Experiment: BHIS with Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt p5482_14 and p5482_15 are separated by 4 nucleotides p5482_15 and p5482_16 overlap by 4 nucleotides p5482_16 and p5482_17 are separated by 536 nucleotides p5482_17 and p5482_18 are separated by 10 nucleotides
p5482_14: p5482_14 - hypothetical protein (NCBI), at 13,508 to 14,152
_14
p5482_15: p5482_15 - TraM-like protein (NCBI), at 14,157 to 15,047
_15
p5482_16: p5482_16 - TraN-like protein (NCBI), at 15,044 to 16,141
_16
p5482_17: p5482_17 - hypothetical protein (NCBI), at 16,678 to 16,887
_17
p5482_18: p5482_18 - hypothetical protein (NCBI), at 16,898 to 17,605
_18
Position (kb) 15
16
17 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 14.108 kb on - strand at 14.110 kb on - strand at 14.110 kb on - strand at 14.110 kb on - strand at 14.128 kb on - strand at 14.151 kb on - strand at 14.157 kb on - strand at 14.161 kb on - strand at 14.295 kb on - strand, within p5482_15 at 14.295 kb on - strand, within p5482_15 at 14.331 kb on - strand, within p5482_15 at 14.382 kb on + strand, within p5482_15 at 14.403 kb on - strand, within p5482_15 at 14.406 kb on + strand, within p5482_15 at 14.407 kb on - strand, within p5482_15 at 14.429 kb on - strand, within p5482_15 at 14.446 kb on + strand, within p5482_15 at 14.447 kb on - strand, within p5482_15 at 14.447 kb on - strand, within p5482_15 at 14.560 kb on + strand, within p5482_15 at 14.561 kb on - strand, within p5482_15 at 14.657 kb on - strand, within p5482_15 at 14.705 kb on + strand, within p5482_15 at 14.706 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.760 kb on - strand, within p5482_15 at 14.761 kb on + strand, within p5482_15 at 14.796 kb on + strand, within p5482_15 at 14.797 kb on - strand, within p5482_15 at 14.841 kb on - strand, within p5482_15 at 14.841 kb on - strand, within p5482_15 at 14.841 kb on - strand, within p5482_15 at 14.841 kb on - strand, within p5482_15 at 14.843 kb on - strand, within p5482_15 at 14.843 kb on - strand, within p5482_15 at 14.843 kb on - strand, within p5482_15 at 14.844 kb on + strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.845 kb on - strand, within p5482_15 at 14.916 kb on - strand, within p5482_15 at 14.923 kb on - strand, within p5482_15 at 14.939 kb on - strand, within p5482_15 at 14.943 kb on - strand, within p5482_15 at 14.952 kb on - strand, within p5482_15 at 14.952 kb on - strand, within p5482_15 at 15.028 kb on - strand at 15.029 kb on + strand at 15.030 kb on - strand at 15.034 kb on + strand at 15.034 kb on + strand at 15.052 kb on + strand at 15.052 kb on + strand at 15.052 kb on + strand at 15.052 kb on + strand at 15.053 kb on - strand at 15.059 kb on - strand at 15.124 kb on + strand at 15.150 kb on + strand at 15.150 kb on + strand at 15.150 kb on + strand at 15.151 kb on - strand at 15.159 kb on + strand, within p5482_16 at 15.159 kb on + strand, within p5482_16 at 15.160 kb on - strand, within p5482_16 at 15.160 kb on - strand, within p5482_16 at 15.160 kb on - strand, within p5482_16 at 15.179 kb on + strand, within p5482_16 at 15.179 kb on + strand, within p5482_16 at 15.179 kb on + strand, within p5482_16 at 15.181 kb on + strand, within p5482_16 at 15.181 kb on + strand, within p5482_16 at 15.232 kb on + strand, within p5482_16 at 15.232 kb on + strand, within p5482_16 at 15.232 kb on + strand, within p5482_16 at 15.233 kb on - strand, within p5482_16 at 15.233 kb on - strand, within p5482_16 at 15.323 kb on - strand, within p5482_16 at 15.332 kb on + strand, within p5482_16 at 15.332 kb on + strand, within p5482_16 at 15.333 kb on - strand, within p5482_16 at 15.333 kb on - strand, within p5482_16 at 15.333 kb on - strand, within p5482_16 at 15.333 kb on - strand, within p5482_16 at 15.352 kb on + strand, within p5482_16 at 15.352 kb on + strand, within p5482_16 at 15.352 kb on + strand, within p5482_16 at 15.352 kb on + strand, within p5482_16 at 15.353 kb on - strand, within p5482_16 at 15.353 kb on - strand, within p5482_16 at 15.373 kb on + strand, within p5482_16 at 15.373 kb on + strand, within p5482_16 at 15.384 kb on + strand, within p5482_16 at 15.384 kb on + strand, within p5482_16 at 15.384 kb on + strand, within p5482_16 at 15.385 kb on - strand, within p5482_16 at 15.385 kb on - strand, within p5482_16 at 15.385 kb on - strand, within p5482_16 at 15.397 kb on + strand, within p5482_16 at 15.397 kb on + strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.398 kb on - strand, within p5482_16 at 15.401 kb on + strand, within p5482_16 at 15.452 kb on + strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.453 kb on - strand, within p5482_16 at 15.455 kb on - strand, within p5482_16 at 15.457 kb on - strand, within p5482_16 at 15.477 kb on + strand, within p5482_16 at 15.477 kb on + strand, within p5482_16 at 15.483 kb on + strand, within p5482_16 at 15.483 kb on + strand, within p5482_16 at 15.483 kb on + strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.484 kb on - strand, within p5482_16 at 15.509 kb on - strand, within p5482_16 at 15.509 kb on - strand, within p5482_16 at 15.597 kb on - strand, within p5482_16 at 15.597 kb on - strand, within p5482_16 at 15.649 kb on - strand, within p5482_16 at 15.738 kb on + strand, within p5482_16 at 15.739 kb on - strand, within p5482_16 at 15.740 kb on + strand, within p5482_16 at 15.740 kb on + strand, within p5482_16 at 15.740 kb on + strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.741 kb on - strand, within p5482_16 at 15.743 kb on - strand at 15.815 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.817 kb on - strand, within p5482_16 at 15.819 kb on - strand, within p5482_16 at 15.819 kb on - strand, within p5482_16 at 15.819 kb on - strand, within p5482_16 at 15.873 kb on - strand, within p5482_16 at 15.873 kb on - strand, within p5482_16 at 15.878 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.880 kb on - strand, within p5482_16 at 15.884 kb on + strand, within p5482_16 at 15.885 kb on - strand, within p5482_16 at 15.887 kb on - strand, within p5482_16 at 15.897 kb on - strand, within p5482_16 at 16.025 kb on - strand, within p5482_16 at 16.139 kb on + strand at 16.140 kb on - strand at 16.140 kb on - strand at 16.140 kb on - strand at 16.234 kb on - strand at 16.281 kb on - strand at 16.324 kb on - strand at 16.424 kb on - strand at 16.487 kb on - strand at 16.522 kb on - strand at 16.522 kb on - strand at 16.522 kb on - strand at 16.522 kb on - strand at 16.522 kb on - strand at 16.543 kb on - strand at 16.610 kb on + strand at 16.630 kb on - strand at 16.630 kb on - strand at 16.640 kb on - strand at 16.657 kb on + strand at 16.666 kb on + strand at 16.667 kb on - strand at 16.686 kb on - strand at 16.686 kb on - strand at 16.686 kb on - strand at 16.689 kb on + strand at 16.689 kb on + strand at 16.703 kb on + strand, within p5482_17 at 16.703 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.816 kb on + strand, within p5482_17 at 16.817 kb on - strand, within p5482_17 at 16.869 kb on + strand at 16.870 kb on - strand at 16.886 kb on - strand at 16.890 kb on + strand at 16.898 kb on - strand at 16.909 kb on - strand at 16.999 kb on + strand, within p5482_18 at 16.999 kb on + strand, within p5482_18 at 17.000 kb on - strand, within p5482_18 at 17.001 kb on + strand, within p5482_18 at 17.019 kb on + strand, within p5482_18 at 17.047 kb on + strand, within p5482_18 at 17.047 kb on + strand, within p5482_18 at 17.048 kb on - strand, within p5482_18 at 17.085 kb on + strand, within p5482_18 at 17.085 kb on + strand, within p5482_18 at 17.086 kb on - strand, within p5482_18 at 17.086 kb on - strand, within p5482_18
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Paraquat dichloride 0.1 mg/ml remove 14,108 - -1.7 14,110 - +0.3 14,110 - -2.2 14,110 - +1.6 14,128 - -0.8 14,151 - +0.9 14,157 - +2.1 14,161 - -0.3 14,295 - p5482_15 0.15 -1.9 14,295 - p5482_15 0.15 -1.1 14,331 - p5482_15 0.20 +2.2 14,382 + p5482_15 0.25 -0.7 14,403 - p5482_15 0.28 +0.7 14,406 + p5482_15 0.28 -0.4 14,407 - p5482_15 0.28 +0.2 14,429 - p5482_15 0.31 +0.0 14,446 + p5482_15 0.32 +1.0 14,447 - p5482_15 0.33 -1.8 14,447 - p5482_15 0.33 +0.6 14,560 + p5482_15 0.45 +0.6 14,561 - p5482_15 0.45 -0.9 14,657 - p5482_15 0.56 -1.9 14,705 + p5482_15 0.62 -2.2 14,706 - p5482_15 0.62 -0.8 14,760 - p5482_15 0.68 -0.6 14,760 - p5482_15 0.68 -0.0 14,760 - p5482_15 0.68 -0.5 14,760 - p5482_15 0.68 +2.6 14,760 - p5482_15 0.68 -0.1 14,761 + p5482_15 0.68 +1.6 14,796 + p5482_15 0.72 -0.5 14,797 - p5482_15 0.72 -1.1 14,841 - p5482_15 0.77 -2.8 14,841 - p5482_15 0.77 -0.2 14,841 - p5482_15 0.77 +1.5 14,841 - p5482_15 0.77 +0.9 14,843 - p5482_15 0.77 -3.1 14,843 - p5482_15 0.77 +2.6 14,843 - p5482_15 0.77 -1.1 14,844 + p5482_15 0.77 -0.7 14,845 - p5482_15 0.77 -0.6 14,845 - p5482_15 0.77 -0.9 14,845 - p5482_15 0.77 -2.8 14,845 - p5482_15 0.77 -2.1 14,845 - p5482_15 0.77 +2.6 14,845 - p5482_15 0.77 +1.1 14,845 - p5482_15 0.77 -2.0 14,845 - p5482_15 0.77 +0.1 14,916 - p5482_15 0.85 -0.4 14,923 - p5482_15 0.86 -1.8 14,939 - p5482_15 0.88 -0.8 14,943 - p5482_15 0.88 -0.2 14,952 - p5482_15 0.89 +0.8 14,952 - p5482_15 0.89 +0.0 15,028 - -2.4 15,029 + +1.1 15,030 - +2.1 15,034 + +2.6 15,034 + +1.2 15,052 + +1.1 15,052 + +0.1 15,052 + +2.6 15,052 + -0.5 15,053 - -1.8 15,059 - -2.6 15,124 + -0.4 15,150 + +2.6 15,150 + +0.9 15,150 + -1.5 15,151 - -0.5 15,159 + p5482_16 0.10 -2.1 15,159 + p5482_16 0.10 +3.5 15,160 - p5482_16 0.11 -3.2 15,160 - p5482_16 0.11 +1.6 15,160 - p5482_16 0.11 -3.2 15,179 + p5482_16 0.12 +1.0 15,179 + p5482_16 0.12 +0.1 15,179 + p5482_16 0.12 -2.7 15,181 + p5482_16 0.12 +2.2 15,181 + p5482_16 0.12 -2.5 15,232 + p5482_16 0.17 +1.1 15,232 + p5482_16 0.17 +2.6 15,232 + p5482_16 0.17 -0.2 15,233 - p5482_16 0.17 -2.3 15,233 - p5482_16 0.17 +0.9 15,323 - p5482_16 0.25 -3.2 15,332 + p5482_16 0.26 +1.4 15,332 + p5482_16 0.26 +1.1 15,333 - p5482_16 0.26 +1.6 15,333 - p5482_16 0.26 +0.5 15,333 - p5482_16 0.26 -0.8 15,333 - p5482_16 0.26 +0.1 15,352 + p5482_16 0.28 -0.4 15,352 + p5482_16 0.28 -0.4 15,352 + p5482_16 0.28 +2.2 15,352 + p5482_16 0.28 -2.8 15,353 - p5482_16 0.28 -1.2 15,353 - p5482_16 0.28 -0.7 15,373 + p5482_16 0.30 +0.5 15,373 + p5482_16 0.30 -1.9 15,384 + p5482_16 0.31 -0.1 15,384 + p5482_16 0.31 +1.1 15,384 + p5482_16 0.31 +1.4 15,385 - p5482_16 0.31 +1.4 15,385 - p5482_16 0.31 +2.6 15,385 - p5482_16 0.31 +0.6 15,397 + p5482_16 0.32 +1.1 15,397 + p5482_16 0.32 +1.6 15,398 - p5482_16 0.32 -1.7 15,398 - p5482_16 0.32 -0.9 15,398 - p5482_16 0.32 -0.4 15,398 - p5482_16 0.32 +1.5 15,398 - p5482_16 0.32 -0.4 15,401 + p5482_16 0.33 -1.5 15,452 + p5482_16 0.37 -2.6 15,453 - p5482_16 0.37 -1.4 15,453 - p5482_16 0.37 -1.2 15,453 - p5482_16 0.37 +0.2 15,453 - p5482_16 0.37 +2.1 15,453 - p5482_16 0.37 -0.3 15,455 - p5482_16 0.37 +0.5 15,457 - p5482_16 0.38 +2.6 15,477 + p5482_16 0.39 +0.6 15,477 + p5482_16 0.39 +1.1 15,483 + p5482_16 0.40 -1.8 15,483 + p5482_16 0.40 -0.2 15,483 + p5482_16 0.40 -2.2 15,484 - p5482_16 0.40 +0.6 15,484 - p5482_16 0.40 -0.6 15,484 - p5482_16 0.40 +0.1 15,484 - p5482_16 0.40 +1.1 15,484 - p5482_16 0.40 +1.4 15,509 - p5482_16 0.42 +1.6 15,509 - p5482_16 0.42 -0.8 15,597 - p5482_16 0.50 -0.7 15,597 - p5482_16 0.50 -1.7 15,649 - p5482_16 0.55 +0.5 15,738 + p5482_16 0.63 +1.1 15,739 - p5482_16 0.63 -1.9 15,740 + p5482_16 0.63 +3.6 15,740 + p5482_16 0.63 -0.6 15,740 + p5482_16 0.63 +0.6 15,741 - p5482_16 0.63 +0.1 15,741 - p5482_16 0.63 +2.0 15,741 - p5482_16 0.63 +1.6 15,741 - p5482_16 0.63 +2.1 15,741 - p5482_16 0.63 -2.2 15,741 - p5482_16 0.63 +1.2 15,743 - -0.7 15,815 - p5482_16 0.70 -0.8 15,817 - p5482_16 0.70 +0.7 15,817 - p5482_16 0.70 +0.3 15,817 - p5482_16 0.70 +1.3 15,817 - p5482_16 0.70 +0.9 15,817 - p5482_16 0.70 +0.2 15,817 - p5482_16 0.70 -0.1 15,819 - p5482_16 0.71 -0.5 15,819 - p5482_16 0.71 +0.7 15,819 - p5482_16 0.71 -0.2 15,873 - p5482_16 0.76 -1.4 15,873 - p5482_16 0.76 +1.9 15,878 - p5482_16 0.76 -0.5 15,880 - p5482_16 0.76 +2.2 15,880 - p5482_16 0.76 +1.4 15,880 - p5482_16 0.76 -1.8 15,880 - p5482_16 0.76 -0.8 15,880 - p5482_16 0.76 -0.8 15,880 - p5482_16 0.76 +3.6 15,884 + p5482_16 0.77 +1.2 15,885 - p5482_16 0.77 +2.2 15,887 - p5482_16 0.77 -0.9 15,897 - p5482_16 0.78 +2.6 16,025 - p5482_16 0.89 -0.9 16,139 + +1.6 16,140 - -1.2 16,140 - +1.1 16,140 - +1.6 16,234 - -1.2 16,281 - -2.5 16,324 - -0.5 16,424 - +0.8 16,487 - -2.0 16,522 - -0.6 16,522 - +0.6 16,522 - -0.8 16,522 - +1.6 16,522 - -1.5 16,543 - -3.6 16,610 + +1.7 16,630 - +2.0 16,630 - +0.4 16,640 - -0.8 16,657 + -2.0 16,666 + +0.9 16,667 - -0.9 16,686 - +0.3 16,686 - -1.6 16,686 - -0.8 16,689 + +0.1 16,689 + +1.6 16,703 + p5482_17 0.12 -0.4 16,703 + p5482_17 0.12 -1.3 16,816 + p5482_17 0.66 -0.8 16,816 + p5482_17 0.66 +0.8 16,816 + p5482_17 0.66 -0.9 16,816 + p5482_17 0.66 -2.2 16,816 + p5482_17 0.66 -1.1 16,816 + p5482_17 0.66 +0.6 16,816 + p5482_17 0.66 +0.3 16,817 - p5482_17 0.66 -1.9 16,869 + -1.7 16,870 - +2.6 16,886 - +0.6 16,890 + +0.6 16,898 - -0.8 16,909 - +0.4 16,999 + p5482_18 0.14 -0.5 16,999 + p5482_18 0.14 -1.5 17,000 - p5482_18 0.14 +1.3 17,001 + p5482_18 0.15 +0.2 17,019 + p5482_18 0.17 +0.4 17,047 + p5482_18 0.21 +0.9 17,047 + p5482_18 0.21 -2.2 17,048 - p5482_18 0.21 +2.6 17,085 + p5482_18 0.26 -0.2 17,085 + p5482_18 0.26 +0.1 17,086 - p5482_18 0.27 +2.6 17,086 - p5482_18 0.27 -0.3
Or see this region's nucleotide sequence