Experiment: Fusidic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS16850 and ABZR86_RS16855 are separated by 97 nucleotides ABZR86_RS16855 and ABZR86_RS16860 overlap by 4 nucleotides ABZR86_RS16860 and ABZR86_RS16865 are separated by 109 nucleotides ABZR86_RS16865 and ABZR86_RS16870 overlap by 1 nucleotides
ABZR86_RS16850: ABZR86_RS16850 - DsbA family oxidoreductase, at 3,804,458 to 3,805,105
_RS16850
ABZR86_RS16855: ABZR86_RS16855 - DUF1993 family protein, at 3,805,203 to 3,805,709
_RS16855
ABZR86_RS16860: ABZR86_RS16860 - TCR/Tet family MFS transporter, at 3,805,706 to 3,807,070
_RS16860
ABZR86_RS16865: ABZR86_RS16865 - EF-hand domain-containing protein, at 3,807,180 to 3,807,515
_RS16865
ABZR86_RS16870: ABZR86_RS16870 - GTP cyclohydrolase I FolE, at 3,807,515 to 3,808,114
_RS16870
Position (kb)
3805
3806
3807
3808 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3804.720 kb on + strand, within ABZR86_RS16850 at 3804.721 kb on - strand, within ABZR86_RS16850 at 3804.807 kb on + strand, within ABZR86_RS16850 at 3804.807 kb on + strand, within ABZR86_RS16850 at 3804.807 kb on + strand, within ABZR86_RS16850 at 3804.807 kb on + strand, within ABZR86_RS16850 at 3804.807 kb on + strand, within ABZR86_RS16850 at 3804.807 kb on + strand, within ABZR86_RS16850 at 3804.808 kb on - strand, within ABZR86_RS16850 at 3804.808 kb on - strand, within ABZR86_RS16850 at 3804.909 kb on + strand, within ABZR86_RS16850 at 3804.909 kb on + strand, within ABZR86_RS16850 at 3804.910 kb on - strand, within ABZR86_RS16850 at 3804.910 kb on - strand, within ABZR86_RS16850 at 3805.204 kb on + strand at 3805.204 kb on + strand at 3805.204 kb on + strand at 3805.204 kb on + strand at 3805.204 kb on + strand at 3805.204 kb on + strand at 3805.204 kb on + strand at 3805.205 kb on - strand at 3805.205 kb on - strand at 3805.205 kb on - strand at 3805.205 kb on - strand at 3805.205 kb on - strand at 3805.205 kb on - strand at 3805.264 kb on + strand, within ABZR86_RS16855 at 3805.265 kb on - strand, within ABZR86_RS16855 at 3805.315 kb on + strand, within ABZR86_RS16855 at 3805.316 kb on - strand, within ABZR86_RS16855 at 3805.316 kb on - strand, within ABZR86_RS16855 at 3805.414 kb on + strand, within ABZR86_RS16855 at 3805.414 kb on + strand, within ABZR86_RS16855 at 3805.414 kb on + strand, within ABZR86_RS16855 at 3805.414 kb on + strand, within ABZR86_RS16855 at 3805.415 kb on - strand, within ABZR86_RS16855 at 3805.415 kb on - strand, within ABZR86_RS16855 at 3805.415 kb on - strand, within ABZR86_RS16855 at 3805.441 kb on + strand, within ABZR86_RS16855 at 3805.442 kb on - strand, within ABZR86_RS16855 at 3805.442 kb on - strand, within ABZR86_RS16855 at 3805.693 kb on + strand at 3805.693 kb on + strand at 3805.693 kb on + strand at 3805.694 kb on - strand at 3805.694 kb on - strand at 3805.694 kb on - strand at 3806.314 kb on - strand, within ABZR86_RS16860 at 3806.322 kb on + strand, within ABZR86_RS16860 at 3806.323 kb on - strand, within ABZR86_RS16860 at 3806.349 kb on + strand, within ABZR86_RS16860 at 3806.349 kb on + strand, within ABZR86_RS16860 at 3806.349 kb on + strand, within ABZR86_RS16860 at 3806.350 kb on - strand, within ABZR86_RS16860 at 3806.350 kb on - strand, within ABZR86_RS16860 at 3806.350 kb on - strand, within ABZR86_RS16860 at 3806.362 kb on - strand, within ABZR86_RS16860 at 3806.362 kb on - strand, within ABZR86_RS16860 at 3806.362 kb on - strand, within ABZR86_RS16860 at 3806.558 kb on + strand, within ABZR86_RS16860 at 3806.559 kb on - strand, within ABZR86_RS16860 at 3806.679 kb on + strand, within ABZR86_RS16860 at 3806.680 kb on - strand, within ABZR86_RS16860 at 3807.161 kb on + strand at 3807.162 kb on - strand at 3807.373 kb on + strand, within ABZR86_RS16865 at 3807.373 kb on + strand, within ABZR86_RS16865 at 3807.373 kb on + strand, within ABZR86_RS16865 at 3807.374 kb on - strand, within ABZR86_RS16865 at 3807.374 kb on - strand, within ABZR86_RS16865 at 3807.374 kb on - strand, within ABZR86_RS16865 at 3807.382 kb on + strand, within ABZR86_RS16865 at 3807.382 kb on + strand, within ABZR86_RS16865 at 3807.382 kb on + strand, within ABZR86_RS16865 at 3807.382 kb on + strand, within ABZR86_RS16865 at 3807.383 kb on - strand, within ABZR86_RS16865 at 3807.383 kb on - strand, within ABZR86_RS16865 at 3807.433 kb on + strand, within ABZR86_RS16865
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.2 mg/ml remove 3,804,720 + ABZR86_RS16850 0.40 -0.3 3,804,721 - ABZR86_RS16850 0.41 -0.1 3,804,807 + ABZR86_RS16850 0.54 -0.9 3,804,807 + ABZR86_RS16850 0.54 +0.6 3,804,807 + ABZR86_RS16850 0.54 -0.0 3,804,807 + ABZR86_RS16850 0.54 -0.5 3,804,807 + ABZR86_RS16850 0.54 -0.2 3,804,807 + ABZR86_RS16850 0.54 +1.1 3,804,808 - ABZR86_RS16850 0.54 -1.1 3,804,808 - ABZR86_RS16850 0.54 -0.1 3,804,909 + ABZR86_RS16850 0.70 +0.3 3,804,909 + ABZR86_RS16850 0.70 -0.1 3,804,910 - ABZR86_RS16850 0.70 -0.5 3,804,910 - ABZR86_RS16850 0.70 +1.8 3,805,204 + +0.6 3,805,204 + -0.8 3,805,204 + -0.1 3,805,204 + +0.4 3,805,204 + -0.7 3,805,204 + -0.0 3,805,204 + +0.9 3,805,205 - -0.5 3,805,205 - +0.2 3,805,205 - +0.2 3,805,205 - +0.1 3,805,205 - -0.3 3,805,205 - +0.1 3,805,264 + ABZR86_RS16855 0.12 +0.5 3,805,265 - ABZR86_RS16855 0.12 -0.3 3,805,315 + ABZR86_RS16855 0.22 -0.2 3,805,316 - ABZR86_RS16855 0.22 +0.6 3,805,316 - ABZR86_RS16855 0.22 -0.5 3,805,414 + ABZR86_RS16855 0.42 -1.8 3,805,414 + ABZR86_RS16855 0.42 +0.2 3,805,414 + ABZR86_RS16855 0.42 -0.4 3,805,414 + ABZR86_RS16855 0.42 +0.9 3,805,415 - ABZR86_RS16855 0.42 -2.8 3,805,415 - ABZR86_RS16855 0.42 +1.2 3,805,415 - ABZR86_RS16855 0.42 -0.7 3,805,441 + ABZR86_RS16855 0.47 -2.4 3,805,442 - ABZR86_RS16855 0.47 +1.1 3,805,442 - ABZR86_RS16855 0.47 -0.0 3,805,693 + -0.3 3,805,693 + -0.4 3,805,693 + -0.3 3,805,694 - +0.5 3,805,694 - -0.0 3,805,694 - -0.5 3,806,314 - ABZR86_RS16860 0.45 +0.8 3,806,322 + ABZR86_RS16860 0.45 -0.1 3,806,323 - ABZR86_RS16860 0.45 +0.7 3,806,349 + ABZR86_RS16860 0.47 +1.6 3,806,349 + ABZR86_RS16860 0.47 -1.1 3,806,349 + ABZR86_RS16860 0.47 -1.3 3,806,350 - ABZR86_RS16860 0.47 -0.2 3,806,350 - ABZR86_RS16860 0.47 -1.4 3,806,350 - ABZR86_RS16860 0.47 +0.2 3,806,362 - ABZR86_RS16860 0.48 +2.7 3,806,362 - ABZR86_RS16860 0.48 +0.5 3,806,362 - ABZR86_RS16860 0.48 +0.5 3,806,558 + ABZR86_RS16860 0.62 -0.4 3,806,559 - ABZR86_RS16860 0.62 -0.0 3,806,679 + ABZR86_RS16860 0.71 -1.1 3,806,680 - ABZR86_RS16860 0.71 -0.4 3,807,161 + -0.1 3,807,162 - -1.2 3,807,373 + ABZR86_RS16865 0.57 +1.2 3,807,373 + ABZR86_RS16865 0.57 +0.7 3,807,373 + ABZR86_RS16865 0.57 +0.0 3,807,374 - ABZR86_RS16865 0.58 +0.1 3,807,374 - ABZR86_RS16865 0.58 +0.7 3,807,374 - ABZR86_RS16865 0.58 +0.9 3,807,382 + ABZR86_RS16865 0.60 -0.0 3,807,382 + ABZR86_RS16865 0.60 +0.5 3,807,382 + ABZR86_RS16865 0.60 +0.1 3,807,382 + ABZR86_RS16865 0.60 -0.3 3,807,383 - ABZR86_RS16865 0.60 +1.3 3,807,383 - ABZR86_RS16865 0.60 +0.7 3,807,433 + ABZR86_RS16865 0.75 +0.2
Or see this region's nucleotide sequence