Strain Fitness in Methanococcus maripaludis S2 around MMP_RS07005

Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS06995 and MMP_RS07000 are separated by 15 nucleotidesMMP_RS07000 and MMP_RS07005 are separated by 44 nucleotidesMMP_RS07005 and MMP_RS07010 are separated by 13 nucleotides MMP_RS06995: MMP_RS06995 - TIGR00269 family protein, at 1,335,578 to 1,336,513 _RS06995 MMP_RS07000: MMP_RS07000 - MoaD/ThiS family protein, at 1,336,529 to 1,336,783 _RS07000 MMP_RS07005: MMP_RS07005 - 4Fe-4S binding protein, at 1,336,828 to 1,337,226 _RS07005 MMP_RS07010: MMP_RS07010 - homocysteine biosynthesis protein, at 1,337,240 to 1,338,781 _RS07010 Position (kb) 1336 1337 1338Strain fitness (log2 ratio) -3 -2 -1 0 1at 1336.447 kb on - strandat 1336.448 kb on + strandat 1336.480 kb on + strandat 1336.481 kb on - strandat 1336.517 kb on + strandat 1336.551 kb on - strandat 1336.553 kb on - strandat 1336.553 kb on - strandat 1336.580 kb on - strandat 1336.581 kb on + strandat 1336.622 kb on - strandat 1336.685 kb on - strandat 1336.827 kb on + strandat 1337.128 kb on - strand, within MMP_RS07005at 1337.405 kb on - strandat 1337.406 kb on + strandat 1337.407 kb on - strandat 1337.407 kb on - strandat 1337.407 kb on - strandat 1337.408 kb on + strandat 1337.409 kb on - strandat 1337.409 kb on - strandat 1337.410 kb on + strandat 1337.410 kb on + strandat 1337.414 kb on + strandat 1337.683 kb on - strandat 1337.835 kb on - strandat 1337.836 kb on + strandat 1338.159 kb on - strandat 1338.159 kb on - strandat 1338.201 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
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1,336,447 - -2.7
1,336,448 + -2.3
1,336,480 + -3.0
1,336,481 - -2.3
1,336,517 + -2.6
1,336,551 - -1.6
1,336,553 - -1.7
1,336,553 - -1.1
1,336,580 - -1.0
1,336,581 + -1.7
1,336,622 - -1.5
1,336,685 - -2.2
1,336,827 + -1.6
1,337,128 - MMP_RS07005 0.75 -2.8
1,337,405 - -3.0
1,337,406 + -2.2
1,337,407 - -2.6
1,337,407 - -2.7
1,337,407 - -1.9
1,337,408 + -2.6
1,337,409 - -1.3
1,337,409 - -2.2
1,337,410 + -1.8
1,337,410 + -1.1
1,337,414 + -2.9
1,337,683 - -2.5
1,337,835 - -1.3
1,337,836 + -3.1
1,338,159 - -2.1
1,338,159 - -3.0
1,338,201 + -2.0

Or see this region's nucleotide sequence