Experiment: BHIS with Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2467 and BT2468 are separated by 481 nucleotides BT2468 and BT2469 are separated by 158 nucleotides BT2469 and BT2470 are separated by 521 nucleotides
BT2467: BT2467 - hypothetical protein (NCBI ptt file), at 3,088,513 to 3,088,923
BT2467
BT2468: BT2468 - conserved hypothetical protein (NCBI ptt file), at 3,089,405 to 3,089,698
BT2468
BT2469: BT2469 - integrase (NCBI ptt file), at 3,089,857 to 3,090,117
BT2469
BT2470: BT2470 - hypothetical protein (NCBI ptt file), at 3,090,639 to 3,090,848
BT2470
Position (kb)
3089
3090 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3088.501 kb on + strand at 3088.501 kb on + strand at 3088.502 kb on - strand at 3088.513 kb on - strand at 3088.546 kb on + strand at 3088.547 kb on - strand at 3088.547 kb on - strand at 3088.551 kb on + strand at 3088.568 kb on + strand, within BT2467 at 3088.577 kb on + strand, within BT2467 at 3088.578 kb on - strand, within BT2467 at 3088.578 kb on - strand, within BT2467 at 3088.598 kb on - strand, within BT2467 at 3088.598 kb on - strand, within BT2467 at 3088.669 kb on - strand, within BT2467 at 3088.702 kb on + strand, within BT2467 at 3088.772 kb on - strand, within BT2467 at 3088.779 kb on + strand, within BT2467 at 3088.835 kb on + strand, within BT2467 at 3088.944 kb on - strand at 3089.128 kb on + strand at 3089.128 kb on + strand at 3089.237 kb on - strand at 3089.301 kb on - strand at 3089.333 kb on + strand at 3089.391 kb on + strand at 3089.392 kb on - strand at 3089.416 kb on + strand at 3089.559 kb on + strand, within BT2468 at 3089.606 kb on - strand, within BT2468 at 3089.615 kb on - strand, within BT2468 at 3089.640 kb on - strand, within BT2468 at 3089.640 kb on - strand, within BT2468 at 3089.641 kb on + strand, within BT2468 at 3089.641 kb on + strand, within BT2468 at 3089.675 kb on - strand at 3089.675 kb on - strand at 3089.696 kb on + strand at 3089.696 kb on + strand at 3089.793 kb on + strand at 3089.858 kb on + strand at 3089.859 kb on - strand at 3089.859 kb on - strand at 3089.859 kb on - strand at 3089.864 kb on + strand at 3089.864 kb on + strand at 3089.865 kb on - strand at 3089.874 kb on + strand at 3090.003 kb on + strand at 3090.017 kb on + strand, within BT2469 at 3090.017 kb on + strand, within BT2469 at 3090.019 kb on + strand, within BT2469 at 3090.019 kb on + strand, within BT2469 at 3090.020 kb on - strand, within BT2469 at 3090.020 kb on - strand, within BT2469 at 3090.028 kb on - strand, within BT2469 at 3090.069 kb on + strand, within BT2469 at 3090.077 kb on + strand, within BT2469 at 3090.089 kb on + strand, within BT2469 at 3090.089 kb on + strand, within BT2469 at 3090.090 kb on - strand, within BT2469 at 3090.100 kb on - strand at 3090.136 kb on + strand at 3090.175 kb on + strand at 3090.176 kb on - strand at 3090.177 kb on + strand at 3090.183 kb on + strand at 3090.183 kb on + strand at 3090.183 kb on + strand at 3090.183 kb on + strand at 3090.184 kb on - strand at 3090.185 kb on + strand at 3090.186 kb on - strand at 3090.186 kb on - strand at 3090.187 kb on + strand at 3090.188 kb on - strand at 3090.191 kb on + strand at 3090.192 kb on - strand at 3090.193 kb on + strand at 3090.194 kb on - strand at 3090.194 kb on - strand at 3090.199 kb on + strand at 3090.200 kb on - strand at 3090.202 kb on - strand at 3090.307 kb on - strand at 3090.308 kb on + strand at 3090.309 kb on - strand at 3090.314 kb on + strand at 3090.314 kb on + strand at 3090.316 kb on + strand at 3090.316 kb on + strand at 3090.316 kb on + strand at 3090.450 kb on - strand at 3090.450 kb on - strand at 3090.455 kb on + strand at 3090.456 kb on - strand at 3090.456 kb on - strand at 3090.458 kb on - strand at 3090.458 kb on - strand at 3090.475 kb on + strand at 3090.476 kb on - strand at 3090.500 kb on - strand at 3090.590 kb on - strand at 3090.590 kb on - strand at 3090.592 kb on - strand at 3090.592 kb on - strand at 3090.619 kb on + strand at 3090.627 kb on + strand at 3090.642 kb on + strand at 3090.642 kb on + strand at 3090.642 kb on + strand at 3090.643 kb on - strand at 3090.698 kb on - strand, within BT2470
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Paraquat dichloride 0.1 mg/ml remove 3,088,501 + +0.6 3,088,501 + -1.9 3,088,502 - +0.2 3,088,513 - -1.7 3,088,546 + +2.5 3,088,547 - -1.4 3,088,547 - -1.5 3,088,551 + +1.0 3,088,568 + BT2467 0.13 -0.6 3,088,577 + BT2467 0.16 -0.3 3,088,578 - BT2467 0.16 -0.5 3,088,578 - BT2467 0.16 +0.5 3,088,598 - BT2467 0.21 -1.0 3,088,598 - BT2467 0.21 -2.0 3,088,669 - BT2467 0.38 -1.6 3,088,702 + BT2467 0.46 -1.0 3,088,772 - BT2467 0.63 +0.1 3,088,779 + BT2467 0.65 -0.0 3,088,835 + BT2467 0.78 -1.9 3,088,944 - -1.4 3,089,128 + -2.4 3,089,128 + +2.3 3,089,237 - -2.2 3,089,301 - +0.8 3,089,333 + -1.6 3,089,391 + -2.6 3,089,392 - +0.2 3,089,416 + -1.6 3,089,559 + BT2468 0.52 -0.6 3,089,606 - BT2468 0.68 -3.1 3,089,615 - BT2468 0.71 -1.5 3,089,640 - BT2468 0.80 -1.5 3,089,640 - BT2468 0.80 +0.1 3,089,641 + BT2468 0.80 -1.4 3,089,641 + BT2468 0.80 -0.5 3,089,675 - -0.9 3,089,675 - -0.5 3,089,696 + -1.8 3,089,696 + -0.6 3,089,793 + -0.9 3,089,858 + +1.7 3,089,859 - -2.9 3,089,859 - -0.6 3,089,859 - -2.7 3,089,864 + -3.7 3,089,864 + -0.9 3,089,865 - -0.3 3,089,874 + +1.5 3,090,003 + -0.0 3,090,017 + BT2469 0.61 +0.5 3,090,017 + BT2469 0.61 -0.0 3,090,019 + BT2469 0.62 -3.0 3,090,019 + BT2469 0.62 +0.5 3,090,020 - BT2469 0.62 -1.6 3,090,020 - BT2469 0.62 -3.0 3,090,028 - BT2469 0.66 -2.9 3,090,069 + BT2469 0.81 -2.9 3,090,077 + BT2469 0.84 -1.0 3,090,089 + BT2469 0.89 -0.0 3,090,089 + BT2469 0.89 +0.2 3,090,090 - BT2469 0.89 -1.6 3,090,100 - +0.2 3,090,136 + -1.2 3,090,175 + -3.5 3,090,176 - -1.9 3,090,177 + -1.3 3,090,183 + +0.2 3,090,183 + +0.3 3,090,183 + -1.1 3,090,183 + -3.3 3,090,184 - +0.8 3,090,185 + -3.2 3,090,186 - +0.2 3,090,186 - +0.5 3,090,187 + +0.6 3,090,188 - -2.5 3,090,191 + +0.4 3,090,192 - +0.1 3,090,193 + -1.6 3,090,194 - -0.0 3,090,194 - -2.4 3,090,199 + +1.0 3,090,200 - +0.5 3,090,202 - +0.6 3,090,307 - -1.4 3,090,308 + -0.9 3,090,309 - +2.5 3,090,314 + -0.9 3,090,314 + +0.2 3,090,316 + -2.9 3,090,316 + -0.5 3,090,316 + -1.2 3,090,450 - -2.0 3,090,450 - -0.1 3,090,455 + +1.5 3,090,456 - -0.4 3,090,456 - -1.4 3,090,458 - -2.1 3,090,458 - -1.2 3,090,475 + +1.9 3,090,476 - -0.9 3,090,500 - -1.3 3,090,590 - -1.7 3,090,590 - +0.9 3,090,592 - -0.9 3,090,592 - -0.0 3,090,619 + -1.1 3,090,627 + -0.4 3,090,642 + +0.2 3,090,642 + +0.8 3,090,642 + +0.9 3,090,643 - -4.6 3,090,698 - BT2470 0.28 -2.2
Or see this region's nucleotide sequence