Experiment: Chloramphenicol 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5480 and Pf6N2E2_5481 are separated by 25 nucleotides Pf6N2E2_5481 and Pf6N2E2_5482 are separated by 328 nucleotides Pf6N2E2_5482 and Pf6N2E2_5483 are separated by 7 nucleotides
Pf6N2E2_5480 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 6,298,058 to 6,299,113
_5480
Pf6N2E2_5481 - Transcriptional regulator, AraC family, at 6,299,139 to 6,300,068
_5481
Pf6N2E2_5482 - FIG00954888: hypothetical protein, at 6,300,397 to 6,300,750
_5482
Pf6N2E2_5483 - hypothetical protein, at 6,300,758 to 6,301,027
_5483
Position (kb)
6299
6300
6301 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.722 kb on - strand, within Pf6N2E2_5480 at 6299.026 kb on + strand at 6299.026 kb on + strand at 6299.027 kb on - strand at 6299.027 kb on - strand at 6299.080 kb on - strand at 6299.200 kb on + strand at 6299.317 kb on + strand, within Pf6N2E2_5481 at 6299.317 kb on + strand, within Pf6N2E2_5481 at 6299.317 kb on + strand, within Pf6N2E2_5481 at 6299.528 kb on - strand, within Pf6N2E2_5481 at 6299.580 kb on - strand, within Pf6N2E2_5481 at 6299.641 kb on + strand, within Pf6N2E2_5481 at 6299.641 kb on + strand, within Pf6N2E2_5481 at 6299.642 kb on - strand, within Pf6N2E2_5481 at 6299.642 kb on - strand, within Pf6N2E2_5481 at 6299.642 kb on - strand, within Pf6N2E2_5481 at 6299.642 kb on - strand, within Pf6N2E2_5481 at 6299.671 kb on - strand, within Pf6N2E2_5481 at 6299.809 kb on + strand, within Pf6N2E2_5481 at 6299.824 kb on + strand, within Pf6N2E2_5481 at 6299.825 kb on - strand, within Pf6N2E2_5481 at 6299.825 kb on - strand, within Pf6N2E2_5481 at 6299.825 kb on - strand, within Pf6N2E2_5481 at 6299.825 kb on - strand, within Pf6N2E2_5481 at 6299.912 kb on + strand, within Pf6N2E2_5481 at 6299.913 kb on - strand, within Pf6N2E2_5481 at 6299.913 kb on - strand, within Pf6N2E2_5481 at 6299.962 kb on + strand, within Pf6N2E2_5481 at 6299.963 kb on - strand, within Pf6N2E2_5481 at 6299.963 kb on - strand, within Pf6N2E2_5481 at 6299.963 kb on - strand, within Pf6N2E2_5481 at 6300.028 kb on + strand at 6300.029 kb on - strand at 6300.029 kb on - strand at 6300.109 kb on + strand at 6300.109 kb on + strand at 6300.109 kb on + strand at 6300.110 kb on - strand at 6300.110 kb on - strand at 6300.110 kb on - strand at 6300.133 kb on - strand at 6300.263 kb on + strand at 6300.263 kb on + strand at 6300.264 kb on - strand at 6300.264 kb on - strand at 6300.334 kb on + strand at 6300.334 kb on + strand at 6300.402 kb on + strand at 6300.402 kb on + strand at 6300.402 kb on + strand at 6300.403 kb on - strand at 6300.403 kb on - strand at 6300.403 kb on - strand at 6300.480 kb on + strand, within Pf6N2E2_5482 at 6300.481 kb on - strand, within Pf6N2E2_5482 at 6300.604 kb on + strand, within Pf6N2E2_5482 at 6300.605 kb on - strand, within Pf6N2E2_5482 at 6300.605 kb on - strand, within Pf6N2E2_5482 at 6300.605 kb on - strand, within Pf6N2E2_5482 at 6300.605 kb on - strand, within Pf6N2E2_5482 at 6300.629 kb on + strand, within Pf6N2E2_5482 at 6300.630 kb on - strand, within Pf6N2E2_5482 at 6300.630 kb on - strand, within Pf6N2E2_5482 at 6300.678 kb on + strand, within Pf6N2E2_5482 at 6300.678 kb on + strand, within Pf6N2E2_5482 at 6300.678 kb on + strand, within Pf6N2E2_5482 at 6300.679 kb on - strand, within Pf6N2E2_5482 at 6300.679 kb on - strand, within Pf6N2E2_5482 at 6300.731 kb on + strand at 6300.802 kb on + strand, within Pf6N2E2_5483 at 6300.802 kb on + strand, within Pf6N2E2_5483 at 6300.803 kb on - strand, within Pf6N2E2_5483 at 6300.803 kb on - strand, within Pf6N2E2_5483 at 6300.803 kb on - strand, within Pf6N2E2_5483 at 6300.837 kb on + strand, within Pf6N2E2_5483 at 6300.838 kb on - strand, within Pf6N2E2_5483
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.004 mg/ml remove 6,298,648 + Pf6N2E2_5480 0.56 +0.3 6,298,648 + Pf6N2E2_5480 0.56 -0.0 6,298,648 + Pf6N2E2_5480 0.56 +1.0 6,298,648 + Pf6N2E2_5480 0.56 +1.1 6,298,648 + Pf6N2E2_5480 0.56 +0.6 6,298,649 - Pf6N2E2_5480 0.56 +0.5 6,298,649 - Pf6N2E2_5480 0.56 +0.7 6,298,649 - Pf6N2E2_5480 0.56 +0.0 6,298,649 - Pf6N2E2_5480 0.56 -0.3 6,298,649 - Pf6N2E2_5480 0.56 -1.4 6,298,722 - Pf6N2E2_5480 0.63 -0.5 6,299,026 + +1.0 6,299,026 + +1.6 6,299,027 - -1.3 6,299,027 - -0.0 6,299,080 - -0.8 6,299,200 + +1.8 6,299,317 + Pf6N2E2_5481 0.19 +1.4 6,299,317 + Pf6N2E2_5481 0.19 +2.1 6,299,317 + Pf6N2E2_5481 0.19 +2.6 6,299,528 - Pf6N2E2_5481 0.42 +0.1 6,299,580 - Pf6N2E2_5481 0.47 +0.6 6,299,641 + Pf6N2E2_5481 0.54 +2.8 6,299,641 + Pf6N2E2_5481 0.54 +2.0 6,299,642 - Pf6N2E2_5481 0.54 +0.1 6,299,642 - Pf6N2E2_5481 0.54 +0.8 6,299,642 - Pf6N2E2_5481 0.54 -0.0 6,299,642 - Pf6N2E2_5481 0.54 +0.2 6,299,671 - Pf6N2E2_5481 0.57 -0.2 6,299,809 + Pf6N2E2_5481 0.72 +2.8 6,299,824 + Pf6N2E2_5481 0.74 +3.2 6,299,825 - Pf6N2E2_5481 0.74 -0.1 6,299,825 - Pf6N2E2_5481 0.74 -0.2 6,299,825 - Pf6N2E2_5481 0.74 +0.6 6,299,825 - Pf6N2E2_5481 0.74 +0.2 6,299,912 + Pf6N2E2_5481 0.83 +4.4 6,299,913 - Pf6N2E2_5481 0.83 -0.9 6,299,913 - Pf6N2E2_5481 0.83 -2.0 6,299,962 + Pf6N2E2_5481 0.88 +3.8 6,299,963 - Pf6N2E2_5481 0.89 +2.0 6,299,963 - Pf6N2E2_5481 0.89 +0.8 6,299,963 - Pf6N2E2_5481 0.89 +0.4 6,300,028 + +3.7 6,300,029 - -0.7 6,300,029 - +0.1 6,300,109 + +4.8 6,300,109 + +4.6 6,300,109 + +4.9 6,300,110 - +0.5 6,300,110 - +0.5 6,300,110 - +1.2 6,300,133 - +1.8 6,300,263 + +6.1 6,300,263 + +5.5 6,300,264 - +1.4 6,300,264 - +0.9 6,300,334 + +5.4 6,300,334 + +6.2 6,300,402 + +0.1 6,300,402 + -1.0 6,300,402 + -0.0 6,300,403 - -0.3 6,300,403 - -1.0 6,300,403 - +0.9 6,300,480 + Pf6N2E2_5482 0.23 +0.1 6,300,481 - Pf6N2E2_5482 0.24 +0.7 6,300,604 + Pf6N2E2_5482 0.58 +0.4 6,300,605 - Pf6N2E2_5482 0.59 +0.6 6,300,605 - Pf6N2E2_5482 0.59 +0.2 6,300,605 - Pf6N2E2_5482 0.59 +0.9 6,300,605 - Pf6N2E2_5482 0.59 +0.3 6,300,629 + Pf6N2E2_5482 0.66 -0.3 6,300,630 - Pf6N2E2_5482 0.66 +0.1 6,300,630 - Pf6N2E2_5482 0.66 -0.7 6,300,678 + Pf6N2E2_5482 0.79 -0.2 6,300,678 + Pf6N2E2_5482 0.79 -0.5 6,300,678 + Pf6N2E2_5482 0.79 +0.6 6,300,679 - Pf6N2E2_5482 0.80 -1.0 6,300,679 - Pf6N2E2_5482 0.80 +1.0 6,300,731 + -0.1 6,300,802 + Pf6N2E2_5483 0.16 +0.6 6,300,802 + Pf6N2E2_5483 0.16 -0.0 6,300,803 - Pf6N2E2_5483 0.17 -0.2 6,300,803 - Pf6N2E2_5483 0.17 +0.2 6,300,803 - Pf6N2E2_5483 0.17 -0.3 6,300,837 + Pf6N2E2_5483 0.29 +0.6 6,300,838 - Pf6N2E2_5483 0.30 -0.4
Or see this region's nucleotide sequence