Experiment: Tetracycline 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_151 and Ac3H11_152 are separated by 36 nucleotides Ac3H11_152 and Ac3H11_153 are separated by 106 nucleotides Ac3H11_153 and Ac3H11_154 are separated by 106 nucleotides
Ac3H11_151 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 35,127 to 36,611
_151
Ac3H11_152 - hypothetical protein, at 36,648 to 36,797
_152
Ac3H11_153 - Similar to 5-oxoprolinase (EC 3.5.2.9) and Methylhydantoinases A, B (EC 3.5.2.14), contradiction in experimental data (see Annotation), at 36,904 to 40,548
_153
Ac3H11_154 - TRAP transporter solute receptor, unknown substrate 5, at 40,655 to 41,620
_154
Position (kb)
36
37
38
39
40
41 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 36.205 kb on + strand, within Ac3H11_151 at 36.444 kb on + strand, within Ac3H11_151 at 36.444 kb on + strand, within Ac3H11_151 at 36.650 kb on - strand at 36.780 kb on - strand, within Ac3H11_152 at 36.791 kb on - strand at 37.199 kb on + strand at 37.368 kb on - strand, within Ac3H11_153 at 37.604 kb on + strand, within Ac3H11_153 at 37.604 kb on + strand, within Ac3H11_153 at 37.605 kb on - strand, within Ac3H11_153 at 37.605 kb on - strand, within Ac3H11_153 at 37.605 kb on - strand, within Ac3H11_153 at 37.605 kb on - strand, within Ac3H11_153 at 37.829 kb on + strand, within Ac3H11_153 at 37.830 kb on - strand, within Ac3H11_153 at 37.830 kb on - strand, within Ac3H11_153 at 37.830 kb on - strand, within Ac3H11_153 at 37.830 kb on - strand, within Ac3H11_153 at 37.830 kb on - strand, within Ac3H11_153 at 38.136 kb on - strand, within Ac3H11_153 at 38.136 kb on - strand, within Ac3H11_153 at 38.165 kb on + strand, within Ac3H11_153 at 38.231 kb on + strand, within Ac3H11_153 at 38.232 kb on - strand, within Ac3H11_153 at 38.232 kb on - strand, within Ac3H11_153 at 38.232 kb on - strand, within Ac3H11_153 at 38.232 kb on - strand, within Ac3H11_153 at 38.400 kb on - strand, within Ac3H11_153 at 38.501 kb on + strand, within Ac3H11_153 at 38.691 kb on - strand, within Ac3H11_153 at 38.919 kb on - strand, within Ac3H11_153 at 38.919 kb on - strand, within Ac3H11_153 at 39.105 kb on - strand, within Ac3H11_153 at 39.414 kb on - strand, within Ac3H11_153 at 39.414 kb on - strand, within Ac3H11_153 at 39.480 kb on - strand, within Ac3H11_153 at 39.480 kb on - strand, within Ac3H11_153 at 39.542 kb on + strand, within Ac3H11_153 at 39.894 kb on - strand, within Ac3H11_153 at 39.894 kb on - strand, within Ac3H11_153 at 39.894 kb on - strand, within Ac3H11_153 at 40.173 kb on - strand, within Ac3H11_153 at 40.192 kb on + strand at 40.193 kb on - strand at 40.193 kb on - strand at 40.193 kb on - strand at 40.193 kb on - strand at 40.193 kb on - strand at 40.573 kb on - strand at 40.573 kb on - strand at 40.656 kb on + strand at 40.656 kb on + strand at 40.656 kb on + strand at 40.668 kb on + strand at 40.669 kb on - strand at 40.968 kb on + strand, within Ac3H11_154 at 40.969 kb on - strand, within Ac3H11_154 at 40.969 kb on - strand, within Ac3H11_154 at 41.133 kb on + strand, within Ac3H11_154 at 41.133 kb on + strand, within Ac3H11_154 at 41.134 kb on - strand, within Ac3H11_154 at 41.220 kb on + strand, within Ac3H11_154 at 41.220 kb on + strand, within Ac3H11_154 at 41.221 kb on - strand, within Ac3H11_154
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0001 mg/ml remove 36,205 + Ac3H11_151 0.73 +0.8 36,444 + Ac3H11_151 0.89 -0.9 36,444 + Ac3H11_151 0.89 +0.0 36,650 - +0.1 36,780 - Ac3H11_152 0.88 -1.4 36,791 - -0.9 37,199 + -1.0 37,368 - Ac3H11_153 0.13 +0.3 37,604 + Ac3H11_153 0.19 -0.4 37,604 + Ac3H11_153 0.19 +0.4 37,605 - Ac3H11_153 0.19 +0.5 37,605 - Ac3H11_153 0.19 +1.9 37,605 - Ac3H11_153 0.19 -0.3 37,605 - Ac3H11_153 0.19 +0.0 37,829 + Ac3H11_153 0.25 -0.6 37,830 - Ac3H11_153 0.25 -0.4 37,830 - Ac3H11_153 0.25 +2.2 37,830 - Ac3H11_153 0.25 -0.5 37,830 - Ac3H11_153 0.25 -0.3 37,830 - Ac3H11_153 0.25 -0.0 38,136 - Ac3H11_153 0.34 -0.1 38,136 - Ac3H11_153 0.34 +0.0 38,165 + Ac3H11_153 0.35 -0.0 38,231 + Ac3H11_153 0.36 -0.1 38,232 - Ac3H11_153 0.36 +0.0 38,232 - Ac3H11_153 0.36 -0.5 38,232 - Ac3H11_153 0.36 +0.4 38,232 - Ac3H11_153 0.36 +1.2 38,400 - Ac3H11_153 0.41 -1.9 38,501 + Ac3H11_153 0.44 -1.0 38,691 - Ac3H11_153 0.49 +0.8 38,919 - Ac3H11_153 0.55 +0.5 38,919 - Ac3H11_153 0.55 +0.5 39,105 - Ac3H11_153 0.60 -0.2 39,414 - Ac3H11_153 0.69 -1.5 39,414 - Ac3H11_153 0.69 +1.1 39,480 - Ac3H11_153 0.71 -0.0 39,480 - Ac3H11_153 0.71 -1.2 39,542 + Ac3H11_153 0.72 -1.0 39,894 - Ac3H11_153 0.82 +0.4 39,894 - Ac3H11_153 0.82 -0.4 39,894 - Ac3H11_153 0.82 -0.2 40,173 - Ac3H11_153 0.90 -0.6 40,192 + -0.0 40,193 - -0.2 40,193 - -0.2 40,193 - +0.3 40,193 - +1.1 40,193 - -0.9 40,573 - -0.4 40,573 - -0.2 40,656 + +0.3 40,656 + -0.6 40,656 + -0.9 40,668 + -0.2 40,669 - +0.3 40,968 + Ac3H11_154 0.32 -0.4 40,969 - Ac3H11_154 0.33 -1.0 40,969 - Ac3H11_154 0.33 +1.5 41,133 + Ac3H11_154 0.49 +0.9 41,133 + Ac3H11_154 0.49 -0.1 41,134 - Ac3H11_154 0.50 -0.1 41,220 + Ac3H11_154 0.58 +0.2 41,220 + Ac3H11_154 0.58 +0.5 41,221 - Ac3H11_154 0.59 -0.6
Or see this region's nucleotide sequence