Experiment: BHIS with Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tRNA-Ser and BT2056 are separated by 120 nucleotides BT2056 and BT2057 overlap by 7 nucleotides BT2057 and BT2058 are separated by 15 nucleotides
BT_t34: tRNA-Ser - tRNA-Ser (RefSeq), at 2,575,512 to 2,575,599
tRNA-Ser
BT2056: BT2056 - conserved hypothetical protein (NCBI ptt file), at 2,575,720 to 2,576,496
BT2056
BT2057: BT2057 - hypothetical protein (NCBI ptt file), at 2,576,490 to 2,577,206
BT2057
BT2058: BT2058 - putative isoprenyl synthetase (NCBI ptt file), at 2,577,222 to 2,578,196
BT2058
Position (kb)
2576
2577
2578 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2575.553 kb on - strand, within tRNA-Ser at 2575.681 kb on - strand at 2575.681 kb on - strand at 2575.699 kb on + strand at 2575.818 kb on + strand, within BT2056 at 2575.819 kb on - strand, within BT2056 at 2576.079 kb on + strand, within BT2056 at 2576.082 kb on - strand, within BT2056 at 2576.083 kb on + strand, within BT2056 at 2576.083 kb on + strand, within BT2056 at 2576.083 kb on + strand, within BT2056 at 2576.085 kb on + strand, within BT2056 at 2576.087 kb on + strand, within BT2056 at 2576.127 kb on + strand, within BT2056 at 2576.127 kb on + strand, within BT2056 at 2576.227 kb on + strand, within BT2056 at 2576.235 kb on + strand, within BT2056 at 2576.236 kb on - strand, within BT2056 at 2576.301 kb on + strand, within BT2056 at 2576.394 kb on + strand, within BT2056 at 2576.400 kb on + strand, within BT2056 at 2576.484 kb on + strand at 2576.484 kb on + strand at 2576.488 kb on + strand at 2576.489 kb on - strand at 2576.539 kb on + strand at 2576.543 kb on - strand at 2576.719 kb on + strand, within BT2057 at 2576.719 kb on + strand, within BT2057 at 2576.740 kb on + strand, within BT2057 at 2576.752 kb on - strand, within BT2057 at 2576.773 kb on + strand, within BT2057 at 2576.893 kb on + strand, within BT2057 at 2576.947 kb on - strand, within BT2057 at 2576.947 kb on - strand, within BT2057 at 2576.966 kb on + strand, within BT2057 at 2576.968 kb on + strand, within BT2057 at 2576.968 kb on + strand, within BT2057 at 2576.968 kb on + strand, within BT2057 at 2576.969 kb on - strand, within BT2057 at 2577.038 kb on + strand, within BT2057 at 2577.125 kb on + strand, within BT2057 at 2577.128 kb on - strand, within BT2057 at 2577.165 kb on + strand at 2577.200 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Paraquat dichloride 0.1 mg/ml remove 2,575,553 - tRNA-Ser BT_t34 0.47 -0.6 2,575,681 - -3.3 2,575,681 - +1.7 2,575,699 + +2.4 2,575,818 + BT2056 0.13 -2.5 2,575,819 - BT2056 0.13 +2.0 2,576,079 + BT2056 0.46 -0.9 2,576,082 - BT2056 0.47 -0.2 2,576,083 + BT2056 0.47 -1.5 2,576,083 + BT2056 0.47 +0.1 2,576,083 + BT2056 0.47 +0.4 2,576,085 + BT2056 0.47 +1.2 2,576,087 + BT2056 0.47 +1.4 2,576,127 + BT2056 0.52 +0.1 2,576,127 + BT2056 0.52 -0.1 2,576,227 + BT2056 0.65 -1.5 2,576,235 + BT2056 0.66 +0.6 2,576,236 - BT2056 0.66 -1.6 2,576,301 + BT2056 0.75 +0.1 2,576,394 + BT2056 0.87 -0.5 2,576,400 + BT2056 0.88 +0.4 2,576,484 + -1.2 2,576,484 + -0.3 2,576,488 + +0.6 2,576,489 - +0.1 2,576,539 + -1.5 2,576,543 - -2.4 2,576,719 + BT2057 0.32 +2.8 2,576,719 + BT2057 0.32 +1.5 2,576,740 + BT2057 0.35 -1.9 2,576,752 - BT2057 0.37 -0.1 2,576,773 + BT2057 0.39 +1.1 2,576,893 + BT2057 0.56 +0.4 2,576,947 - BT2057 0.64 -0.4 2,576,947 - BT2057 0.64 +0.3 2,576,966 + BT2057 0.66 +1.3 2,576,968 + BT2057 0.67 -1.9 2,576,968 + BT2057 0.67 -0.5 2,576,968 + BT2057 0.67 +1.3 2,576,969 - BT2057 0.67 +1.9 2,577,038 + BT2057 0.76 +0.2 2,577,125 + BT2057 0.89 +1.4 2,577,128 - BT2057 0.89 -1.3 2,577,165 + +1.6 2,577,200 - -0.3
Or see this region's nucleotide sequence