Experiment: Fusidic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS09270 and ABZR86_RS09275 are separated by 119 nucleotides ABZR86_RS09275 and ABZR86_RS09280 are separated by 17 nucleotides ABZR86_RS09280 and ABZR86_RS09285 are separated by 28 nucleotides
ABZR86_RS09270: ABZR86_RS09270 - hypothetical protein, at 2,065,234 to 2,066,094
_RS09270
ABZR86_RS09275: ABZR86_RS09275 - aspartate/glutamate racemase family protein, at 2,066,214 to 2,066,906
_RS09275
ABZR86_RS09280: ABZR86_RS09280 - amino acid permease, at 2,066,924 to 2,068,285
_RS09280
ABZR86_RS09285: ABZR86_RS09285 - rhomboid family intramembrane serine protease, at 2,068,314 to 2,068,928
_RS09285
Position (kb)
2066
2067
2068
2069 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2065.945 kb on - strand, within ABZR86_RS09270 at 2065.945 kb on - strand, within ABZR86_RS09270 at 2066.018 kb on + strand at 2066.018 kb on + strand at 2066.021 kb on + strand at 2066.022 kb on - strand at 2066.022 kb on - strand at 2066.022 kb on - strand at 2066.022 kb on - strand at 2066.027 kb on + strand at 2066.028 kb on - strand at 2066.028 kb on - strand at 2066.060 kb on + strand at 2066.060 kb on + strand at 2066.060 kb on + strand at 2066.061 kb on - strand at 2066.061 kb on - strand at 2066.076 kb on - strand at 2066.076 kb on - strand at 2066.140 kb on + strand at 2066.140 kb on + strand at 2066.141 kb on - strand at 2066.141 kb on - strand at 2066.160 kb on - strand at 2066.215 kb on + strand at 2066.283 kb on - strand at 2066.283 kb on - strand at 2066.375 kb on - strand, within ABZR86_RS09275 at 2066.423 kb on - strand, within ABZR86_RS09275 at 2066.506 kb on + strand, within ABZR86_RS09275 at 2066.525 kb on + strand, within ABZR86_RS09275 at 2066.526 kb on - strand, within ABZR86_RS09275 at 2066.526 kb on - strand, within ABZR86_RS09275 at 2066.655 kb on - strand, within ABZR86_RS09275 at 2066.655 kb on - strand, within ABZR86_RS09275 at 2066.655 kb on - strand, within ABZR86_RS09275 at 2066.855 kb on - strand at 2067.018 kb on + strand at 2067.046 kb on - strand at 2067.046 kb on - strand at 2067.153 kb on + strand, within ABZR86_RS09280 at 2067.153 kb on + strand, within ABZR86_RS09280 at 2067.153 kb on + strand, within ABZR86_RS09280 at 2067.190 kb on + strand, within ABZR86_RS09280 at 2067.191 kb on - strand, within ABZR86_RS09280 at 2067.243 kb on + strand, within ABZR86_RS09280 at 2067.243 kb on + strand, within ABZR86_RS09280 at 2067.244 kb on - strand, within ABZR86_RS09280 at 2067.244 kb on - strand, within ABZR86_RS09280 at 2067.244 kb on - strand, within ABZR86_RS09280 at 2067.244 kb on - strand, within ABZR86_RS09280 at 2067.363 kb on + strand, within ABZR86_RS09280 at 2067.364 kb on - strand, within ABZR86_RS09280 at 2067.364 kb on - strand, within ABZR86_RS09280 at 2067.364 kb on - strand, within ABZR86_RS09280 at 2067.954 kb on + strand, within ABZR86_RS09280 at 2067.954 kb on + strand, within ABZR86_RS09280 at 2067.954 kb on + strand, within ABZR86_RS09280 at 2067.954 kb on + strand, within ABZR86_RS09280 at 2067.955 kb on - strand, within ABZR86_RS09280 at 2067.955 kb on - strand, within ABZR86_RS09280 at 2067.955 kb on - strand, within ABZR86_RS09280 at 2067.955 kb on - strand, within ABZR86_RS09280 at 2068.027 kb on - strand, within ABZR86_RS09280 at 2068.048 kb on - strand, within ABZR86_RS09280 at 2068.059 kb on + strand, within ABZR86_RS09280 at 2068.059 kb on + strand, within ABZR86_RS09280 at 2068.060 kb on - strand, within ABZR86_RS09280 at 2068.060 kb on - strand, within ABZR86_RS09280 at 2068.149 kb on + strand at 2068.246 kb on + strand at 2068.247 kb on - strand at 2068.667 kb on - strand, within ABZR86_RS09285 at 2068.667 kb on - strand, within ABZR86_RS09285 at 2068.669 kb on + strand, within ABZR86_RS09285 at 2068.669 kb on + strand, within ABZR86_RS09285 at 2068.669 kb on + strand, within ABZR86_RS09285 at 2068.669 kb on + strand, within ABZR86_RS09285 at 2068.670 kb on - strand, within ABZR86_RS09285 at 2068.670 kb on - strand, within ABZR86_RS09285 at 2068.678 kb on + strand, within ABZR86_RS09285 at 2068.678 kb on + strand, within ABZR86_RS09285 at 2068.678 kb on + strand, within ABZR86_RS09285 at 2068.732 kb on + strand, within ABZR86_RS09285 at 2068.733 kb on - strand, within ABZR86_RS09285 at 2068.733 kb on - strand, within ABZR86_RS09285 at 2068.733 kb on - strand, within ABZR86_RS09285 at 2068.804 kb on + strand, within ABZR86_RS09285 at 2068.804 kb on + strand, within ABZR86_RS09285 at 2069.132 kb on - strand at 2069.132 kb on - strand at 2069.133 kb on + strand at 2069.133 kb on + strand at 2069.134 kb on - strand at 2069.134 kb on - strand at 2069.134 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.2 mg/ml remove 2,065,945 - ABZR86_RS09270 0.83 +0.2 2,065,945 - ABZR86_RS09270 0.83 +0.2 2,066,018 + +0.3 2,066,018 + +0.5 2,066,021 + +1.3 2,066,022 - -0.6 2,066,022 - +0.3 2,066,022 - +0.3 2,066,022 - -0.1 2,066,027 + +1.2 2,066,028 - -0.7 2,066,028 - +0.5 2,066,060 + +0.5 2,066,060 + -1.0 2,066,060 + -0.8 2,066,061 - +0.5 2,066,061 - +0.2 2,066,076 - +0.6 2,066,076 - -0.5 2,066,140 + +2.7 2,066,140 + -0.8 2,066,141 - +0.2 2,066,141 - -0.6 2,066,160 - +0.3 2,066,215 + +0.3 2,066,283 - +0.0 2,066,283 - +0.2 2,066,375 - ABZR86_RS09275 0.23 +0.2 2,066,423 - ABZR86_RS09275 0.30 -1.6 2,066,506 + ABZR86_RS09275 0.42 -1.0 2,066,525 + ABZR86_RS09275 0.45 +0.7 2,066,526 - ABZR86_RS09275 0.45 -0.1 2,066,526 - ABZR86_RS09275 0.45 +0.7 2,066,655 - ABZR86_RS09275 0.64 -0.3 2,066,655 - ABZR86_RS09275 0.64 +0.3 2,066,655 - ABZR86_RS09275 0.64 +0.4 2,066,855 - -0.5 2,067,018 + -0.0 2,067,046 - +0.7 2,067,046 - -0.6 2,067,153 + ABZR86_RS09280 0.17 -1.2 2,067,153 + ABZR86_RS09280 0.17 -0.3 2,067,153 + ABZR86_RS09280 0.17 +0.0 2,067,190 + ABZR86_RS09280 0.20 +0.0 2,067,191 - ABZR86_RS09280 0.20 -0.1 2,067,243 + ABZR86_RS09280 0.23 +0.3 2,067,243 + ABZR86_RS09280 0.23 -0.1 2,067,244 - ABZR86_RS09280 0.23 +0.9 2,067,244 - ABZR86_RS09280 0.23 -0.4 2,067,244 - ABZR86_RS09280 0.23 -0.9 2,067,244 - ABZR86_RS09280 0.23 -0.8 2,067,363 + ABZR86_RS09280 0.32 +1.4 2,067,364 - ABZR86_RS09280 0.32 -1.1 2,067,364 - ABZR86_RS09280 0.32 +0.4 2,067,364 - ABZR86_RS09280 0.32 +0.1 2,067,954 + ABZR86_RS09280 0.76 +0.3 2,067,954 + ABZR86_RS09280 0.76 +0.3 2,067,954 + ABZR86_RS09280 0.76 +0.2 2,067,954 + ABZR86_RS09280 0.76 +0.2 2,067,955 - ABZR86_RS09280 0.76 -0.2 2,067,955 - ABZR86_RS09280 0.76 -0.1 2,067,955 - ABZR86_RS09280 0.76 +0.6 2,067,955 - ABZR86_RS09280 0.76 -0.4 2,068,027 - ABZR86_RS09280 0.81 -0.1 2,068,048 - ABZR86_RS09280 0.83 -1.1 2,068,059 + ABZR86_RS09280 0.83 +1.0 2,068,059 + ABZR86_RS09280 0.83 -0.2 2,068,060 - ABZR86_RS09280 0.83 -0.4 2,068,060 - ABZR86_RS09280 0.83 -0.5 2,068,149 + +0.2 2,068,246 + -0.4 2,068,247 - +1.4 2,068,667 - ABZR86_RS09285 0.57 -0.1 2,068,667 - ABZR86_RS09285 0.57 +0.1 2,068,669 + ABZR86_RS09285 0.58 -0.5 2,068,669 + ABZR86_RS09285 0.58 +0.0 2,068,669 + ABZR86_RS09285 0.58 +0.0 2,068,669 + ABZR86_RS09285 0.58 -0.0 2,068,670 - ABZR86_RS09285 0.58 -0.0 2,068,670 - ABZR86_RS09285 0.58 +0.5 2,068,678 + ABZR86_RS09285 0.59 -2.8 2,068,678 + ABZR86_RS09285 0.59 -0.2 2,068,678 + ABZR86_RS09285 0.59 -0.0 2,068,732 + ABZR86_RS09285 0.68 +0.1 2,068,733 - ABZR86_RS09285 0.68 -0.0 2,068,733 - ABZR86_RS09285 0.68 -0.6 2,068,733 - ABZR86_RS09285 0.68 +0.6 2,068,804 + ABZR86_RS09285 0.80 -0.6 2,068,804 + ABZR86_RS09285 0.80 -0.8 2,069,132 - -0.1 2,069,132 - +1.0 2,069,133 + +0.1 2,069,133 + -1.4 2,069,134 - +0.9 2,069,134 - +0.2 2,069,134 - -0.1
Or see this region's nucleotide sequence