 at 1.330 kb on + strand, within Ac3H11_3451 at 1.331 kb on - strand, within Ac3H11_3451 at 2.830 kb on - strand at 2.830 kb on - strand at 2.830 kb on - strand at 2.830 kb on - strand at 2.830 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0001 mg/ml remove 1,015 + -1.1 1,015 + -1.0 1,015 + +0.2 1,015 + -1.4 1,016 - +0.3 1,016 - -0.5 1,016 - +1.3 1,016 - +0.0 1,330 + Ac3H11_3451 0.50 -0.7 1,330 + Ac3H11_3451 0.50 -1.3 1,330 + Ac3H11_3451 0.50 -0.4 1,330 + Ac3H11_3451 0.50 -1.4 1,331 - Ac3H11_3451 0.50 -0.7 2,830 - -0.1 2,830 - -0.7 2,830 - -0.7 2,830 - -0.2 2,830 - +0.1
Or see this region's nucleotide sequence