Experiment: Fusidic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS00830 and ABZR86_RS00835 are separated by 5 nucleotides ABZR86_RS00835 and ABZR86_RS00840 are separated by 50 nucleotides
ABZR86_RS00830: ABZR86_RS00830 - glucose-6-phosphate isomerase, at 160,388 to 162,025
_RS00830
ABZR86_RS00835: ABZR86_RS00835 - tRNA epoxyqueuosine(34) reductase QueG, at 162,031 to 163,104
_RS00835
ABZR86_RS00840: ABZR86_RS00840 - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 163,155 to 164,639
_RS00840
Position (kb)
162
163
164 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 161.060 kb on + strand, within ABZR86_RS00830 at 161.061 kb on - strand, within ABZR86_RS00830 at 161.156 kb on + strand, within ABZR86_RS00830 at 161.156 kb on + strand, within ABZR86_RS00830 at 161.157 kb on - strand, within ABZR86_RS00830 at 161.340 kb on - strand, within ABZR86_RS00830 at 161.340 kb on - strand, within ABZR86_RS00830 at 161.387 kb on + strand, within ABZR86_RS00830 at 161.417 kb on + strand, within ABZR86_RS00830 at 161.417 kb on + strand, within ABZR86_RS00830 at 161.418 kb on - strand, within ABZR86_RS00830 at 161.537 kb on + strand, within ABZR86_RS00830 at 161.537 kb on + strand, within ABZR86_RS00830 at 161.537 kb on + strand, within ABZR86_RS00830 at 161.537 kb on + strand, within ABZR86_RS00830 at 161.538 kb on - strand, within ABZR86_RS00830 at 161.538 kb on - strand, within ABZR86_RS00830 at 161.538 kb on - strand, within ABZR86_RS00830 at 161.538 kb on - strand, within ABZR86_RS00830 at 161.566 kb on + strand, within ABZR86_RS00830 at 161.834 kb on + strand, within ABZR86_RS00830 at 161.835 kb on - strand, within ABZR86_RS00830 at 162.032 kb on + strand at 162.032 kb on + strand at 162.033 kb on - strand at 162.033 kb on - strand at 162.162 kb on + strand, within ABZR86_RS00835 at 162.163 kb on - strand, within ABZR86_RS00835 at 162.425 kb on + strand, within ABZR86_RS00835 at 162.426 kb on - strand, within ABZR86_RS00835 at 162.426 kb on - strand, within ABZR86_RS00835 at 162.426 kb on - strand, within ABZR86_RS00835 at 162.456 kb on - strand, within ABZR86_RS00835 at 162.557 kb on + strand, within ABZR86_RS00835 at 162.557 kb on + strand, within ABZR86_RS00835 at 162.557 kb on + strand, within ABZR86_RS00835 at 162.557 kb on + strand, within ABZR86_RS00835 at 162.558 kb on - strand, within ABZR86_RS00835 at 162.558 kb on - strand, within ABZR86_RS00835 at 162.578 kb on + strand, within ABZR86_RS00835 at 162.578 kb on + strand, within ABZR86_RS00835 at 162.579 kb on - strand, within ABZR86_RS00835 at 162.579 kb on - strand, within ABZR86_RS00835 at 162.579 kb on - strand, within ABZR86_RS00835 at 162.579 kb on - strand, within ABZR86_RS00835 at 162.579 kb on - strand, within ABZR86_RS00835 at 162.761 kb on + strand, within ABZR86_RS00835 at 162.761 kb on + strand, within ABZR86_RS00835 at 162.762 kb on - strand, within ABZR86_RS00835 at 162.791 kb on + strand, within ABZR86_RS00835 at 162.848 kb on + strand, within ABZR86_RS00835 at 162.848 kb on + strand, within ABZR86_RS00835 at 162.848 kb on + strand, within ABZR86_RS00835 at 162.848 kb on + strand, within ABZR86_RS00835 at 162.848 kb on + strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.849 kb on - strand, within ABZR86_RS00835 at 162.932 kb on + strand, within ABZR86_RS00835 at 162.932 kb on + strand, within ABZR86_RS00835 at 162.932 kb on + strand, within ABZR86_RS00835 at 162.932 kb on + strand, within ABZR86_RS00835 at 162.932 kb on + strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 162.933 kb on - strand, within ABZR86_RS00835 at 163.116 kb on + strand at 163.116 kb on + strand at 163.117 kb on - strand at 163.117 kb on - strand at 163.117 kb on - strand at 163.142 kb on + strand at 163.142 kb on + strand at 163.143 kb on - strand at 163.144 kb on + strand at 163.145 kb on - strand at 163.185 kb on + strand at 163.185 kb on + strand at 163.329 kb on + strand, within ABZR86_RS00840 at 163.542 kb on + strand, within ABZR86_RS00840 at 163.542 kb on + strand, within ABZR86_RS00840
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.2 mg/ml remove 161,060 + ABZR86_RS00830 0.41 +0.1 161,061 - ABZR86_RS00830 0.41 +0.1 161,156 + ABZR86_RS00830 0.47 +0.3 161,156 + ABZR86_RS00830 0.47 +0.8 161,157 - ABZR86_RS00830 0.47 -0.3 161,340 - ABZR86_RS00830 0.58 +0.1 161,340 - ABZR86_RS00830 0.58 +0.1 161,387 + ABZR86_RS00830 0.61 -0.0 161,417 + ABZR86_RS00830 0.63 -0.4 161,417 + ABZR86_RS00830 0.63 +0.5 161,418 - ABZR86_RS00830 0.63 -0.1 161,537 + ABZR86_RS00830 0.70 -0.1 161,537 + ABZR86_RS00830 0.70 +0.2 161,537 + ABZR86_RS00830 0.70 -0.1 161,537 + ABZR86_RS00830 0.70 -0.5 161,538 - ABZR86_RS00830 0.70 -0.7 161,538 - ABZR86_RS00830 0.70 +1.4 161,538 - ABZR86_RS00830 0.70 -0.1 161,538 - ABZR86_RS00830 0.70 -1.4 161,566 + ABZR86_RS00830 0.72 -1.4 161,834 + ABZR86_RS00830 0.88 +0.1 161,835 - ABZR86_RS00830 0.88 -0.0 162,032 + -0.3 162,032 + +0.1 162,033 - +0.1 162,033 - +0.3 162,162 + ABZR86_RS00835 0.12 -0.8 162,163 - ABZR86_RS00835 0.12 +0.6 162,425 + ABZR86_RS00835 0.37 -0.1 162,426 - ABZR86_RS00835 0.37 -0.5 162,426 - ABZR86_RS00835 0.37 +0.8 162,426 - ABZR86_RS00835 0.37 +0.7 162,456 - ABZR86_RS00835 0.40 +0.2 162,557 + ABZR86_RS00835 0.49 +0.7 162,557 + ABZR86_RS00835 0.49 -0.7 162,557 + ABZR86_RS00835 0.49 -0.2 162,557 + ABZR86_RS00835 0.49 +0.3 162,558 - ABZR86_RS00835 0.49 -0.3 162,558 - ABZR86_RS00835 0.49 +0.5 162,578 + ABZR86_RS00835 0.51 +0.3 162,578 + ABZR86_RS00835 0.51 +0.2 162,579 - ABZR86_RS00835 0.51 +0.9 162,579 - ABZR86_RS00835 0.51 -0.5 162,579 - ABZR86_RS00835 0.51 +1.3 162,579 - ABZR86_RS00835 0.51 -0.3 162,579 - ABZR86_RS00835 0.51 +0.2 162,761 + ABZR86_RS00835 0.68 +0.7 162,761 + ABZR86_RS00835 0.68 +0.8 162,762 - ABZR86_RS00835 0.68 +0.2 162,791 + ABZR86_RS00835 0.71 -0.2 162,848 + ABZR86_RS00835 0.76 -2.6 162,848 + ABZR86_RS00835 0.76 +0.2 162,848 + ABZR86_RS00835 0.76 +0.7 162,848 + ABZR86_RS00835 0.76 +0.3 162,848 + ABZR86_RS00835 0.76 +0.8 162,849 - ABZR86_RS00835 0.76 -0.5 162,849 - ABZR86_RS00835 0.76 -0.9 162,849 - ABZR86_RS00835 0.76 +0.5 162,849 - ABZR86_RS00835 0.76 +0.1 162,849 - ABZR86_RS00835 0.76 +0.2 162,849 - ABZR86_RS00835 0.76 -0.4 162,849 - ABZR86_RS00835 0.76 -2.3 162,849 - ABZR86_RS00835 0.76 -0.1 162,849 - ABZR86_RS00835 0.76 -0.2 162,849 - ABZR86_RS00835 0.76 -3.3 162,849 - ABZR86_RS00835 0.76 +0.4 162,932 + ABZR86_RS00835 0.84 -0.4 162,932 + ABZR86_RS00835 0.84 +1.1 162,932 + ABZR86_RS00835 0.84 +1.4 162,932 + ABZR86_RS00835 0.84 +0.5 162,932 + ABZR86_RS00835 0.84 -0.1 162,933 - ABZR86_RS00835 0.84 +0.3 162,933 - ABZR86_RS00835 0.84 +1.1 162,933 - ABZR86_RS00835 0.84 +0.4 162,933 - ABZR86_RS00835 0.84 +0.1 162,933 - ABZR86_RS00835 0.84 -0.6 162,933 - ABZR86_RS00835 0.84 -0.1 162,933 - ABZR86_RS00835 0.84 +0.8 162,933 - ABZR86_RS00835 0.84 +0.2 162,933 - ABZR86_RS00835 0.84 -0.9 162,933 - ABZR86_RS00835 0.84 -1.4 162,933 - ABZR86_RS00835 0.84 +0.4 163,116 + +0.4 163,116 + +0.3 163,117 - -0.1 163,117 - -0.0 163,117 - -0.4 163,142 + +0.0 163,142 + +0.4 163,143 - -0.1 163,144 + -0.6 163,145 - +0.4 163,185 + +0.8 163,185 + +0.2 163,329 + ABZR86_RS00840 0.12 -0.4 163,542 + ABZR86_RS00840 0.26 +1.1 163,542 + ABZR86_RS00840 0.26 -0.6
Or see this region's nucleotide sequence