Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06280 and MMP_RS06285 are separated by 97 nucleotides MMP_RS06285 and MMP_RS06290 are separated by 148 nucleotides
MMP_RS06280: MMP_RS06280 - alpha-glucan family phosphorylase, at 1,208,499 to 1,210,061
_RS06280
MMP_RS06285: MMP_RS06285 - precorrin-6Y C5 15-methyltransferase (decarboxylating) subunit CbiT, at 1,210,159 to 1,210,704
_RS06285
MMP_RS06290: MMP_RS06290 - DNA repair and recombination protein RadA, at 1,210,853 to 1,211,821
_RS06290
Position (kb)
1210
1211 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1209.230 kb on + strand, within MMP_RS06280 at 1209.613 kb on - strand, within MMP_RS06280 at 1209.627 kb on - strand, within MMP_RS06280 at 1209.957 kb on + strand at 1210.003 kb on - strand at 1210.004 kb on + strand at 1210.004 kb on + strand at 1210.045 kb on - strand at 1210.105 kb on - strand at 1210.109 kb on + strand at 1210.116 kb on - strand at 1210.123 kb on - strand at 1210.123 kb on - strand at 1210.123 kb on - strand at 1210.123 kb on - strand at 1210.124 kb on + strand at 1210.124 kb on + strand at 1210.131 kb on + strand at 1210.134 kb on - strand at 1210.135 kb on + strand at 1210.135 kb on + strand at 1210.137 kb on + strand at 1210.140 kb on - strand at 1210.140 kb on - strand at 1210.141 kb on + strand at 1210.170 kb on - strand at 1210.171 kb on + strand at 1210.236 kb on + strand, within MMP_RS06285 at 1210.252 kb on - strand, within MMP_RS06285 at 1210.262 kb on + strand, within MMP_RS06285 at 1210.637 kb on + strand, within MMP_RS06285 at 1210.741 kb on - strand at 1210.764 kb on - strand at 1210.765 kb on + strand at 1210.765 kb on + strand at 1210.765 kb on + strand at 1210.769 kb on - strand at 1210.775 kb on + strand at 1210.801 kb on + strand at 1210.814 kb on - strand at 1210.814 kb on - strand at 1210.815 kb on + strand at 1210.815 kb on + strand at 1210.815 kb on + strand at 1210.835 kb on - strand at 1210.965 kb on - strand, within MMP_RS06290 at 1211.028 kb on - strand, within MMP_RS06290 at 1211.029 kb on + strand, within MMP_RS06290 at 1211.112 kb on - strand, within MMP_RS06290 at 1211.569 kb on - strand, within MMP_RS06290 at 1211.593 kb on + strand, within MMP_RS06290
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 1,209,230 + MMP_RS06280 0.47 -0.0 1,209,613 - MMP_RS06280 0.71 +0.1 1,209,627 - MMP_RS06280 0.72 +0.3 1,209,957 + -0.1 1,210,003 - -0.4 1,210,004 + +0.3 1,210,004 + +0.9 1,210,045 - +0.1 1,210,105 - -0.3 1,210,109 + +0.2 1,210,116 - +0.0 1,210,123 - +1.5 1,210,123 - +0.1 1,210,123 - +1.0 1,210,123 - -0.3 1,210,124 + -0.2 1,210,124 + -0.3 1,210,131 + +0.3 1,210,134 - +0.1 1,210,135 + -0.1 1,210,135 + -0.3 1,210,137 + +1.2 1,210,140 - -0.3 1,210,140 - +0.2 1,210,141 + -0.4 1,210,170 - +0.3 1,210,171 + -0.2 1,210,236 + MMP_RS06285 0.14 -2.8 1,210,252 - MMP_RS06285 0.17 -2.4 1,210,262 + MMP_RS06285 0.19 -0.9 1,210,637 + MMP_RS06285 0.88 -1.7 1,210,741 - +0.5 1,210,764 - +0.5 1,210,765 + +0.2 1,210,765 + -0.7 1,210,765 + -0.2 1,210,769 - +0.5 1,210,775 + +0.0 1,210,801 + -0.5 1,210,814 - -1.1 1,210,814 - -0.4 1,210,815 + +0.1 1,210,815 + -0.9 1,210,815 + +0.0 1,210,835 - +0.2 1,210,965 - MMP_RS06290 0.12 -0.4 1,211,028 - MMP_RS06290 0.18 -0.8 1,211,029 + MMP_RS06290 0.18 +0.1 1,211,112 - MMP_RS06290 0.27 -0.0 1,211,569 - MMP_RS06290 0.74 -0.2 1,211,593 + MMP_RS06290 0.76 -0.3
Or see this region's nucleotide sequence