Experiment: PYE with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00386 and CCNA_00387 are separated by 129 nucleotides CCNA_00387 and CCNA_00388 are separated by 127 nucleotides CCNA_00388 and CCNA_00389 are separated by 97 nucleotides CCNA_00389 and CCNA_00390 are separated by 103 nucleotides
CCNA_00386: CCNA_00386 - DNA-3-methyladenine glycosylase, at 402,858 to 403,445
_00386
CCNA_00387: CCNA_00387 - hypothetical protein, at 403,575 to 403,997
_00387
CCNA_00388: CCNA_00388 - esterase lipase Family protein, at 404,125 to 405,006
_00388
CCNA_00389: CCNA_00389 - aminomethyltransferase family protein, at 405,104 to 405,895
_00389
CCNA_00390: CCNA_00390 - ADP-heptose--LPS heptosyltransferase, at 405,999 to 407,213
_00390
Position (kb) 404
405
406 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 403.140 kb on + strand, within CCNA_00386 at 403.177 kb on + strand, within CCNA_00386 at 403.181 kb on - strand, within CCNA_00386 at 403.185 kb on - strand, within CCNA_00386 at 403.185 kb on - strand, within CCNA_00386 at 403.275 kb on - strand, within CCNA_00386 at 403.345 kb on + strand, within CCNA_00386 at 403.348 kb on - strand, within CCNA_00386 at 403.372 kb on - strand, within CCNA_00386 at 403.395 kb on - strand at 403.408 kb on - strand at 403.457 kb on + strand at 403.465 kb on - strand at 403.549 kb on - strand at 403.550 kb on + strand at 403.550 kb on + strand at 403.558 kb on - strand at 403.583 kb on + strand at 403.583 kb on + strand at 403.583 kb on + strand at 403.591 kb on - strand at 403.591 kb on - strand at 403.639 kb on + strand, within CCNA_00387 at 403.639 kb on + strand, within CCNA_00387 at 403.639 kb on + strand, within CCNA_00387 at 403.639 kb on + strand, within CCNA_00387 at 403.639 kb on + strand, within CCNA_00387 at 403.644 kb on + strand, within CCNA_00387 at 403.654 kb on + strand, within CCNA_00387 at 403.657 kb on - strand, within CCNA_00387 at 403.679 kb on + strand, within CCNA_00387 at 403.809 kb on + strand, within CCNA_00387 at 403.827 kb on + strand, within CCNA_00387 at 403.842 kb on + strand, within CCNA_00387 at 403.850 kb on - strand, within CCNA_00387 at 403.866 kb on + strand, within CCNA_00387 at 403.930 kb on + strand, within CCNA_00387 at 403.965 kb on - strand at 403.976 kb on - strand at 403.997 kb on - strand at 404.029 kb on - strand at 404.030 kb on - strand at 404.125 kb on - strand at 404.148 kb on - strand at 404.193 kb on - strand at 404.199 kb on + strand at 404.214 kb on + strand, within CCNA_00388 at 404.222 kb on - strand, within CCNA_00388 at 404.230 kb on - strand, within CCNA_00388 at 404.230 kb on - strand, within CCNA_00388 at 404.230 kb on - strand, within CCNA_00388 at 404.247 kb on + strand, within CCNA_00388 at 404.290 kb on + strand, within CCNA_00388 at 404.290 kb on + strand, within CCNA_00388 at 404.305 kb on - strand, within CCNA_00388 at 404.318 kb on + strand, within CCNA_00388 at 404.346 kb on + strand, within CCNA_00388 at 404.349 kb on - strand, within CCNA_00388 at 404.374 kb on - strand, within CCNA_00388 at 404.374 kb on - strand, within CCNA_00388 at 404.374 kb on - strand, within CCNA_00388 at 404.407 kb on - strand, within CCNA_00388 at 404.415 kb on - strand, within CCNA_00388 at 404.415 kb on - strand, within CCNA_00388 at 404.416 kb on - strand, within CCNA_00388 at 404.435 kb on - strand, within CCNA_00388 at 404.436 kb on - strand, within CCNA_00388 at 404.438 kb on + strand, within CCNA_00388 at 404.446 kb on - strand, within CCNA_00388 at 404.455 kb on - strand, within CCNA_00388 at 404.482 kb on - strand, within CCNA_00388 at 404.564 kb on + strand, within CCNA_00388 at 404.608 kb on - strand, within CCNA_00388 at 404.619 kb on + strand, within CCNA_00388 at 404.619 kb on + strand, within CCNA_00388 at 404.619 kb on + strand, within CCNA_00388 at 404.619 kb on + strand, within CCNA_00388 at 404.619 kb on + strand, within CCNA_00388 at 404.627 kb on - strand, within CCNA_00388 at 404.627 kb on - strand, within CCNA_00388 at 404.627 kb on - strand, within CCNA_00388 at 404.640 kb on + strand, within CCNA_00388 at 404.640 kb on + strand, within CCNA_00388 at 404.648 kb on - strand, within CCNA_00388 at 404.648 kb on - strand, within CCNA_00388 at 404.648 kb on - strand, within CCNA_00388 at 404.671 kb on + strand, within CCNA_00388 at 404.674 kb on + strand, within CCNA_00388 at 404.679 kb on - strand, within CCNA_00388 at 404.679 kb on - strand, within CCNA_00388 at 404.696 kb on - strand, within CCNA_00388 at 404.697 kb on - strand, within CCNA_00388 at 404.697 kb on - strand, within CCNA_00388 at 404.704 kb on - strand, within CCNA_00388 at 404.704 kb on - strand, within CCNA_00388 at 404.704 kb on - strand, within CCNA_00388 at 404.714 kb on - strand, within CCNA_00388 at 404.722 kb on + strand, within CCNA_00388 at 404.728 kb on + strand, within CCNA_00388 at 404.733 kb on + strand, within CCNA_00388 at 404.752 kb on - strand, within CCNA_00388 at 404.785 kb on - strand, within CCNA_00388 at 404.812 kb on + strand, within CCNA_00388 at 404.858 kb on + strand, within CCNA_00388 at 404.907 kb on + strand, within CCNA_00388 at 404.912 kb on + strand, within CCNA_00388 at 404.936 kb on + strand at 404.943 kb on - strand at 404.953 kb on - strand at 404.968 kb on - strand at 405.103 kb on - strand at 405.119 kb on - strand at 405.313 kb on - strand, within CCNA_00389 at 405.428 kb on + strand, within CCNA_00389 at 405.452 kb on + strand, within CCNA_00389 at 405.516 kb on - strand, within CCNA_00389 at 405.724 kb on + strand, within CCNA_00389 at 405.941 kb on - strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.983 kb on + strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.991 kb on - strand at 405.999 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Doxycycline hyclate 0.0001 mg/ml remove 403,140 + CCNA_00386 0.48 -0.3 403,177 + CCNA_00386 0.54 -1.4 403,181 - CCNA_00386 0.55 +0.0 403,185 - CCNA_00386 0.56 -0.0 403,185 - CCNA_00386 0.56 -2.1 403,275 - CCNA_00386 0.71 -0.4 403,345 + CCNA_00386 0.83 -1.2 403,348 - CCNA_00386 0.83 -0.5 403,372 - CCNA_00386 0.87 -1.1 403,395 - -1.2 403,408 - -1.3 403,457 + -1.2 403,465 - +1.0 403,549 - -0.0 403,550 + -0.5 403,550 + -0.0 403,558 - +0.1 403,583 + -0.0 403,583 + -3.7 403,583 + -0.1 403,591 - +1.8 403,591 - -0.1 403,639 + CCNA_00387 0.15 -0.0 403,639 + CCNA_00387 0.15 -0.4 403,639 + CCNA_00387 0.15 -2.7 403,639 + CCNA_00387 0.15 +0.8 403,639 + CCNA_00387 0.15 -0.5 403,644 + CCNA_00387 0.16 -1.6 403,654 + CCNA_00387 0.19 +1.0 403,657 - CCNA_00387 0.19 -0.3 403,679 + CCNA_00387 0.25 -0.4 403,809 + CCNA_00387 0.55 +1.9 403,827 + CCNA_00387 0.60 +0.5 403,842 + CCNA_00387 0.63 +0.3 403,850 - CCNA_00387 0.65 -1.6 403,866 + CCNA_00387 0.69 -0.2 403,930 + CCNA_00387 0.84 +0.0 403,965 - +0.4 403,976 - +0.4 403,997 - +0.2 404,029 - +1.7 404,030 - +0.0 404,125 - +1.6 404,148 - -0.9 404,193 - -0.1 404,199 + +1.0 404,214 + CCNA_00388 0.10 +1.2 404,222 - CCNA_00388 0.11 +0.9 404,230 - CCNA_00388 0.12 -0.8 404,230 - CCNA_00388 0.12 +0.9 404,230 - CCNA_00388 0.12 -0.2 404,247 + CCNA_00388 0.14 -1.2 404,290 + CCNA_00388 0.19 +1.0 404,290 + CCNA_00388 0.19 -0.0 404,305 - CCNA_00388 0.20 -0.6 404,318 + CCNA_00388 0.22 -1.5 404,346 + CCNA_00388 0.25 -1.1 404,349 - CCNA_00388 0.25 -0.1 404,374 - CCNA_00388 0.28 +0.2 404,374 - CCNA_00388 0.28 -0.8 404,374 - CCNA_00388 0.28 +0.4 404,407 - CCNA_00388 0.32 +0.2 404,415 - CCNA_00388 0.33 +0.4 404,415 - CCNA_00388 0.33 +0.2 404,416 - CCNA_00388 0.33 +0.3 404,435 - CCNA_00388 0.35 +0.9 404,436 - CCNA_00388 0.35 -1.3 404,438 + CCNA_00388 0.35 -0.2 404,446 - CCNA_00388 0.36 +0.1 404,455 - CCNA_00388 0.37 +1.2 404,482 - CCNA_00388 0.40 +0.5 404,564 + CCNA_00388 0.50 -1.1 404,608 - CCNA_00388 0.55 +0.7 404,619 + CCNA_00388 0.56 -0.2 404,619 + CCNA_00388 0.56 -0.6 404,619 + CCNA_00388 0.56 +0.2 404,619 + CCNA_00388 0.56 -0.9 404,619 + CCNA_00388 0.56 -0.9 404,627 - CCNA_00388 0.57 +0.2 404,627 - CCNA_00388 0.57 +1.5 404,627 - CCNA_00388 0.57 +0.5 404,640 + CCNA_00388 0.58 -1.0 404,640 + CCNA_00388 0.58 -0.1 404,648 - CCNA_00388 0.59 +0.8 404,648 - CCNA_00388 0.59 +2.6 404,648 - CCNA_00388 0.59 -0.4 404,671 + CCNA_00388 0.62 -2.3 404,674 + CCNA_00388 0.62 -0.7 404,679 - CCNA_00388 0.63 +0.3 404,679 - CCNA_00388 0.63 +0.3 404,696 - CCNA_00388 0.65 -0.8 404,697 - CCNA_00388 0.65 +2.4 404,697 - CCNA_00388 0.65 -0.2 404,704 - CCNA_00388 0.66 +0.8 404,704 - CCNA_00388 0.66 -1.0 404,704 - CCNA_00388 0.66 +1.1 404,714 - CCNA_00388 0.67 +1.6 404,722 + CCNA_00388 0.68 +0.2 404,728 + CCNA_00388 0.68 +1.6 404,733 + CCNA_00388 0.69 -1.5 404,752 - CCNA_00388 0.71 -0.4 404,785 - CCNA_00388 0.75 +0.3 404,812 + CCNA_00388 0.78 -1.7 404,858 + CCNA_00388 0.83 -0.4 404,907 + CCNA_00388 0.89 -1.8 404,912 + CCNA_00388 0.89 +0.8 404,936 + -0.1 404,943 - +1.1 404,953 - +0.4 404,968 - +0.1 405,103 - -1.9 405,119 - +1.3 405,313 - CCNA_00389 0.26 +3.6 405,428 + CCNA_00389 0.41 +2.4 405,452 + CCNA_00389 0.44 +1.8 405,516 - CCNA_00389 0.52 +4.9 405,724 + CCNA_00389 0.78 +0.8 405,941 - -0.5 405,983 + -1.8 405,983 + +0.4 405,983 + -0.6 405,983 + -0.4 405,983 + -1.2 405,983 + -0.5 405,983 + -0.0 405,983 + -1.0 405,983 + -1.0 405,983 + -1.7 405,983 + -0.4 405,983 + -1.3 405,983 + +0.5 405,983 + +0.6 405,983 + -1.3 405,983 + +0.4 405,983 + +1.5 405,983 + -0.7 405,983 + -1.0 405,983 + -0.4 405,983 + -0.0 405,983 + +0.4 405,983 + -0.8 405,983 + -1.6 405,983 + +1.0 405,983 + -1.7 405,983 + -0.7 405,983 + +1.1 405,983 + -2.7 405,983 + -1.8 405,983 + -0.7 405,983 + -1.0 405,983 + -0.2 405,991 - -0.2 405,991 - +0.1 405,991 - -0.2 405,991 - -1.2 405,991 - +0.1 405,991 - -0.5 405,991 - +1.3 405,991 - -2.7 405,991 - -0.4 405,991 - -0.2 405,991 - -0.1 405,991 - -0.4 405,991 - -1.3 405,991 - -1.8 405,991 - +0.1 405,991 - -1.0 405,991 - -1.7 405,991 - +0.8 405,991 - -0.8 405,991 - -1.9 405,991 - +0.8 405,991 - -0.3 405,991 - -2.2 405,991 - +1.3 405,991 - +1.3 405,991 - -0.8 405,991 - +0.2 405,991 - -0.6 405,999 + -0.1
Or see this region's nucleotide sequence