Experiment: Chloramphenicol 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5091 and Pf6N2E2_5092 are separated by 89 nucleotides Pf6N2E2_5092 and Pf6N2E2_5093 are separated by 274 nucleotides Pf6N2E2_5093 and Pf6N2E2_5094 are separated by 408 nucleotides Pf6N2E2_5094 and Pf6N2E2_5095 are separated by 132 nucleotides
Pf6N2E2_5091 - putative outermembrane protein, at 5,873,119 to 5,874,972
_5091
Pf6N2E2_5092 - FIG00953225: hypothetical protein, at 5,875,062 to 5,875,550
_5092
Pf6N2E2_5093 - ATP-dependent protease La (EC 3.4.21.53) Type II, at 5,875,825 to 5,878,263
_5093
Pf6N2E2_5094 - FIG00956889: hypothetical protein, at 5,878,672 to 5,878,923
_5094
Pf6N2E2_5095 - Putative inner membrane protein (Fragment), at 5,879,056 to 5,879,544
_5095
Position (kb)
5875
5876
5877
5878
5879 Strain fitness (log2 ratio)
-2
-1
0
1 at 5874.972 kb on + strand at 5874.972 kb on + strand at 5874.973 kb on - strand at 5874.973 kb on - strand at 5874.981 kb on - strand at 5875.064 kb on - strand at 5875.064 kb on - strand at 5875.064 kb on - strand at 5875.064 kb on - strand at 5875.064 kb on - strand at 5875.079 kb on - strand at 5875.079 kb on - strand at 5875.124 kb on + strand, within Pf6N2E2_5092 at 5875.124 kb on + strand, within Pf6N2E2_5092 at 5875.125 kb on - strand, within Pf6N2E2_5092 at 5875.125 kb on - strand, within Pf6N2E2_5092 at 5875.125 kb on - strand, within Pf6N2E2_5092 at 5875.125 kb on - strand, within Pf6N2E2_5092 at 5875.125 kb on - strand, within Pf6N2E2_5092 at 5875.237 kb on + strand, within Pf6N2E2_5092 at 5875.237 kb on + strand, within Pf6N2E2_5092 at 5875.238 kb on - strand, within Pf6N2E2_5092 at 5875.238 kb on - strand, within Pf6N2E2_5092 at 5875.238 kb on - strand, within Pf6N2E2_5092 at 5875.238 kb on - strand, within Pf6N2E2_5092 at 5875.238 kb on - strand, within Pf6N2E2_5092 at 5875.328 kb on - strand, within Pf6N2E2_5092 at 5875.382 kb on + strand, within Pf6N2E2_5092 at 5875.383 kb on - strand, within Pf6N2E2_5092 at 5875.383 kb on - strand, within Pf6N2E2_5092 at 5875.512 kb on + strand at 5875.550 kb on + strand at 5875.550 kb on + strand at 5875.550 kb on + strand at 5875.550 kb on + strand at 5875.551 kb on - strand at 5875.551 kb on - strand at 5875.551 kb on - strand at 5875.551 kb on - strand at 5875.551 kb on - strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.565 kb on + strand at 5875.565 kb on + strand at 5875.565 kb on + strand at 5875.566 kb on - strand at 5875.566 kb on - strand at 5875.566 kb on - strand at 5875.566 kb on - strand at 5875.566 kb on - strand at 5875.566 kb on - strand at 5875.566 kb on - strand at 5875.597 kb on + strand at 5875.598 kb on - strand at 5875.646 kb on + strand at 5875.646 kb on + strand at 5875.738 kb on - strand at 5875.738 kb on - strand at 5875.773 kb on - strand at 5875.796 kb on - strand at 5875.796 kb on - strand at 5875.813 kb on + strand at 5875.813 kb on + strand at 5875.813 kb on + strand at 5875.813 kb on + strand at 5875.813 kb on + strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.908 kb on + strand at 5875.909 kb on - strand at 5876.002 kb on + strand at 5876.009 kb on + strand at 5876.009 kb on + strand at 5876.009 kb on + strand at 5876.009 kb on + strand at 5876.009 kb on + strand at 5876.009 kb on + strand at 5876.009 kb on + strand at 5876.010 kb on - strand at 5876.010 kb on - strand at 5876.010 kb on - strand at 5876.010 kb on - strand at 5876.059 kb on + strand at 5876.107 kb on + strand, within Pf6N2E2_5093 at 5876.108 kb on - strand, within Pf6N2E2_5093 at 5876.108 kb on - strand, within Pf6N2E2_5093 at 5876.164 kb on - strand, within Pf6N2E2_5093 at 5876.248 kb on + strand, within Pf6N2E2_5093 at 5876.249 kb on - strand, within Pf6N2E2_5093 at 5876.249 kb on - strand, within Pf6N2E2_5093 at 5876.249 kb on - strand, within Pf6N2E2_5093 at 5876.293 kb on + strand, within Pf6N2E2_5093 at 5876.294 kb on - strand, within Pf6N2E2_5093 at 5876.294 kb on - strand, within Pf6N2E2_5093 at 5876.294 kb on - strand, within Pf6N2E2_5093 at 5876.351 kb on - strand, within Pf6N2E2_5093 at 5876.351 kb on - strand, within Pf6N2E2_5093 at 5876.605 kb on + strand, within Pf6N2E2_5093 at 5876.606 kb on - strand, within Pf6N2E2_5093 at 5876.653 kb on + strand, within Pf6N2E2_5093 at 5876.653 kb on + strand, within Pf6N2E2_5093 at 5876.653 kb on + strand, within Pf6N2E2_5093 at 5876.654 kb on - strand, within Pf6N2E2_5093 at 5876.654 kb on - strand, within Pf6N2E2_5093 at 5876.654 kb on - strand, within Pf6N2E2_5093 at 5876.734 kb on + strand, within Pf6N2E2_5093 at 5876.735 kb on - strand, within Pf6N2E2_5093 at 5876.735 kb on - strand, within Pf6N2E2_5093 at 5876.735 kb on - strand, within Pf6N2E2_5093 at 5876.735 kb on - strand, within Pf6N2E2_5093 at 5876.833 kb on + strand, within Pf6N2E2_5093 at 5876.833 kb on + strand, within Pf6N2E2_5093 at 5876.833 kb on + strand, within Pf6N2E2_5093 at 5876.834 kb on - strand, within Pf6N2E2_5093 at 5876.857 kb on + strand, within Pf6N2E2_5093 at 5876.858 kb on - strand, within Pf6N2E2_5093 at 5876.858 kb on - strand, within Pf6N2E2_5093 at 5876.866 kb on + strand, within Pf6N2E2_5093 at 5876.867 kb on - strand, within Pf6N2E2_5093 at 5877.103 kb on + strand, within Pf6N2E2_5093 at 5877.103 kb on + strand, within Pf6N2E2_5093 at 5877.103 kb on + strand, within Pf6N2E2_5093 at 5877.103 kb on + strand, within Pf6N2E2_5093 at 5877.104 kb on - strand, within Pf6N2E2_5093 at 5877.104 kb on - strand, within Pf6N2E2_5093 at 5877.104 kb on - strand, within Pf6N2E2_5093 at 5877.104 kb on - strand, within Pf6N2E2_5093 at 5877.104 kb on - strand, within Pf6N2E2_5093 at 5877.106 kb on + strand, within Pf6N2E2_5093 at 5877.106 kb on + strand, within Pf6N2E2_5093 at 5877.106 kb on + strand, within Pf6N2E2_5093 at 5877.106 kb on + strand, within Pf6N2E2_5093 at 5877.107 kb on - strand, within Pf6N2E2_5093 at 5877.107 kb on - strand, within Pf6N2E2_5093 at 5877.107 kb on - strand, within Pf6N2E2_5093 at 5877.286 kb on + strand, within Pf6N2E2_5093 at 5877.286 kb on + strand, within Pf6N2E2_5093 at 5877.286 kb on + strand, within Pf6N2E2_5093 at 5877.286 kb on + strand, within Pf6N2E2_5093 at 5877.286 kb on + strand, within Pf6N2E2_5093 at 5877.287 kb on - strand, within Pf6N2E2_5093 at 5877.287 kb on - strand, within Pf6N2E2_5093 at 5877.287 kb on - strand, within Pf6N2E2_5093 at 5877.287 kb on - strand, within Pf6N2E2_5093 at 5877.411 kb on + strand, within Pf6N2E2_5093 at 5877.545 kb on + strand, within Pf6N2E2_5093 at 5877.545 kb on + strand, within Pf6N2E2_5093 at 5877.545 kb on + strand, within Pf6N2E2_5093 at 5877.546 kb on - strand, within Pf6N2E2_5093 at 5877.684 kb on + strand, within Pf6N2E2_5093 at 5877.760 kb on + strand, within Pf6N2E2_5093 at 5877.760 kb on + strand, within Pf6N2E2_5093 at 5877.761 kb on - strand, within Pf6N2E2_5093 at 5877.761 kb on - strand, within Pf6N2E2_5093 at 5877.761 kb on - strand, within Pf6N2E2_5093 at 5877.766 kb on + strand, within Pf6N2E2_5093 at 5877.804 kb on + strand, within Pf6N2E2_5093 at 5877.805 kb on - strand, within Pf6N2E2_5093 at 5878.051 kb on + strand at 5878.051 kb on + strand at 5878.051 kb on + strand at 5878.051 kb on + strand at 5878.052 kb on - strand at 5878.379 kb on - strand at 5878.393 kb on + strand at 5878.492 kb on + strand at 5878.565 kb on + strand at 5878.566 kb on - strand at 5878.566 kb on - strand at 5878.566 kb on - strand at 5878.665 kb on + strand at 5878.666 kb on - strand at 5878.671 kb on + strand at 5878.776 kb on + strand, within Pf6N2E2_5094 at 5878.807 kb on + strand, within Pf6N2E2_5094 at 5878.832 kb on + strand, within Pf6N2E2_5094 at 5878.832 kb on + strand, within Pf6N2E2_5094 at 5878.832 kb on + strand, within Pf6N2E2_5094 at 5878.832 kb on + strand, within Pf6N2E2_5094 at 5878.832 kb on + strand, within Pf6N2E2_5094 at 5878.832 kb on + strand, within Pf6N2E2_5094 at 5878.833 kb on - strand, within Pf6N2E2_5094 at 5878.833 kb on - strand, within Pf6N2E2_5094 at 5878.833 kb on - strand, within Pf6N2E2_5094 at 5878.833 kb on - strand, within Pf6N2E2_5094 at 5878.833 kb on - strand, within Pf6N2E2_5094 at 5878.833 kb on - strand, within Pf6N2E2_5094 at 5878.951 kb on + strand at 5878.952 kb on - strand at 5879.017 kb on + strand at 5879.017 kb on + strand at 5879.017 kb on + strand at 5879.017 kb on + strand at 5879.018 kb on - strand at 5879.018 kb on - strand at 5879.018 kb on - strand at 5879.018 kb on - strand at 5879.019 kb on + strand at 5879.019 kb on + strand at 5879.019 kb on + strand at 5879.019 kb on + strand at 5879.019 kb on + strand at 5879.019 kb on + strand at 5879.020 kb on - strand at 5879.020 kb on - strand at 5879.020 kb on - strand at 5879.083 kb on + strand at 5879.083 kb on + strand at 5879.083 kb on + strand at 5879.083 kb on + strand at 5879.083 kb on + strand at 5879.083 kb on + strand at 5879.083 kb on + strand at 5879.083 kb on + strand at 5879.084 kb on - strand at 5879.084 kb on - strand at 5879.084 kb on - strand at 5879.084 kb on - strand at 5879.084 kb on - strand at 5879.084 kb on - strand at 5879.113 kb on + strand, within Pf6N2E2_5095
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.004 mg/ml remove 5,874,972 + +0.9 5,874,972 + +0.6 5,874,973 - +0.5 5,874,973 - +0.1 5,874,981 - +1.5 5,875,064 - +0.7 5,875,064 - -0.2 5,875,064 - -0.5 5,875,064 - +0.2 5,875,064 - +1.7 5,875,079 - -0.7 5,875,079 - -0.0 5,875,124 + Pf6N2E2_5092 0.13 +0.5 5,875,124 + Pf6N2E2_5092 0.13 +0.0 5,875,125 - Pf6N2E2_5092 0.13 +0.1 5,875,125 - Pf6N2E2_5092 0.13 +0.2 5,875,125 - Pf6N2E2_5092 0.13 -0.1 5,875,125 - Pf6N2E2_5092 0.13 -0.2 5,875,125 - Pf6N2E2_5092 0.13 +0.1 5,875,237 + Pf6N2E2_5092 0.36 +0.5 5,875,237 + Pf6N2E2_5092 0.36 -0.7 5,875,238 - Pf6N2E2_5092 0.36 -0.1 5,875,238 - Pf6N2E2_5092 0.36 +0.6 5,875,238 - Pf6N2E2_5092 0.36 -0.1 5,875,238 - Pf6N2E2_5092 0.36 +0.6 5,875,238 - Pf6N2E2_5092 0.36 +1.0 5,875,328 - Pf6N2E2_5092 0.54 +0.4 5,875,382 + Pf6N2E2_5092 0.65 +0.0 5,875,383 - Pf6N2E2_5092 0.66 -0.0 5,875,383 - Pf6N2E2_5092 0.66 -0.2 5,875,512 + +0.7 5,875,550 + -0.1 5,875,550 + +0.8 5,875,550 + +0.3 5,875,550 + -1.6 5,875,551 - +0.6 5,875,551 - +0.6 5,875,551 - +0.5 5,875,551 - -0.5 5,875,551 - -0.0 5,875,554 + +0.1 5,875,554 + +0.5 5,875,554 + -0.1 5,875,554 + +0.4 5,875,554 + -0.1 5,875,554 + -0.2 5,875,554 + -0.2 5,875,554 + +0.1 5,875,554 + +0.3 5,875,554 + +0.1 5,875,554 + -0.4 5,875,554 + -0.2 5,875,555 - -0.2 5,875,555 - -0.0 5,875,555 - +0.3 5,875,555 - -0.3 5,875,555 - +0.7 5,875,555 - +0.2 5,875,555 - +0.5 5,875,555 - +0.3 5,875,555 - +0.7 5,875,555 - +0.5 5,875,565 + +0.2 5,875,565 + +0.3 5,875,565 + -0.0 5,875,566 - +0.4 5,875,566 - +0.2 5,875,566 - +0.0 5,875,566 - +0.1 5,875,566 - +0.6 5,875,566 - -0.2 5,875,566 - -0.5 5,875,597 + +1.0 5,875,598 - +0.1 5,875,646 + +0.3 5,875,646 + -1.3 5,875,738 - +1.4 5,875,738 - +0.5 5,875,773 - -0.2 5,875,796 - -0.7 5,875,796 - -0.5 5,875,813 + +0.3 5,875,813 + -0.1 5,875,813 + -0.1 5,875,813 + +0.6 5,875,813 + -0.1 5,875,814 - -0.2 5,875,814 - +0.7 5,875,814 - -1.6 5,875,814 - -0.5 5,875,814 - -0.8 5,875,814 - -0.5 5,875,814 - -0.1 5,875,814 - -1.3 5,875,814 - -0.4 5,875,814 - -0.7 5,875,814 - -1.2 5,875,814 - -0.1 5,875,908 + -0.6 5,875,909 - -0.7 5,876,002 + -0.6 5,876,009 + -0.4 5,876,009 + -0.6 5,876,009 + -0.5 5,876,009 + -0.7 5,876,009 + -1.1 5,876,009 + -1.1 5,876,009 + -0.0 5,876,010 - -1.1 5,876,010 - -0.6 5,876,010 - -0.3 5,876,010 - -0.7 5,876,059 + -1.4 5,876,107 + Pf6N2E2_5093 0.12 -0.5 5,876,108 - Pf6N2E2_5093 0.12 -1.0 5,876,108 - Pf6N2E2_5093 0.12 -0.8 5,876,164 - Pf6N2E2_5093 0.14 -0.3 5,876,248 + Pf6N2E2_5093 0.17 -1.4 5,876,249 - Pf6N2E2_5093 0.17 -1.2 5,876,249 - Pf6N2E2_5093 0.17 -0.8 5,876,249 - Pf6N2E2_5093 0.17 -0.3 5,876,293 + Pf6N2E2_5093 0.19 -0.7 5,876,294 - Pf6N2E2_5093 0.19 -0.4 5,876,294 - Pf6N2E2_5093 0.19 -0.9 5,876,294 - Pf6N2E2_5093 0.19 -0.9 5,876,351 - Pf6N2E2_5093 0.22 -0.5 5,876,351 - Pf6N2E2_5093 0.22 -0.3 5,876,605 + Pf6N2E2_5093 0.32 -1.3 5,876,606 - Pf6N2E2_5093 0.32 -0.5 5,876,653 + Pf6N2E2_5093 0.34 -0.9 5,876,653 + Pf6N2E2_5093 0.34 -1.1 5,876,653 + Pf6N2E2_5093 0.34 -0.6 5,876,654 - Pf6N2E2_5093 0.34 -0.9 5,876,654 - Pf6N2E2_5093 0.34 -1.6 5,876,654 - Pf6N2E2_5093 0.34 +0.4 5,876,734 + Pf6N2E2_5093 0.37 -0.4 5,876,735 - Pf6N2E2_5093 0.37 -0.4 5,876,735 - Pf6N2E2_5093 0.37 -0.6 5,876,735 - Pf6N2E2_5093 0.37 -0.2 5,876,735 - Pf6N2E2_5093 0.37 -0.4 5,876,833 + Pf6N2E2_5093 0.41 -0.3 5,876,833 + Pf6N2E2_5093 0.41 -0.9 5,876,833 + Pf6N2E2_5093 0.41 -0.5 5,876,834 - Pf6N2E2_5093 0.41 -1.2 5,876,857 + Pf6N2E2_5093 0.42 -1.2 5,876,858 - Pf6N2E2_5093 0.42 -0.8 5,876,858 - Pf6N2E2_5093 0.42 -1.3 5,876,866 + Pf6N2E2_5093 0.43 -1.1 5,876,867 - Pf6N2E2_5093 0.43 -1.4 5,877,103 + Pf6N2E2_5093 0.52 -1.1 5,877,103 + Pf6N2E2_5093 0.52 -1.8 5,877,103 + Pf6N2E2_5093 0.52 -1.8 5,877,103 + Pf6N2E2_5093 0.52 -0.9 5,877,104 - Pf6N2E2_5093 0.52 -1.4 5,877,104 - Pf6N2E2_5093 0.52 -0.8 5,877,104 - Pf6N2E2_5093 0.52 -0.2 5,877,104 - Pf6N2E2_5093 0.52 -0.9 5,877,104 - Pf6N2E2_5093 0.52 -1.2 5,877,106 + Pf6N2E2_5093 0.53 -0.3 5,877,106 + Pf6N2E2_5093 0.53 +0.1 5,877,106 + Pf6N2E2_5093 0.53 -0.6 5,877,106 + Pf6N2E2_5093 0.53 -0.7 5,877,107 - Pf6N2E2_5093 0.53 -0.6 5,877,107 - Pf6N2E2_5093 0.53 -1.2 5,877,107 - Pf6N2E2_5093 0.53 -0.9 5,877,286 + Pf6N2E2_5093 0.60 -1.1 5,877,286 + Pf6N2E2_5093 0.60 -1.1 5,877,286 + Pf6N2E2_5093 0.60 -1.8 5,877,286 + Pf6N2E2_5093 0.60 -0.6 5,877,286 + Pf6N2E2_5093 0.60 -1.0 5,877,287 - Pf6N2E2_5093 0.60 -0.5 5,877,287 - Pf6N2E2_5093 0.60 -1.4 5,877,287 - Pf6N2E2_5093 0.60 -0.4 5,877,287 - Pf6N2E2_5093 0.60 -2.7 5,877,411 + Pf6N2E2_5093 0.65 -0.2 5,877,545 + Pf6N2E2_5093 0.71 -0.6 5,877,545 + Pf6N2E2_5093 0.71 -1.8 5,877,545 + Pf6N2E2_5093 0.71 -1.1 5,877,546 - Pf6N2E2_5093 0.71 -1.1 5,877,684 + Pf6N2E2_5093 0.76 -0.4 5,877,760 + Pf6N2E2_5093 0.79 -1.3 5,877,760 + Pf6N2E2_5093 0.79 +0.0 5,877,761 - Pf6N2E2_5093 0.79 -0.1 5,877,761 - Pf6N2E2_5093 0.79 -0.4 5,877,761 - Pf6N2E2_5093 0.79 -1.0 5,877,766 + Pf6N2E2_5093 0.80 -0.3 5,877,804 + Pf6N2E2_5093 0.81 -2.1 5,877,805 - Pf6N2E2_5093 0.81 -1.2 5,878,051 + -0.8 5,878,051 + -0.7 5,878,051 + -0.5 5,878,051 + +0.0 5,878,052 - -1.1 5,878,379 - -0.1 5,878,393 + +0.8 5,878,492 + -0.5 5,878,565 + +0.1 5,878,566 - +0.5 5,878,566 - -0.5 5,878,566 - +0.5 5,878,665 + +1.6 5,878,666 - +0.9 5,878,671 + +0.2 5,878,776 + Pf6N2E2_5094 0.41 +0.8 5,878,807 + Pf6N2E2_5094 0.54 -0.2 5,878,832 + Pf6N2E2_5094 0.63 +0.7 5,878,832 + Pf6N2E2_5094 0.63 -0.5 5,878,832 + Pf6N2E2_5094 0.63 +1.0 5,878,832 + Pf6N2E2_5094 0.63 +0.2 5,878,832 + Pf6N2E2_5094 0.63 +0.1 5,878,832 + Pf6N2E2_5094 0.63 +0.6 5,878,833 - Pf6N2E2_5094 0.64 +0.5 5,878,833 - Pf6N2E2_5094 0.64 +0.1 5,878,833 - Pf6N2E2_5094 0.64 +0.2 5,878,833 - Pf6N2E2_5094 0.64 -0.1 5,878,833 - Pf6N2E2_5094 0.64 -0.9 5,878,833 - Pf6N2E2_5094 0.64 -0.7 5,878,951 + +0.1 5,878,952 - +0.3 5,879,017 + +0.6 5,879,017 + -0.5 5,879,017 + -0.3 5,879,017 + -0.8 5,879,018 - -0.1 5,879,018 - -0.3 5,879,018 - +0.3 5,879,018 - +0.0 5,879,019 + +0.5 5,879,019 + -0.2 5,879,019 + +0.2 5,879,019 + +0.2 5,879,019 + -0.6 5,879,019 + +0.2 5,879,020 - +0.5 5,879,020 - -1.1 5,879,020 - -0.6 5,879,083 + -0.0 5,879,083 + -1.2 5,879,083 + +0.1 5,879,083 + +0.3 5,879,083 + -1.3 5,879,083 + +0.3 5,879,083 + +0.2 5,879,083 + -0.7 5,879,084 - -0.7 5,879,084 - +0.3 5,879,084 - +0.8 5,879,084 - -0.7 5,879,084 - +0.1 5,879,084 - +0.7 5,879,113 + Pf6N2E2_5095 0.12 -0.3
Or see this region's nucleotide sequence