Experiment: BHIS with Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1675 and BT1676 overlap by 6 nucleotides BT1676 and BT1677 are separated by 30 nucleotides BT1677 and BT1678 overlap by 20 nucleotides
BT1675: BT1675 - conserved protein, with a conserved TPR domain (NCBI ptt file), at 2,067,782 to 2,069,986
BT1675
BT1676: BT1676 - Maf protein (NCBI ptt file), at 2,069,981 to 2,070,562
BT1676
BT1677: BT1677 - putative hydrolase (NCBI ptt file), at 2,070,593 to 2,071,114
BT1677
BT1678: BT1678 - conserved hypothetical protein (NCBI ptt file), at 2,071,095 to 2,071,889
BT1678
Position (kb)
2069
2070
2071 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2069.254 kb on + strand, within BT1675 at 2069.587 kb on + strand, within BT1675 at 2069.798 kb on + strand at 2070.030 kb on - strand at 2070.031 kb on + strand at 2070.034 kb on + strand at 2070.035 kb on - strand at 2070.035 kb on - strand at 2070.052 kb on + strand, within BT1676 at 2070.060 kb on - strand, within BT1676 at 2070.067 kb on + strand, within BT1676 at 2070.067 kb on + strand, within BT1676 at 2070.069 kb on + strand, within BT1676 at 2070.070 kb on - strand, within BT1676 at 2070.121 kb on - strand, within BT1676 at 2070.132 kb on + strand, within BT1676 at 2070.188 kb on - strand, within BT1676 at 2070.188 kb on - strand, within BT1676 at 2070.214 kb on + strand, within BT1676 at 2070.331 kb on + strand, within BT1676 at 2070.403 kb on - strand, within BT1676 at 2070.474 kb on + strand, within BT1676 at 2070.475 kb on - strand, within BT1676 at 2070.531 kb on - strand at 2070.531 kb on - strand at 2070.532 kb on + strand at 2070.533 kb on - strand at 2070.576 kb on - strand at 2070.587 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Paraquat dichloride 0.1 mg/ml remove 2,069,254 + BT1675 0.67 -1.0 2,069,587 + BT1675 0.82 -1.0 2,069,798 + -2.2 2,070,030 - -3.4 2,070,031 + +1.6 2,070,034 + +0.4 2,070,035 - +1.8 2,070,035 - -2.8 2,070,052 + BT1676 0.12 -1.2 2,070,060 - BT1676 0.14 -2.3 2,070,067 + BT1676 0.15 -3.2 2,070,067 + BT1676 0.15 -3.7 2,070,069 + BT1676 0.15 -0.8 2,070,070 - BT1676 0.15 -1.8 2,070,121 - BT1676 0.24 -0.4 2,070,132 + BT1676 0.26 -0.9 2,070,188 - BT1676 0.36 -1.6 2,070,188 - BT1676 0.36 -2.0 2,070,214 + BT1676 0.40 +0.6 2,070,331 + BT1676 0.60 -2.7 2,070,403 - BT1676 0.73 -1.9 2,070,474 + BT1676 0.85 -1.7 2,070,475 - BT1676 0.85 -1.2 2,070,531 - -2.3 2,070,531 - -1.4 2,070,532 + -1.0 2,070,533 - -1.6 2,070,576 - +0.4 2,070,587 + -0.4
Or see this region's nucleotide sequence