Strain Fitness in Methanococcus maripaludis S2 around MMP_RS06215

Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS06210 and MMP_RS06215 are separated by 209 nucleotidesMMP_RS06215 and MMP_RS06220 are separated by 161 nucleotides MMP_RS06210: MMP_RS06210 - type I glutamate--ammonia ligase, at 1,192,748 to 1,194,088 _RS06210 MMP_RS06215: MMP_RS06215 - 30S ribosomal protein S6e, at 1,194,298 to 1,194,672 _RS06215 MMP_RS06220: MMP_RS06220 - translation initiation factor IF-2 subunit gamma, at 1,194,834 to 1,196,066 _RS06220 Position (kb) 1194 1195Strain fitness (log2 ratio) -3 -2 -1 0 1at 1193.305 kb on + strandat 1193.557 kb on + strandat 1194.105 kb on + strandat 1194.116 kb on + strandat 1194.187 kb on + strandat 1194.192 kb on - strandat 1194.271 kb on - strandat 1194.271 kb on - strandat 1194.272 kb on + strandat 1194.510 kb on + strand, within MMP_RS06215at 1194.510 kb on + strand, within MMP_RS06215at 1194.597 kb on - strand, within MMP_RS06215at 1194.719 kb on - strandat 1194.720 kb on + strandat 1194.721 kb on - strandat 1194.722 kb on + strandat 1194.724 kb on + strandat 1194.727 kb on - strandat 1194.727 kb on - strandat 1194.727 kb on - strandat 1194.728 kb on + strandat 1195.083 kb on + strandat 1195.513 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
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1,193,305 + -2.0
1,193,557 + -2.7
1,194,105 + -0.2
1,194,116 + +0.7
1,194,187 + -2.8
1,194,192 - -0.7
1,194,271 - -0.1
1,194,271 - -1.4
1,194,272 + -0.9
1,194,510 + MMP_RS06215 0.57 -1.1
1,194,510 + MMP_RS06215 0.57 -1.4
1,194,597 - MMP_RS06215 0.80 -1.0
1,194,719 - -0.4
1,194,720 + -0.1
1,194,721 - -0.3
1,194,722 + +0.2
1,194,724 + -0.1
1,194,727 - -3.2
1,194,727 - -3.1
1,194,727 - -2.2
1,194,728 + -1.5
1,195,083 + -1.9
1,195,513 - -2.1

Or see this region's nucleotide sequence