Experiment: Chloramphenicol 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_647 and Pf6N2E2_648 are separated by 47 nucleotides Pf6N2E2_648 and Pf6N2E2_649 overlap by 4 nucleotides Pf6N2E2_649 and Pf6N2E2_650 are separated by 16 nucleotides Pf6N2E2_650 and Pf6N2E2_651 overlap by 4 nucleotides
Pf6N2E2_647 - General secretion pathway protein G, at 736,222 to 736,671
_647
Pf6N2E2_648 - General secretion pathway protein K, at 736,719 to 737,666
_648
Pf6N2E2_649 - General secretion pathway protein L, at 737,663 to 738,835
_649
Pf6N2E2_650 - General secretion pathway protein M, at 738,852 to 739,400
_650
Pf6N2E2_651 - General secretion pathway protein D, at 739,397 to 741,760
_651
Position (kb) 737
738
739 Strain fitness (log2 ratio) -1
0
1 at 736.670 kb on - strand at 736.670 kb on - strand at 737.400 kb on + strand, within Pf6N2E2_648 at 737.468 kb on + strand, within Pf6N2E2_648 at 737.618 kb on + strand at 737.662 kb on + strand at 738.077 kb on + strand, within Pf6N2E2_649 at 738.113 kb on - strand, within Pf6N2E2_649 at 738.413 kb on + strand, within Pf6N2E2_649 at 738.834 kb on - strand at 738.834 kb on - strand at 739.008 kb on + strand, within Pf6N2E2_650 at 739.152 kb on + strand, within Pf6N2E2_650 at 739.152 kb on + strand, within Pf6N2E2_650 at 739.153 kb on - strand, within Pf6N2E2_650 at 739.153 kb on - strand, within Pf6N2E2_650 at 739.153 kb on - strand, within Pf6N2E2_650 at 739.156 kb on + strand, within Pf6N2E2_650 at 739.157 kb on - strand, within Pf6N2E2_650 at 739.597 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.004 mg/ml remove 736,670 - -0.0 736,670 - -0.6 737,400 + Pf6N2E2_648 0.72 +0.1 737,468 + Pf6N2E2_648 0.79 -0.3 737,618 + -0.4 737,662 + +0.5 738,077 + Pf6N2E2_649 0.35 -1.1 738,113 - Pf6N2E2_649 0.38 -0.5 738,413 + Pf6N2E2_649 0.64 -0.6 738,834 - +0.9 738,834 - -1.6 739,008 + Pf6N2E2_650 0.28 +0.3 739,152 + Pf6N2E2_650 0.55 +0.0 739,152 + Pf6N2E2_650 0.55 -0.4 739,153 - Pf6N2E2_650 0.55 -0.5 739,153 - Pf6N2E2_650 0.55 +0.5 739,153 - Pf6N2E2_650 0.55 +0.5 739,156 + Pf6N2E2_650 0.55 +0.1 739,157 - Pf6N2E2_650 0.56 -0.4 739,597 + +0.1
Or see this region's nucleotide sequence