Experiment: BHIS with Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0003 and BT0004 are separated by 134 nucleotides BT0004 and BT0005 are separated by 8 nucleotides BT0005 and BT0006 are separated by 91 nucleotides BT0006 and BT0007 overlap by 78 nucleotides BT0007 and BT0008 are separated by 108 nucleotides
BT0003: BT0003 - hypothetical protein (NCBI ptt file), at 1,872 to 2,648
BT0003
BT0004: BT0004 - hypothetical protein (NCBI ptt file), at 2,783 to 3,451
BT0004
BT0005: BT0005 - 30S ribosomal protein S17 (NCBI ptt file), at 3,460 to 3,996
BT0005
BT0006: BT0006 - hypothetical protein (NCBI ptt file), at 4,088 to 4,534
BT0006
BT0007: BT0007 - hypothetical protein (NCBI ptt file), at 4,457 to 4,849
BT0007
BT0008: BT0008 - conserved hypothetical protein (NCBI ptt file), at 4,958 to 5,503
BT0008
Position (kb)
3
4 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2.483 kb on - strand, within BT0003 at 2.521 kb on - strand, within BT0003 at 2.528 kb on + strand, within BT0003 at 2.534 kb on + strand, within BT0003 at 2.535 kb on - strand, within BT0003 at 2.535 kb on - strand, within BT0003 at 2.556 kb on - strand, within BT0003 at 2.573 kb on + strand at 2.697 kb on - strand at 2.697 kb on - strand at 2.697 kb on - strand at 2.707 kb on - strand at 2.777 kb on - strand at 2.797 kb on - strand at 2.813 kb on - strand at 2.955 kb on - strand, within BT0004 at 2.955 kb on - strand, within BT0004 at 3.192 kb on + strand, within BT0004 at 3.232 kb on + strand, within BT0004 at 3.422 kb on - strand at 3.426 kb on - strand at 3.458 kb on - strand at 3.550 kb on - strand, within BT0005 at 3.557 kb on + strand, within BT0005 at 3.638 kb on - strand, within BT0005 at 3.694 kb on - strand, within BT0005 at 3.696 kb on + strand, within BT0005 at 3.697 kb on - strand, within BT0005 at 3.746 kb on + strand, within BT0005 at 3.747 kb on - strand, within BT0005 at 3.747 kb on - strand, within BT0005 at 3.785 kb on + strand, within BT0005 at 3.872 kb on - strand, within BT0005 at 3.917 kb on + strand, within BT0005 at 3.918 kb on - strand, within BT0005 at 3.918 kb on - strand, within BT0005 at 4.104 kb on + strand at 4.208 kb on - strand, within BT0006 at 4.258 kb on - strand, within BT0006 at 4.278 kb on - strand, within BT0006 at 4.278 kb on - strand, within BT0006 at 4.280 kb on - strand, within BT0006 at 4.280 kb on - strand, within BT0006 at 4.291 kb on - strand, within BT0006 at 4.301 kb on - strand, within BT0006 at 4.304 kb on + strand, within BT0006 at 4.324 kb on + strand, within BT0006 at 4.335 kb on - strand, within BT0006 at 4.364 kb on - strand, within BT0006 at 4.367 kb on + strand, within BT0006 at 4.486 kb on + strand at 4.567 kb on - strand, within BT0007 at 4.569 kb on - strand, within BT0007 at 4.571 kb on - strand, within BT0007 at 4.714 kb on - strand, within BT0007 at 4.714 kb on - strand, within BT0007 at 4.747 kb on + strand, within BT0007 at 4.969 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Paraquat dichloride 0.1 mg/ml remove 2,483 - BT0003 0.79 -2.5 2,521 - BT0003 0.84 +0.4 2,528 + BT0003 0.84 -1.2 2,534 + BT0003 0.85 +1.2 2,535 - BT0003 0.85 -0.4 2,535 - BT0003 0.85 -2.4 2,556 - BT0003 0.88 -2.2 2,573 + -1.3 2,697 - -3.5 2,697 - -3.6 2,697 - -0.2 2,707 - -0.0 2,777 - -1.9 2,797 - -1.3 2,813 - +0.2 2,955 - BT0004 0.26 +1.7 2,955 - BT0004 0.26 -1.3 3,192 + BT0004 0.61 -2.2 3,232 + BT0004 0.67 +0.4 3,422 - -0.0 3,426 - +0.5 3,458 - +0.1 3,550 - BT0005 0.17 -1.6 3,557 + BT0005 0.18 -3.3 3,638 - BT0005 0.33 -1.2 3,694 - BT0005 0.44 -3.0 3,696 + BT0005 0.44 -1.6 3,697 - BT0005 0.44 -1.2 3,746 + BT0005 0.53 -1.9 3,747 - BT0005 0.53 -0.2 3,747 - BT0005 0.53 +0.7 3,785 + BT0005 0.61 -2.9 3,872 - BT0005 0.77 +1.1 3,917 + BT0005 0.85 -0.9 3,918 - BT0005 0.85 -4.8 3,918 - BT0005 0.85 -3.2 4,104 + -1.2 4,208 - BT0006 0.27 -4.8 4,258 - BT0006 0.38 -1.2 4,278 - BT0006 0.43 +0.8 4,278 - BT0006 0.43 -0.3 4,280 - BT0006 0.43 -0.8 4,280 - BT0006 0.43 -1.9 4,291 - BT0006 0.45 +0.1 4,301 - BT0006 0.48 -2.2 4,304 + BT0006 0.48 -1.6 4,324 + BT0006 0.53 -2.5 4,335 - BT0006 0.55 -1.9 4,364 - BT0006 0.62 -1.0 4,367 + BT0006 0.62 -1.0 4,486 + -0.1 4,567 - BT0007 0.28 +0.7 4,569 - BT0007 0.28 -1.5 4,571 - BT0007 0.29 +0.8 4,714 - BT0007 0.65 -0.6 4,714 - BT0007 0.65 -0.3 4,747 + BT0007 0.74 +0.8 4,969 - -2.9
Or see this region's nucleotide sequence