Experiment: BHIS with Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4214 and BT4215 are separated by 152 nucleotides BT4215 and BT4216 are separated by 38 nucleotides BT4216 and BT4217 are separated by 2 nucleotides BT4217 and BT4218 are separated by 46 nucleotides
BT4214: BT4214 - conserved hypothetical protein (NCBI ptt file), at 5,551,068 to 5,551,940
BT4214
BT4215: BT4215 - shikimate dehydrogenase (EC 1.1.1.25) (from data) , at 5,552,093 to 5,552,839
BT4215
BT4216: BT4216 - ubiquinone/menaquinone biosynthesis methyltransferase (NCBI ptt file), at 5,552,878 to 5,553,615
BT4216
BT4217: BT4217 - phosphoribosylamidoimidazole-succinocarboxamide synthase (NCBI ptt file), at 5,553,618 to 5,554,562
BT4217
BT4218: BT4218 - phosphate starvation-inducible protein, PhoH (NCBI ptt file), at 5,554,609 to 5,555,625
BT4218
Position (kb)
5552
5553
5554 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5551.905 kb on + strand at 5551.975 kb on - strand at 5551.988 kb on + strand at 5551.989 kb on - strand at 5551.991 kb on + strand at 5551.991 kb on + strand at 5551.992 kb on - strand at 5551.992 kb on - strand at 5552.021 kb on + strand at 5552.021 kb on + strand at 5552.022 kb on - strand at 5552.036 kb on - strand at 5552.057 kb on + strand at 5552.089 kb on + strand at 5552.090 kb on - strand at 5552.863 kb on - strand at 5552.863 kb on - strand at 5553.595 kb on - strand at 5553.620 kb on - strand at 5553.932 kb on - strand, within BT4217 at 5553.936 kb on - strand, within BT4217
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Paraquat dichloride 0.1 mg/ml remove 5,551,905 + +0.7 5,551,975 - -2.4 5,551,988 + +1.8 5,551,989 - +0.5 5,551,991 + +0.4 5,551,991 + -0.4 5,551,992 - +0.4 5,551,992 - -0.6 5,552,021 + +0.6 5,552,021 + -1.7 5,552,022 - +1.2 5,552,036 - +0.7 5,552,057 + +0.6 5,552,089 + -1.9 5,552,090 - -3.1 5,552,863 - +0.9 5,552,863 - +1.1 5,553,595 - -3.4 5,553,620 - +0.6 5,553,932 - BT4217 0.33 +1.4 5,553,936 - BT4217 0.34 -0.1
Or see this region's nucleotide sequence