Experiment: BHIS with Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0203 and BT0204 are separated by 90 nucleotides BT0204 and BT0205 are separated by 114 nucleotides
BT0203: BT0203 - histidine biosynthesis bifunctional protein hisB (NCBI ptt file), at 218,282 to 219,406
BT0203
BT0204: BT0204 - conserved hypothetical protein (NCBI ptt file), at 219,497 to 220,006
BT0204
BT0205: BT0205 - glutamine-dependent NAD+ synthetase (NCBI ptt file), at 220,121 to 222,046
BT0205
Position (kb) 219
220
221 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 218.627 kb on - strand, within BT0203 at 218.627 kb on - strand, within BT0203 at 218.870 kb on - strand, within BT0203 at 218.873 kb on + strand, within BT0203 at 218.874 kb on - strand, within BT0203 at 218.874 kb on - strand, within BT0203 at 219.058 kb on - strand, within BT0203 at 219.116 kb on - strand, within BT0203 at 219.172 kb on - strand, within BT0203 at 219.310 kb on - strand at 219.365 kb on - strand at 219.419 kb on + strand at 219.420 kb on - strand at 219.557 kb on - strand, within BT0204 at 219.580 kb on - strand, within BT0204 at 219.592 kb on - strand, within BT0204 at 219.641 kb on + strand, within BT0204 at 219.642 kb on - strand, within BT0204 at 219.712 kb on + strand, within BT0204 at 219.846 kb on + strand, within BT0204 at 219.853 kb on + strand, within BT0204 at 219.854 kb on - strand, within BT0204 at 219.857 kb on + strand, within BT0204 at 219.863 kb on - strand, within BT0204 at 219.863 kb on - strand, within BT0204 at 219.935 kb on - strand, within BT0204 at 219.946 kb on + strand, within BT0204 at 219.947 kb on - strand, within BT0204 at 219.997 kb on - strand at 220.020 kb on - strand at 220.038 kb on - strand at 220.080 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Paraquat dichloride 0.1 mg/ml remove 218,627 - BT0203 0.31 -2.3 218,627 - BT0203 0.31 +0.7 218,870 - BT0203 0.52 -0.6 218,873 + BT0203 0.53 -4.1 218,874 - BT0203 0.53 +0.9 218,874 - BT0203 0.53 +0.4 219,058 - BT0203 0.69 -2.3 219,116 - BT0203 0.74 -0.7 219,172 - BT0203 0.79 -0.8 219,310 - -1.5 219,365 - -0.4 219,419 + -2.4 219,420 - -2.3 219,557 - BT0204 0.12 -2.9 219,580 - BT0204 0.16 +0.7 219,592 - BT0204 0.19 -1.0 219,641 + BT0204 0.28 -2.5 219,642 - BT0204 0.28 -0.4 219,712 + BT0204 0.42 -1.7 219,846 + BT0204 0.68 -3.0 219,853 + BT0204 0.70 -3.9 219,854 - BT0204 0.70 +1.3 219,857 + BT0204 0.71 -3.4 219,863 - BT0204 0.72 +1.0 219,863 - BT0204 0.72 -1.2 219,935 - BT0204 0.86 -1.0 219,946 + BT0204 0.88 -5.4 219,947 - BT0204 0.88 +0.3 219,997 - +1.1 220,020 - -2.7 220,038 - +0.5 220,080 - -2.9
Or see this region's nucleotide sequence