Experiment: BHIS with Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4353 and BT4354 are separated by 116 nucleotides BT4354 and BT4355 are separated by 389 nucleotides
BT4353: BT4353 - valyl-tRNA synthetase (NCBI ptt file), at 5,734,500 to 5,737,139
BT4353
BT4354: BT4354 - two-component system sensor histidine kinase (NCBI ptt file), at 5,737,256 to 5,739,151
BT4354
BT4355: BT4355 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 5,739,541 to 5,740,116
BT4355
Position (kb)
5737
5738
5739
5740 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5737.149 kb on - strand at 5737.152 kb on + strand at 5737.162 kb on + strand at 5737.220 kb on + strand at 5737.276 kb on - strand at 5737.409 kb on + strand at 5737.415 kb on + strand at 5737.446 kb on + strand, within BT4354 at 5737.633 kb on - strand, within BT4354 at 5737.766 kb on + strand, within BT4354 at 5737.793 kb on + strand, within BT4354 at 5737.794 kb on - strand, within BT4354 at 5737.795 kb on + strand, within BT4354 at 5738.032 kb on + strand, within BT4354 at 5738.050 kb on + strand, within BT4354 at 5738.172 kb on + strand, within BT4354 at 5738.173 kb on - strand, within BT4354 at 5738.351 kb on + strand, within BT4354 at 5738.416 kb on + strand, within BT4354 at 5738.416 kb on + strand, within BT4354 at 5738.417 kb on - strand, within BT4354 at 5738.418 kb on + strand, within BT4354 at 5738.419 kb on - strand, within BT4354 at 5738.423 kb on - strand, within BT4354 at 5738.439 kb on - strand, within BT4354 at 5738.439 kb on - strand, within BT4354 at 5738.439 kb on - strand, within BT4354 at 5738.482 kb on + strand, within BT4354 at 5738.510 kb on - strand, within BT4354 at 5738.561 kb on - strand, within BT4354 at 5738.601 kb on - strand, within BT4354 at 5738.710 kb on - strand, within BT4354 at 5738.710 kb on - strand, within BT4354 at 5738.783 kb on - strand, within BT4354 at 5738.809 kb on + strand, within BT4354 at 5738.816 kb on + strand, within BT4354 at 5738.817 kb on - strand, within BT4354 at 5738.870 kb on - strand, within BT4354 at 5739.009 kb on - strand at 5739.116 kb on - strand at 5739.149 kb on + strand at 5739.150 kb on - strand at 5739.157 kb on + strand at 5739.158 kb on - strand at 5739.176 kb on + strand at 5739.177 kb on - strand at 5739.177 kb on - strand at 5739.185 kb on + strand at 5739.191 kb on + strand at 5739.191 kb on + strand at 5739.192 kb on - strand at 5739.195 kb on + strand at 5739.195 kb on + strand at 5739.226 kb on - strand at 5739.228 kb on + strand at 5739.385 kb on + strand at 5739.449 kb on + strand at 5739.479 kb on - strand at 5739.492 kb on + strand at 5739.493 kb on - strand at 5739.493 kb on - strand at 5739.494 kb on + strand at 5739.494 kb on + strand at 5739.494 kb on + strand at 5739.494 kb on + strand at 5739.495 kb on - strand at 5739.504 kb on - strand at 5739.537 kb on + strand at 5739.538 kb on - strand at 5739.538 kb on - strand at 5739.538 kb on - strand at 5739.542 kb on + strand at 5739.575 kb on + strand at 5739.576 kb on - strand at 5739.595 kb on + strand at 5739.671 kb on + strand, within BT4355 at 5739.672 kb on - strand, within BT4355 at 5739.790 kb on + strand, within BT4355 at 5739.791 kb on - strand, within BT4355 at 5739.791 kb on - strand, within BT4355 at 5739.791 kb on - strand, within BT4355 at 5739.791 kb on - strand, within BT4355 at 5739.812 kb on + strand, within BT4355 at 5739.862 kb on + strand, within BT4355 at 5739.947 kb on - strand, within BT4355 at 5740.004 kb on + strand, within BT4355 at 5740.013 kb on - strand, within BT4355 at 5740.013 kb on - strand, within BT4355 at 5740.022 kb on + strand, within BT4355 at 5740.026 kb on - strand, within BT4355 at 5740.026 kb on - strand, within BT4355 at 5740.055 kb on - strand, within BT4355 at 5740.100 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Paraquat dichloride 0.1 mg/ml remove 5,737,149 - -0.6 5,737,152 + -0.9 5,737,162 + -2.4 5,737,220 + -1.4 5,737,276 - +1.1 5,737,409 + -2.3 5,737,415 + -2.1 5,737,446 + BT4354 0.10 -1.1 5,737,633 - BT4354 0.20 -2.7 5,737,766 + BT4354 0.27 -1.6 5,737,793 + BT4354 0.28 -1.9 5,737,794 - BT4354 0.28 -1.3 5,737,795 + BT4354 0.28 -2.3 5,738,032 + BT4354 0.41 -1.6 5,738,050 + BT4354 0.42 +0.3 5,738,172 + BT4354 0.48 -0.5 5,738,173 - BT4354 0.48 -0.4 5,738,351 + BT4354 0.58 -2.3 5,738,416 + BT4354 0.61 -0.2 5,738,416 + BT4354 0.61 -3.6 5,738,417 - BT4354 0.61 -0.2 5,738,418 + BT4354 0.61 -2.3 5,738,419 - BT4354 0.61 -1.8 5,738,423 - BT4354 0.62 -0.5 5,738,439 - BT4354 0.62 -0.9 5,738,439 - BT4354 0.62 -1.6 5,738,439 - BT4354 0.62 -2.6 5,738,482 + BT4354 0.65 -4.7 5,738,510 - BT4354 0.66 -0.5 5,738,561 - BT4354 0.69 -2.3 5,738,601 - BT4354 0.71 -0.5 5,738,710 - BT4354 0.77 -2.8 5,738,710 - BT4354 0.77 -3.1 5,738,783 - BT4354 0.81 +0.1 5,738,809 + BT4354 0.82 -0.6 5,738,816 + BT4354 0.82 -3.1 5,738,817 - BT4354 0.82 -2.1 5,738,870 - BT4354 0.85 -0.6 5,739,009 - +1.0 5,739,116 - +2.1 5,739,149 + -0.7 5,739,150 - -1.5 5,739,157 + +1.0 5,739,158 - -0.5 5,739,176 + +0.6 5,739,177 - -1.5 5,739,177 - -3.7 5,739,185 + -1.5 5,739,191 + -0.7 5,739,191 + +0.5 5,739,192 - -1.5 5,739,195 + -1.1 5,739,195 + -2.9 5,739,226 - -1.9 5,739,228 + -3.7 5,739,385 + -0.9 5,739,449 + -3.7 5,739,479 - -3.6 5,739,492 + -1.7 5,739,493 - -0.6 5,739,493 - -1.5 5,739,494 + -1.1 5,739,494 + -1.5 5,739,494 + -2.6 5,739,494 + -1.1 5,739,495 - -1.8 5,739,504 - -1.4 5,739,537 + -1.3 5,739,538 - -2.3 5,739,538 - +0.5 5,739,538 - -1.6 5,739,542 + -0.6 5,739,575 + -0.0 5,739,576 - +1.5 5,739,595 + -1.8 5,739,671 + BT4355 0.23 -0.5 5,739,672 - BT4355 0.23 -0.5 5,739,790 + BT4355 0.43 -0.7 5,739,791 - BT4355 0.43 -1.5 5,739,791 - BT4355 0.43 -3.3 5,739,791 - BT4355 0.43 -3.1 5,739,791 - BT4355 0.43 -0.6 5,739,812 + BT4355 0.47 +1.4 5,739,862 + BT4355 0.56 -1.0 5,739,947 - BT4355 0.70 +0.5 5,740,004 + BT4355 0.80 -2.9 5,740,013 - BT4355 0.82 -3.2 5,740,013 - BT4355 0.82 -1.4 5,740,022 + BT4355 0.84 +1.6 5,740,026 - BT4355 0.84 +0.5 5,740,026 - BT4355 0.84 +0.3 5,740,055 - BT4355 0.89 +0.1 5,740,100 - -2.6
Or see this region's nucleotide sequence