Experiment: BHIS with Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4714 and BT4715 are separated by 105 nucleotides BT4715 and BT4716 are separated by 136 nucleotides
BT4714: BT4714 - periplasmic beta-glucosidase precursor, xylosidase/arabinosidase (NCBI ptt file), at 6,188,192 to 6,190,501
BT4714
BT4715: BT4715 - non-specific DNA-binding protein Dps (NCBI ptt file), at 6,190,607 to 6,191,083
BT4715
BT4716: BT4716 - redox-sensitive transcriptional activator (NCBI ptt file), at 6,191,220 to 6,192,146
BT4716
Position (kb)
6190
6191
6192 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6189.693 kb on + strand, within BT4714 at 6189.728 kb on + strand, within BT4714 at 6189.729 kb on - strand, within BT4714 at 6189.806 kb on - strand, within BT4714 at 6189.881 kb on + strand, within BT4714 at 6189.882 kb on - strand, within BT4714 at 6189.971 kb on + strand, within BT4714 at 6189.972 kb on - strand, within BT4714 at 6190.054 kb on - strand, within BT4714 at 6190.066 kb on + strand, within BT4714 at 6190.095 kb on - strand, within BT4714 at 6190.148 kb on - strand, within BT4714 at 6190.277 kb on + strand at 6190.308 kb on + strand at 6190.308 kb on + strand at 6190.309 kb on - strand at 6190.464 kb on + strand at 6190.465 kb on - strand at 6190.465 kb on - strand at 6190.517 kb on - strand at 6190.536 kb on - strand at 6190.619 kb on - strand at 6190.621 kb on - strand at 6190.750 kb on + strand, within BT4715 at 6190.750 kb on + strand, within BT4715 at 6190.757 kb on + strand, within BT4715 at 6190.758 kb on - strand, within BT4715 at 6190.760 kb on + strand, within BT4715 at 6190.790 kb on + strand, within BT4715 at 6190.818 kb on - strand, within BT4715 at 6190.827 kb on + strand, within BT4715 at 6190.911 kb on + strand, within BT4715 at 6190.911 kb on + strand, within BT4715 at 6190.912 kb on - strand, within BT4715 at 6190.960 kb on + strand, within BT4715 at 6190.989 kb on + strand, within BT4715 at 6190.989 kb on + strand, within BT4715 at 6191.033 kb on + strand, within BT4715 at 6191.128 kb on + strand at 6191.129 kb on - strand at 6191.241 kb on + strand at 6191.246 kb on - strand at 6191.246 kb on - strand at 6191.246 kb on - strand at 6191.321 kb on + strand, within BT4716 at 6191.510 kb on - strand, within BT4716 at 6191.522 kb on - strand, within BT4716 at 6191.530 kb on - strand, within BT4716 at 6191.533 kb on + strand, within BT4716 at 6191.584 kb on + strand, within BT4716 at 6191.585 kb on - strand, within BT4716 at 6191.585 kb on - strand, within BT4716 at 6191.613 kb on - strand, within BT4716 at 6191.639 kb on + strand, within BT4716 at 6191.655 kb on - strand, within BT4716 at 6191.722 kb on - strand, within BT4716 at 6191.726 kb on - strand, within BT4716 at 6191.762 kb on - strand, within BT4716 at 6191.797 kb on + strand, within BT4716 at 6191.833 kb on + strand, within BT4716 at 6191.892 kb on - strand, within BT4716 at 6191.915 kb on - strand, within BT4716 at 6192.014 kb on + strand, within BT4716 at 6192.051 kb on - strand, within BT4716 at 6192.073 kb on + strand at 6192.073 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Paraquat dichloride 0.1 mg/ml remove 6,189,693 + BT4714 0.65 -1.5 6,189,728 + BT4714 0.66 +0.9 6,189,729 - BT4714 0.67 -1.2 6,189,806 - BT4714 0.70 +0.9 6,189,881 + BT4714 0.73 -0.4 6,189,882 - BT4714 0.73 -2.1 6,189,971 + BT4714 0.77 +0.1 6,189,972 - BT4714 0.77 -3.6 6,190,054 - BT4714 0.81 -0.8 6,190,066 + BT4714 0.81 -3.6 6,190,095 - BT4714 0.82 -0.0 6,190,148 - BT4714 0.85 +1.3 6,190,277 + -2.0 6,190,308 + +1.5 6,190,308 + -1.7 6,190,309 - -1.3 6,190,464 + -3.1 6,190,465 - -2.2 6,190,465 - +0.2 6,190,517 - +2.1 6,190,536 - +1.2 6,190,619 - -3.1 6,190,621 - -1.6 6,190,750 + BT4715 0.30 -1.7 6,190,750 + BT4715 0.30 +3.5 6,190,757 + BT4715 0.31 -1.1 6,190,758 - BT4715 0.32 -1.1 6,190,760 + BT4715 0.32 -0.5 6,190,790 + BT4715 0.38 +1.1 6,190,818 - BT4715 0.44 -1.6 6,190,827 + BT4715 0.46 -2.0 6,190,911 + BT4715 0.64 -0.1 6,190,911 + BT4715 0.64 +0.1 6,190,912 - BT4715 0.64 -0.6 6,190,960 + BT4715 0.74 -1.8 6,190,989 + BT4715 0.80 -1.7 6,190,989 + BT4715 0.80 +1.0 6,191,033 + BT4715 0.89 -0.2 6,191,128 + -0.5 6,191,129 - -0.3 6,191,241 + -3.4 6,191,246 - +0.1 6,191,246 - +0.1 6,191,246 - +0.1 6,191,321 + BT4716 0.11 -0.4 6,191,510 - BT4716 0.31 -1.7 6,191,522 - BT4716 0.33 +0.9 6,191,530 - BT4716 0.33 -2.3 6,191,533 + BT4716 0.34 +1.7 6,191,584 + BT4716 0.39 -3.0 6,191,585 - BT4716 0.39 +0.9 6,191,585 - BT4716 0.39 -1.4 6,191,613 - BT4716 0.42 -0.5 6,191,639 + BT4716 0.45 -1.9 6,191,655 - BT4716 0.47 -2.3 6,191,722 - BT4716 0.54 -0.6 6,191,726 - BT4716 0.55 +0.6 6,191,762 - BT4716 0.58 -2.6 6,191,797 + BT4716 0.62 -2.5 6,191,833 + BT4716 0.66 -3.0 6,191,892 - BT4716 0.72 -1.8 6,191,915 - BT4716 0.75 -2.1 6,192,014 + BT4716 0.86 -1.1 6,192,051 - BT4716 0.90 +0.4 6,192,073 + -0.4 6,192,073 + -2.5
Or see this region's nucleotide sequence