Experiment: BHIS with Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2971 and BT2972 are separated by 425 nucleotides BT2972 and BT2973 are separated by 74 nucleotides BT2973 and BT2974 are separated by 14 nucleotides BT2974 and BT2975 overlap by 17 nucleotides
BT2971: BT2971 - two-component system sensor histidine kinase/response regulator, hybrid ('one-component system') (NCBI ptt file), at 3,747,864 to 3,751,904
BT2971
BT2972: BT2972 - conserved hypothetical protein (NCBI ptt file), at 3,752,330 to 3,753,103
BT2972
BT2973: BT2973 - transcriptional regulator (NCBI ptt file), at 3,753,178 to 3,753,528
BT2973
BT2974: BT2974 - hypothetical protein (NCBI ptt file), at 3,753,543 to 3,753,761
BT2974
BT2975: BT2975 - putative nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (NCBI ptt file), at 3,753,745 to 3,754,449
BT2975
Position (kb)
3752
3753
3754 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3751.395 kb on + strand, within BT2971 at 3751.395 kb on + strand, within BT2971 at 3751.396 kb on - strand, within BT2971 at 3751.396 kb on - strand, within BT2971 at 3751.396 kb on - strand, within BT2971 at 3751.414 kb on + strand, within BT2971 at 3751.414 kb on + strand, within BT2971 at 3751.414 kb on + strand, within BT2971 at 3751.414 kb on + strand, within BT2971 at 3751.415 kb on - strand, within BT2971 at 3751.415 kb on - strand, within BT2971 at 3751.470 kb on - strand, within BT2971 at 3751.487 kb on - strand, within BT2971 at 3751.489 kb on + strand, within BT2971 at 3751.489 kb on + strand, within BT2971 at 3751.520 kb on + strand at 3751.521 kb on - strand at 3751.543 kb on + strand at 3751.587 kb on - strand at 3751.622 kb on - strand at 3751.637 kb on + strand at 3751.647 kb on + strand at 3751.664 kb on + strand at 3751.665 kb on - strand at 3751.667 kb on + strand at 3751.667 kb on + strand at 3751.667 kb on + strand at 3751.707 kb on + strand at 3751.707 kb on + strand at 3751.728 kb on - strand at 3751.728 kb on - strand at 3751.728 kb on - strand at 3751.742 kb on + strand at 3751.742 kb on + strand at 3751.777 kb on + strand at 3751.816 kb on - strand at 3751.842 kb on - strand at 3751.866 kb on + strand at 3751.867 kb on - strand at 3751.913 kb on + strand at 3751.913 kb on + strand at 3751.917 kb on + strand at 3751.980 kb on + strand at 3752.112 kb on + strand at 3752.113 kb on - strand at 3752.133 kb on + strand at 3752.159 kb on - strand at 3752.263 kb on + strand at 3752.279 kb on + strand at 3752.280 kb on - strand at 3752.291 kb on + strand at 3752.322 kb on + strand at 3752.344 kb on + strand at 3752.344 kb on + strand at 3752.344 kb on + strand at 3752.350 kb on + strand at 3752.351 kb on - strand at 3752.351 kb on - strand at 3752.380 kb on + strand at 3752.381 kb on - strand at 3752.384 kb on + strand at 3752.554 kb on - strand, within BT2972 at 3752.573 kb on - strand, within BT2972 at 3752.708 kb on - strand, within BT2972 at 3752.710 kb on - strand, within BT2972 at 3752.837 kb on + strand, within BT2972 at 3752.866 kb on + strand, within BT2972 at 3752.867 kb on - strand, within BT2972 at 3752.867 kb on - strand, within BT2972 at 3752.894 kb on - strand, within BT2972 at 3752.964 kb on + strand, within BT2972 at 3752.965 kb on - strand, within BT2972 at 3753.032 kb on + strand at 3753.032 kb on + strand at 3753.047 kb on + strand at 3753.048 kb on - strand at 3753.052 kb on + strand at 3753.065 kb on + strand at 3753.066 kb on - strand at 3753.075 kb on - strand at 3753.075 kb on - strand at 3753.079 kb on - strand at 3753.083 kb on + strand at 3753.084 kb on - strand at 3753.084 kb on - strand at 3753.087 kb on + strand at 3753.088 kb on - strand at 3753.109 kb on - strand at 3753.154 kb on + strand at 3753.157 kb on + strand at 3753.211 kb on - strand at 3753.254 kb on + strand, within BT2973 at 3753.255 kb on - strand, within BT2973 at 3753.358 kb on + strand, within BT2973 at 3753.358 kb on + strand, within BT2973 at 3753.481 kb on - strand, within BT2973 at 3753.526 kb on + strand at 3753.673 kb on + strand, within BT2974 at 3753.674 kb on - strand, within BT2974 at 3753.677 kb on - strand, within BT2974 at 3753.695 kb on + strand, within BT2974 at 3753.731 kb on + strand, within BT2974 at 3753.738 kb on - strand, within BT2974 at 3753.759 kb on + strand at 3753.828 kb on + strand, within BT2975 at 3753.828 kb on + strand, within BT2975 at 3753.829 kb on - strand, within BT2975 at 3753.829 kb on - strand, within BT2975 at 3753.829 kb on - strand, within BT2975 at 3753.829 kb on - strand, within BT2975 at 3753.834 kb on + strand, within BT2975 at 3753.835 kb on - strand, within BT2975 at 3753.853 kb on + strand, within BT2975 at 3753.861 kb on - strand, within BT2975 at 3753.885 kb on - strand, within BT2975 at 3753.910 kb on + strand, within BT2975 at 3753.911 kb on - strand, within BT2975 at 3753.911 kb on - strand, within BT2975 at 3753.911 kb on - strand, within BT2975 at 3753.940 kb on + strand, within BT2975 at 3753.940 kb on + strand, within BT2975 at 3753.955 kb on + strand, within BT2975 at 3754.008 kb on + strand, within BT2975 at 3754.042 kb on - strand, within BT2975 at 3754.061 kb on + strand, within BT2975 at 3754.062 kb on - strand, within BT2975 at 3754.062 kb on - strand, within BT2975 at 3754.084 kb on + strand, within BT2975 at 3754.085 kb on - strand, within BT2975 at 3754.086 kb on + strand, within BT2975 at 3754.087 kb on - strand, within BT2975 at 3754.088 kb on + strand, within BT2975 at 3754.089 kb on - strand, within BT2975 at 3754.089 kb on - strand, within BT2975
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Paraquat dichloride 0.1 mg/ml remove 3,751,395 + BT2971 0.87 -0.4 3,751,395 + BT2971 0.87 +0.6 3,751,396 - BT2971 0.87 +0.8 3,751,396 - BT2971 0.87 -2.8 3,751,396 - BT2971 0.87 -0.7 3,751,414 + BT2971 0.88 -1.3 3,751,414 + BT2971 0.88 +1.3 3,751,414 + BT2971 0.88 -0.4 3,751,414 + BT2971 0.88 +0.3 3,751,415 - BT2971 0.88 +1.0 3,751,415 - BT2971 0.88 +0.8 3,751,470 - BT2971 0.89 +1.6 3,751,487 - BT2971 0.90 -0.2 3,751,489 + BT2971 0.90 +0.3 3,751,489 + BT2971 0.90 -0.7 3,751,520 + -1.5 3,751,521 - -2.7 3,751,543 + +0.5 3,751,587 - +0.1 3,751,622 - -0.1 3,751,637 + -0.9 3,751,647 + +0.1 3,751,664 + -1.0 3,751,665 - -1.2 3,751,667 + +0.1 3,751,667 + -1.6 3,751,667 + +0.3 3,751,707 + -1.2 3,751,707 + -2.4 3,751,728 - +1.3 3,751,728 - +0.6 3,751,728 - -1.6 3,751,742 + -2.0 3,751,742 + +0.5 3,751,777 + +1.1 3,751,816 - -3.1 3,751,842 - -1.1 3,751,866 + -2.0 3,751,867 - -0.3 3,751,913 + -0.1 3,751,913 + -1.5 3,751,917 + -1.5 3,751,980 + -0.2 3,752,112 + -0.6 3,752,113 - -0.9 3,752,133 + -0.9 3,752,159 - -1.1 3,752,263 + +2.3 3,752,279 + -2.3 3,752,280 - +1.3 3,752,291 + +2.9 3,752,322 + -1.5 3,752,344 + +1.2 3,752,344 + -0.6 3,752,344 + -2.6 3,752,350 + +1.3 3,752,351 - -0.7 3,752,351 - +1.1 3,752,380 + +0.1 3,752,381 - -3.5 3,752,384 + +2.1 3,752,554 - BT2972 0.29 -3.1 3,752,573 - BT2972 0.31 +1.8 3,752,708 - BT2972 0.49 -2.4 3,752,710 - BT2972 0.49 -2.5 3,752,837 + BT2972 0.66 -2.0 3,752,866 + BT2972 0.69 -2.0 3,752,867 - BT2972 0.69 -0.7 3,752,867 - BT2972 0.69 -2.6 3,752,894 - BT2972 0.73 -2.6 3,752,964 + BT2972 0.82 +1.3 3,752,965 - BT2972 0.82 -3.2 3,753,032 + -0.9 3,753,032 + -0.2 3,753,047 + -1.0 3,753,048 - -0.5 3,753,052 + +1.9 3,753,065 + -2.1 3,753,066 - -2.3 3,753,075 - -0.5 3,753,075 - -2.5 3,753,079 - +0.6 3,753,083 + -1.1 3,753,084 - -1.8 3,753,084 - +2.3 3,753,087 + -0.5 3,753,088 - -1.0 3,753,109 - -1.4 3,753,154 + +1.3 3,753,157 + +0.2 3,753,211 - -2.7 3,753,254 + BT2973 0.22 -0.2 3,753,255 - BT2973 0.22 +0.1 3,753,358 + BT2973 0.51 -3.6 3,753,358 + BT2973 0.51 -0.9 3,753,481 - BT2973 0.86 -0.2 3,753,526 + +1.3 3,753,673 + BT2974 0.59 +0.3 3,753,674 - BT2974 0.60 +1.6 3,753,677 - BT2974 0.61 -0.0 3,753,695 + BT2974 0.69 -3.0 3,753,731 + BT2974 0.86 -1.5 3,753,738 - BT2974 0.89 -1.3 3,753,759 + -0.9 3,753,828 + BT2975 0.12 +1.3 3,753,828 + BT2975 0.12 +1.3 3,753,829 - BT2975 0.12 -3.2 3,753,829 - BT2975 0.12 -2.3 3,753,829 - BT2975 0.12 -0.2 3,753,829 - BT2975 0.12 -1.8 3,753,834 + BT2975 0.13 -2.5 3,753,835 - BT2975 0.13 -1.9 3,753,853 + BT2975 0.15 -1.7 3,753,861 - BT2975 0.16 -0.2 3,753,885 - BT2975 0.20 -3.6 3,753,910 + BT2975 0.23 -1.0 3,753,911 - BT2975 0.24 +0.0 3,753,911 - BT2975 0.24 +1.3 3,753,911 - BT2975 0.24 +0.2 3,753,940 + BT2975 0.28 -1.8 3,753,940 + BT2975 0.28 -2.8 3,753,955 + BT2975 0.30 -1.6 3,754,008 + BT2975 0.37 -0.9 3,754,042 - BT2975 0.42 -0.7 3,754,061 + BT2975 0.45 +0.3 3,754,062 - BT2975 0.45 -1.8 3,754,062 - BT2975 0.45 -1.3 3,754,084 + BT2975 0.48 -1.0 3,754,085 - BT2975 0.48 +1.3 3,754,086 + BT2975 0.48 -0.7 3,754,087 - BT2975 0.49 +1.3 3,754,088 + BT2975 0.49 -0.2 3,754,089 - BT2975 0.49 +0.9 3,754,089 - BT2975 0.49 +0.3
Or see this region's nucleotide sequence