Experiment: BHIS with Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1539 and BT1540 are separated by 3 nucleotides BT1540 and BT1541 are separated by 88 nucleotides BT1541 and BT1542 are separated by 103 nucleotides
BT1539: BT1539 - cytidine deaminase (NCBI ptt file), at 1,899,104 to 1,899,499
BT1539
BT1540: BT1540 - transcriptional regulator (NCBI ptt file), at 1,899,503 to 1,900,342
BT1540
BT1541: BT1541 - conserved hypothetical protein (NCBI ptt file), at 1,900,431 to 1,901,012
BT1541
BT1542: BT1542 - putative pyridine nucleotide-disulfide oxidoreductase (NCBI ptt file), at 1,901,116 to 1,902,489
BT1542
Position (kb)
1900
1901
1902 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5
6 at 1899.431 kb on - strand, within BT1539 at 1899.435 kb on - strand, within BT1539 at 1899.596 kb on + strand, within BT1540 at 1899.604 kb on + strand, within BT1540 at 1899.657 kb on + strand, within BT1540 at 1899.657 kb on + strand, within BT1540 at 1899.658 kb on - strand, within BT1540 at 1899.729 kb on - strand, within BT1540 at 1900.037 kb on + strand, within BT1540 at 1900.047 kb on + strand, within BT1540 at 1900.086 kb on - strand, within BT1540 at 1900.141 kb on + strand, within BT1540 at 1900.216 kb on + strand, within BT1540 at 1900.308 kb on + strand at 1900.343 kb on + strand at 1900.496 kb on + strand, within BT1541 at 1900.513 kb on + strand, within BT1541 at 1900.704 kb on + strand, within BT1541 at 1900.723 kb on + strand, within BT1541 at 1900.793 kb on - strand, within BT1541 at 1900.827 kb on - strand, within BT1541 at 1900.827 kb on - strand, within BT1541 at 1901.062 kb on + strand at 1901.063 kb on - strand at 1901.063 kb on - strand at 1901.084 kb on - strand at 1901.125 kb on + strand at 1901.125 kb on + strand at 1901.125 kb on + strand at 1901.136 kb on + strand at 1901.139 kb on + strand at 1901.252 kb on + strand at 1901.260 kb on - strand, within BT1542 at 1901.308 kb on + strand, within BT1542 at 1901.326 kb on - strand, within BT1542 at 1901.334 kb on + strand, within BT1542 at 1901.335 kb on - strand, within BT1542 at 1901.341 kb on + strand, within BT1542 at 1901.342 kb on - strand, within BT1542 at 1901.342 kb on - strand, within BT1542 at 1901.434 kb on + strand, within BT1542 at 1901.435 kb on - strand, within BT1542 at 1901.443 kb on - strand, within BT1542 at 1901.556 kb on + strand, within BT1542 at 1901.556 kb on + strand, within BT1542 at 1901.558 kb on + strand, within BT1542 at 1901.558 kb on + strand, within BT1542 at 1901.559 kb on - strand, within BT1542 at 1901.588 kb on - strand, within BT1542 at 1901.595 kb on + strand, within BT1542 at 1901.638 kb on - strand, within BT1542 at 1901.681 kb on - strand, within BT1542 at 1901.884 kb on + strand, within BT1542 at 1901.884 kb on + strand, within BT1542 at 1902.009 kb on - strand, within BT1542 at 1902.012 kb on + strand, within BT1542
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Paraquat dichloride 0.1 mg/ml remove 1,899,431 - BT1539 0.83 -4.2 1,899,435 - BT1539 0.84 -1.2 1,899,596 + BT1540 0.11 -0.6 1,899,604 + BT1540 0.12 -1.2 1,899,657 + BT1540 0.18 +0.7 1,899,657 + BT1540 0.18 -2.4 1,899,658 - BT1540 0.18 +0.7 1,899,729 - BT1540 0.27 -1.4 1,900,037 + BT1540 0.64 -1.2 1,900,047 + BT1540 0.65 +1.0 1,900,086 - BT1540 0.69 -1.9 1,900,141 + BT1540 0.76 -0.6 1,900,216 + BT1540 0.85 +1.2 1,900,308 + +0.7 1,900,343 + -0.6 1,900,496 + BT1541 0.11 +0.0 1,900,513 + BT1541 0.14 -0.2 1,900,704 + BT1541 0.47 +6.5 1,900,723 + BT1541 0.50 -1.7 1,900,793 - BT1541 0.62 +0.3 1,900,827 - BT1541 0.68 +0.6 1,900,827 - BT1541 0.68 +1.0 1,901,062 + +0.8 1,901,063 - +0.9 1,901,063 - +1.3 1,901,084 - -1.9 1,901,125 + +0.3 1,901,125 + +0.5 1,901,125 + -2.6 1,901,136 + +2.6 1,901,139 + -2.7 1,901,252 + -0.2 1,901,260 - BT1542 0.10 -3.2 1,901,308 + BT1542 0.14 -2.6 1,901,326 - BT1542 0.15 -1.9 1,901,334 + BT1542 0.16 -1.2 1,901,335 - BT1542 0.16 -4.6 1,901,341 + BT1542 0.16 -0.6 1,901,342 - BT1542 0.16 +1.3 1,901,342 - BT1542 0.16 -2.6 1,901,434 + BT1542 0.23 +0.4 1,901,435 - BT1542 0.23 -0.3 1,901,443 - BT1542 0.24 -0.8 1,901,556 + BT1542 0.32 +0.7 1,901,556 + BT1542 0.32 -1.5 1,901,558 + BT1542 0.32 -3.2 1,901,558 + BT1542 0.32 -2.0 1,901,559 - BT1542 0.32 -5.7 1,901,588 - BT1542 0.34 -2.4 1,901,595 + BT1542 0.35 +0.4 1,901,638 - BT1542 0.38 -0.9 1,901,681 - BT1542 0.41 -2.8 1,901,884 + BT1542 0.56 -2.2 1,901,884 + BT1542 0.56 -4.5 1,902,009 - BT1542 0.65 +1.4 1,902,012 + BT1542 0.65 -2.0
Or see this region's nucleotide sequence