Experiment: Fusidic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS06005 and ABZR86_RS06010 are separated by 18 nucleotides ABZR86_RS06010 and ABZR86_RS06015 are separated by 162 nucleotides ABZR86_RS06015 and ABZR86_RS06020 overlap by 1 nucleotides ABZR86_RS06020 and ABZR86_RS06025 are separated by 146 nucleotides
ABZR86_RS06005: ABZR86_RS06005 - polyribonucleotide nucleotidyltransferase, at 1,348,570 to 1,350,669
_RS06005
ABZR86_RS06010: ABZR86_RS06010 - 30S ribosomal protein S15, at 1,350,688 to 1,350,957
_RS06010
ABZR86_RS06015: ABZR86_RS06015 - tRNA pseudouridine(55) synthase TruB, at 1,351,120 to 1,352,049
_RS06015
ABZR86_RS06020: ABZR86_RS06020 - 30S ribosome-binding factor RbfA, at 1,352,049 to 1,352,447
_RS06020
ABZR86_RS06025: ABZR86_RS06025 - translation initiation factor IF-2, at 1,352,594 to 1,355,401
_RS06025
Position (kb)
1351
1352
1353 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1351.235 kb on + strand, within ABZR86_RS06015 at 1351.236 kb on - strand, within ABZR86_RS06015 at 1351.517 kb on + strand, within ABZR86_RS06015 at 1351.517 kb on + strand, within ABZR86_RS06015 at 1351.517 kb on + strand, within ABZR86_RS06015 at 1351.517 kb on + strand, within ABZR86_RS06015 at 1351.517 kb on + strand, within ABZR86_RS06015 at 1351.517 kb on + strand, within ABZR86_RS06015 at 1351.517 kb on + strand, within ABZR86_RS06015 at 1351.517 kb on + strand, within ABZR86_RS06015 at 1351.517 kb on + strand, within ABZR86_RS06015 at 1351.518 kb on - strand, within ABZR86_RS06015 at 1351.518 kb on - strand, within ABZR86_RS06015 at 1351.518 kb on - strand, within ABZR86_RS06015 at 1351.518 kb on - strand, within ABZR86_RS06015 at 1351.518 kb on - strand, within ABZR86_RS06015 at 1351.518 kb on - strand, within ABZR86_RS06015 at 1351.643 kb on + strand, within ABZR86_RS06015 at 1351.643 kb on + strand, within ABZR86_RS06015 at 1351.644 kb on - strand, within ABZR86_RS06015 at 1351.644 kb on - strand, within ABZR86_RS06015 at 1351.644 kb on - strand, within ABZR86_RS06015 at 1351.677 kb on - strand, within ABZR86_RS06015 at 1351.677 kb on - strand, within ABZR86_RS06015 at 1351.677 kb on - strand, within ABZR86_RS06015 at 1351.677 kb on - strand, within ABZR86_RS06015 at 1352.514 kb on - strand at 1352.560 kb on + strand at 1352.595 kb on + strand at 1352.595 kb on + strand at 1352.602 kb on + strand at 1352.602 kb on + strand at 1352.603 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.2 mg/ml remove 1,351,235 + ABZR86_RS06015 0.12 +1.6 1,351,236 - ABZR86_RS06015 0.12 +0.0 1,351,517 + ABZR86_RS06015 0.43 -0.5 1,351,517 + ABZR86_RS06015 0.43 -0.3 1,351,517 + ABZR86_RS06015 0.43 -2.4 1,351,517 + ABZR86_RS06015 0.43 +0.2 1,351,517 + ABZR86_RS06015 0.43 -0.2 1,351,517 + ABZR86_RS06015 0.43 -0.7 1,351,517 + ABZR86_RS06015 0.43 +1.1 1,351,517 + ABZR86_RS06015 0.43 -1.2 1,351,517 + ABZR86_RS06015 0.43 -1.3 1,351,518 - ABZR86_RS06015 0.43 -0.8 1,351,518 - ABZR86_RS06015 0.43 +0.5 1,351,518 - ABZR86_RS06015 0.43 +0.4 1,351,518 - ABZR86_RS06015 0.43 +0.2 1,351,518 - ABZR86_RS06015 0.43 -0.2 1,351,518 - ABZR86_RS06015 0.43 -0.6 1,351,643 + ABZR86_RS06015 0.56 +0.3 1,351,643 + ABZR86_RS06015 0.56 -0.3 1,351,644 - ABZR86_RS06015 0.56 -1.2 1,351,644 - ABZR86_RS06015 0.56 +0.1 1,351,644 - ABZR86_RS06015 0.56 -0.1 1,351,677 - ABZR86_RS06015 0.60 -2.8 1,351,677 - ABZR86_RS06015 0.60 +0.6 1,351,677 - ABZR86_RS06015 0.60 +0.6 1,351,677 - ABZR86_RS06015 0.60 -0.8 1,352,514 - +0.1 1,352,560 + -1.8 1,352,595 + +0.2 1,352,595 + -0.6 1,352,602 + +1.0 1,352,602 + +1.2 1,352,603 - +1.0
Or see this region's nucleotide sequence