Experiment: Fusidic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS05215 and ABZR86_RS05220 are separated by 34 nucleotides ABZR86_RS05220 and ABZR86_RS05225 are separated by 3 nucleotides ABZR86_RS05225 and ABZR86_RS05230 are separated by 50 nucleotides ABZR86_RS05230 and ABZR86_RS05235 are separated by 92 nucleotides ABZR86_RS05235 and ABZR86_RS05240 are separated by 43 nucleotides
ABZR86_RS05215: ABZR86_RS05215 - O-acetyl-ADP-ribose deacetylase, at 1,177,671 to 1,178,201
_RS05215
ABZR86_RS05220: ABZR86_RS05220 - hypothetical protein, at 1,178,236 to 1,178,544
_RS05220
ABZR86_RS05225: ABZR86_RS05225 - hypothetical protein, at 1,178,548 to 1,178,754
_RS05225
ABZR86_RS05230: ABZR86_RS05230 - TetR family transcriptional regulator, at 1,178,805 to 1,179,437
_RS05230
ABZR86_RS05235: ABZR86_RS05235 - O-methyltransferase, at 1,179,530 to 1,180,186
_RS05235
ABZR86_RS05240: ABZR86_RS05240 - hypothetical protein, at 1,180,230 to 1,180,463
_RS05240
Position (kb) 1178
1179
1180 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1177.837 kb on + strand, within ABZR86_RS05215 at 1177.838 kb on - strand, within ABZR86_RS05215 at 1177.838 kb on - strand, within ABZR86_RS05215 at 1177.838 kb on - strand, within ABZR86_RS05215 at 1178.212 kb on + strand at 1178.262 kb on - strand at 1178.262 kb on - strand at 1178.267 kb on - strand, within ABZR86_RS05220 at 1178.315 kb on - strand, within ABZR86_RS05220 at 1178.315 kb on - strand, within ABZR86_RS05220 at 1178.369 kb on - strand, within ABZR86_RS05220 at 1178.550 kb on - strand at 1178.584 kb on - strand, within ABZR86_RS05225 at 1178.790 kb on + strand at 1178.791 kb on - strand at 1178.792 kb on + strand at 1178.792 kb on + strand at 1178.793 kb on - strand at 1178.798 kb on + strand at 1178.799 kb on - strand at 1178.799 kb on - strand at 1178.799 kb on - strand at 1178.830 kb on + strand at 1178.830 kb on + strand at 1178.863 kb on + strand at 1178.863 kb on + strand at 1179.027 kb on + strand, within ABZR86_RS05230 at 1179.253 kb on + strand, within ABZR86_RS05230 at 1179.253 kb on + strand, within ABZR86_RS05230 at 1179.253 kb on + strand, within ABZR86_RS05230 at 1179.254 kb on - strand, within ABZR86_RS05230 at 1179.254 kb on - strand, within ABZR86_RS05230 at 1179.260 kb on - strand, within ABZR86_RS05230 at 1179.260 kb on - strand, within ABZR86_RS05230 at 1179.503 kb on + strand at 1179.542 kb on - strand at 1179.547 kb on + strand at 1179.548 kb on - strand at 1179.665 kb on + strand, within ABZR86_RS05235 at 1179.665 kb on + strand, within ABZR86_RS05235 at 1179.734 kb on + strand, within ABZR86_RS05235 at 1179.734 kb on + strand, within ABZR86_RS05235 at 1179.734 kb on + strand, within ABZR86_RS05235 at 1179.734 kb on + strand, within ABZR86_RS05235 at 1179.734 kb on + strand, within ABZR86_RS05235 at 1179.735 kb on - strand, within ABZR86_RS05235 at 1179.735 kb on - strand, within ABZR86_RS05235 at 1180.133 kb on + strand at 1180.254 kb on - strand, within ABZR86_RS05240 at 1180.348 kb on - strand, within ABZR86_RS05240 at 1180.348 kb on - strand, within ABZR86_RS05240 at 1180.348 kb on - strand, within ABZR86_RS05240
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.2 mg/ml remove 1,177,837 + ABZR86_RS05215 0.31 -0.1 1,177,838 - ABZR86_RS05215 0.31 +0.3 1,177,838 - ABZR86_RS05215 0.31 -0.1 1,177,838 - ABZR86_RS05215 0.31 -0.5 1,178,212 + -0.5 1,178,262 - -0.1 1,178,262 - +0.6 1,178,267 - ABZR86_RS05220 0.10 +0.1 1,178,315 - ABZR86_RS05220 0.26 -0.3 1,178,315 - ABZR86_RS05220 0.26 +0.3 1,178,369 - ABZR86_RS05220 0.43 +0.4 1,178,550 - -0.8 1,178,584 - ABZR86_RS05225 0.17 +0.6 1,178,790 + +0.1 1,178,791 - +0.1 1,178,792 + -0.5 1,178,792 + -1.1 1,178,793 - +0.0 1,178,798 + -0.1 1,178,799 - +0.3 1,178,799 - -0.1 1,178,799 - -0.7 1,178,830 + -1.2 1,178,830 + -2.2 1,178,863 + +0.1 1,178,863 + +0.2 1,179,027 + ABZR86_RS05230 0.35 +1.5 1,179,253 + ABZR86_RS05230 0.71 -0.4 1,179,253 + ABZR86_RS05230 0.71 +0.1 1,179,253 + ABZR86_RS05230 0.71 +0.0 1,179,254 - ABZR86_RS05230 0.71 +1.0 1,179,254 - ABZR86_RS05230 0.71 -0.5 1,179,260 - ABZR86_RS05230 0.72 -1.2 1,179,260 - ABZR86_RS05230 0.72 +0.1 1,179,503 + +0.6 1,179,542 - -0.8 1,179,547 + -0.5 1,179,548 - +2.3 1,179,665 + ABZR86_RS05235 0.21 +0.1 1,179,665 + ABZR86_RS05235 0.21 -1.1 1,179,734 + ABZR86_RS05235 0.31 -0.3 1,179,734 + ABZR86_RS05235 0.31 -0.1 1,179,734 + ABZR86_RS05235 0.31 -1.2 1,179,734 + ABZR86_RS05235 0.31 +0.0 1,179,734 + ABZR86_RS05235 0.31 +0.1 1,179,735 - ABZR86_RS05235 0.31 +0.6 1,179,735 - ABZR86_RS05235 0.31 +0.5 1,180,133 + -0.8 1,180,254 - ABZR86_RS05240 0.10 -2.1 1,180,348 - ABZR86_RS05240 0.50 +0.7 1,180,348 - ABZR86_RS05240 0.50 -2.0 1,180,348 - ABZR86_RS05240 0.50 -0.8
Or see this region's nucleotide sequence